Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegU and yegV overlap by 4 nucleotides yegV and yegW overlap by 27 nucleotides yegW and yegX are separated by 51 nucleotides yegX and thiD are separated by 64 nucleotides
b2099: yegU - predicted hydrolase (NCBI), at 2,178,117 to 2,179,121
yegU
b2100: yegV - predicted kinase (NCBI), at 2,179,118 to 2,180,083
yegV
b2101: yegW - predicted DNA-binding transcriptional regulator (NCBI), at 2,180,057 to 2,180,803
yegW
b2102: yegX - orf, hypothetical protein (VIMSS), at 2,180,855 to 2,181,673
yegX
b2103: thiD - phosphomethylpyrimidine kinase (NCBI), at 2,181,738 to 2,182,538
thiD
Position (kb)
2180
2181 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2179.214 kb on + strand at 2179.221 kb on - strand, within yegV at 2179.222 kb on - strand, within yegV at 2179.310 kb on + strand, within yegV at 2179.396 kb on - strand, within yegV at 2179.448 kb on - strand, within yegV at 2179.906 kb on - strand, within yegV at 2179.906 kb on - strand, within yegV at 2179.993 kb on - strand at 2180.103 kb on + strand at 2180.234 kb on + strand, within yegW at 2180.502 kb on - strand, within yegW at 2180.515 kb on + strand, within yegW at 2180.595 kb on - strand, within yegW at 2180.604 kb on + strand, within yegW at 2180.604 kb on + strand, within yegW at 2180.686 kb on - strand, within yegW at 2180.686 kb on - strand, within yegW at 2180.686 kb on - strand, within yegW at 2180.829 kb on + strand at 2180.837 kb on - strand at 2180.886 kb on + strand at 2180.896 kb on - strand at 2180.902 kb on - strand at 2180.902 kb on - strand at 2180.904 kb on - strand at 2180.917 kb on - strand at 2180.934 kb on + strand at 2180.934 kb on + strand at 2180.961 kb on - strand, within yegX at 2181.005 kb on - strand at 2181.005 kb on - strand, within yegX at 2181.027 kb on + strand, within yegX at 2181.027 kb on + strand, within yegX at 2181.202 kb on - strand, within yegX at 2181.202 kb on - strand, within yegX at 2181.221 kb on + strand, within yegX at 2181.256 kb on - strand, within yegX at 2181.376 kb on + strand, within yegX at 2181.460 kb on + strand, within yegX at 2181.467 kb on + strand, within yegX at 2181.485 kb on - strand, within yegX at 2181.497 kb on - strand, within yegX at 2181.516 kb on - strand, within yegX at 2181.525 kb on + strand, within yegX at 2181.548 kb on + strand, within yegX at 2181.628 kb on + strand at 2181.628 kb on + strand at 2181.631 kb on + strand at 2181.660 kb on + strand at 2181.678 kb on - strand at 2181.695 kb on + strand at 2181.726 kb on + strand at 2181.731 kb on - strand at 2181.749 kb on - strand at 2181.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 2,179,214 + +0.1 2,179,221 - yegV b2100 0.11 -0.1 2,179,222 - yegV b2100 0.11 -0.5 2,179,310 + yegV b2100 0.20 +0.6 2,179,396 - yegV b2100 0.29 -0.2 2,179,448 - yegV b2100 0.34 +0.1 2,179,906 - yegV b2100 0.82 +0.8 2,179,906 - yegV b2100 0.82 +0.1 2,179,993 - +0.1 2,180,103 + +2.6 2,180,234 + yegW b2101 0.24 +2.5 2,180,502 - yegW b2101 0.60 +3.2 2,180,515 + yegW b2101 0.61 +3.0 2,180,595 - yegW b2101 0.72 +2.9 2,180,604 + yegW b2101 0.73 +2.5 2,180,604 + yegW b2101 0.73 +3.5 2,180,686 - yegW b2101 0.84 +2.7 2,180,686 - yegW b2101 0.84 +3.1 2,180,686 - yegW b2101 0.84 +2.8 2,180,829 + +2.9 2,180,837 - -0.1 2,180,886 + +0.4 2,180,896 - -0.5 2,180,902 - -0.4 2,180,902 - -0.3 2,180,904 - +0.3 2,180,917 - +0.1 2,180,934 + +1.2 2,180,934 + +0.3 2,180,961 - yegX b2102 0.13 +0.4 2,181,005 - +0.1 2,181,005 - yegX b2102 0.18 -0.0 2,181,027 + yegX b2102 0.21 -1.6 2,181,027 + yegX b2102 0.21 +0.4 2,181,202 - yegX b2102 0.42 -0.1 2,181,202 - yegX b2102 0.42 -0.3 2,181,221 + yegX b2102 0.45 +0.3 2,181,256 - yegX b2102 0.49 -0.0 2,181,376 + yegX b2102 0.64 +0.5 2,181,460 + yegX b2102 0.74 -0.3 2,181,467 + yegX b2102 0.75 +0.3 2,181,485 - yegX b2102 0.77 -0.1 2,181,497 - yegX b2102 0.78 -0.1 2,181,516 - yegX b2102 0.81 -0.1 2,181,525 + yegX b2102 0.82 +0.3 2,181,548 + yegX b2102 0.85 -0.1 2,181,628 + -0.0 2,181,628 + -1.0 2,181,631 + -0.1 2,181,660 + +0.3 2,181,678 - +0.2 2,181,695 + +0.3 2,181,726 + -0.4 2,181,731 - +0.2 2,181,749 - +0.6 2,181,801 - +0.4
Or see this region's nucleotide sequence