Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pssA and yfiM are separated by 45 nucleotides yfiM and kgtP overlap by 4 nucleotides kgtP and rrfG are separated by 322 nucleotides rrfG and rrlG are separated by 92 nucleotides
b2585: pssA - phosphatidylserine synthase; phospholipid synthesis (VIMSS), at 2,720,749 to 2,722,104
pssA
b2586: yfiM - predicted protein (RefSeq), at 2,722,150 to 2,722,473
yfiM
b2587: kgtP - alpha-ketoglutarate transporter (NCBI), at 2,722,470 to 2,723,768
kgtP
b2588: rrfG - 5S ribosomal RNA (NCBI), at 2,724,091 to 2,724,210
rrfG
b2589: rrlG - 23S ribosomal RNA (NCBI), at 2,724,303 to 2,727,206
rrlG
Position (kb)
2722
2723
2724 Strain fitness (log2 ratio)
-2
-1
0
1 at 2722.110 kb on + strand at 2722.113 kb on - strand at 2722.115 kb on + strand at 2722.171 kb on + strand at 2722.227 kb on + strand, within yfiM at 2722.332 kb on + strand, within yfiM at 2722.387 kb on - strand, within yfiM at 2722.415 kb on + strand, within yfiM at 2722.435 kb on + strand, within yfiM at 2722.539 kb on - strand at 2722.539 kb on - strand at 2722.557 kb on + strand at 2722.557 kb on + strand at 2722.570 kb on + strand at 2722.604 kb on - strand, within kgtP at 2722.604 kb on - strand, within kgtP at 2722.633 kb on + strand, within kgtP at 2722.641 kb on - strand, within kgtP at 2722.783 kb on + strand, within kgtP at 2722.801 kb on + strand, within kgtP at 2722.943 kb on + strand, within kgtP at 2722.943 kb on + strand, within kgtP at 2723.060 kb on - strand, within kgtP at 2723.060 kb on - strand, within kgtP at 2723.145 kb on + strand, within kgtP at 2723.210 kb on - strand, within kgtP at 2723.235 kb on + strand, within kgtP at 2723.348 kb on - strand, within kgtP at 2723.408 kb on - strand, within kgtP at 2723.418 kb on + strand, within kgtP at 2723.418 kb on + strand, within kgtP at 2723.436 kb on + strand, within kgtP at 2723.457 kb on - strand, within kgtP at 2723.698 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 2,722,110 + +0.1 2,722,113 - +0.0 2,722,115 + -0.1 2,722,171 + -0.5 2,722,227 + yfiM b2586 0.24 -0.1 2,722,332 + yfiM b2586 0.56 -0.1 2,722,387 - yfiM b2586 0.73 +0.4 2,722,415 + yfiM b2586 0.82 -0.0 2,722,435 + yfiM b2586 0.88 -0.3 2,722,539 - -0.5 2,722,539 - +0.8 2,722,557 + -2.4 2,722,557 + +0.6 2,722,570 + +0.1 2,722,604 - kgtP b2587 0.10 -0.2 2,722,604 - kgtP b2587 0.10 +0.5 2,722,633 + kgtP b2587 0.13 +0.1 2,722,641 - kgtP b2587 0.13 -0.5 2,722,783 + kgtP b2587 0.24 +1.5 2,722,801 + kgtP b2587 0.25 +1.1 2,722,943 + kgtP b2587 0.36 +0.9 2,722,943 + kgtP b2587 0.36 +1.0 2,723,060 - kgtP b2587 0.45 -0.1 2,723,060 - kgtP b2587 0.45 +0.5 2,723,145 + kgtP b2587 0.52 +1.3 2,723,210 - kgtP b2587 0.57 +0.2 2,723,235 + kgtP b2587 0.59 +0.7 2,723,348 - kgtP b2587 0.68 -0.2 2,723,408 - kgtP b2587 0.72 +0.2 2,723,418 + kgtP b2587 0.73 +0.4 2,723,418 + kgtP b2587 0.73 +1.2 2,723,436 + kgtP b2587 0.74 -0.0 2,723,457 - kgtP b2587 0.76 +0.6 2,723,698 - -0.6
Or see this region's nucleotide sequence