Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobT and cobS are separated by 11 nucleotides cobS and cobU overlap by 4 nucleotides cobU and yeeH are separated by 303 nucleotides yeeH and insH-6 are separated by 150 nucleotides
b1991: cobT - nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (NCBI), at 2,061,412 to 2,062,491
cobT
b1992: cobS - cobalamin synthase (NCBI), at 2,062,503 to 2,063,246
cobS
b1993: cobU - adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (NCBI), at 2,063,243 to 2,063,788
cobU
b4639: yeeH - no description, at 2,064,092 to 2,064,178
yeeH
b1994: insH-6 - CP4-44 prophage; IS5 transposase and trans-activator (NCBI), at 2,064,329 to 2,065,345
insH-6
Position (kb)
2063
2064 Strain fitness (log2 ratio)
-2
-1
0
1 at 2062.333 kb on + strand, within cobT at 2062.333 kb on + strand, within cobT at 2062.357 kb on - strand, within cobT at 2062.416 kb on - strand at 2062.704 kb on + strand, within cobS at 2063.068 kb on + strand, within cobS at 2063.182 kb on + strand at 2063.182 kb on - strand at 2063.203 kb on - strand at 2063.378 kb on - strand, within cobU at 2063.378 kb on - strand, within cobU at 2063.390 kb on + strand, within cobU at 2063.527 kb on + strand, within cobU at 2063.705 kb on - strand, within cobU at 2063.710 kb on + strand, within cobU at 2063.728 kb on - strand, within cobU at 2063.728 kb on - strand, within cobU at 2063.728 kb on - strand, within cobU at 2063.728 kb on - strand, within cobU at 2063.764 kb on - strand at 2063.882 kb on - strand at 2063.882 kb on - strand at 2064.082 kb on - strand at 2064.082 kb on - strand at 2064.179 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 2,062,333 + cobT b1991 0.85 +0.8 2,062,333 + cobT b1991 0.85 +0.5 2,062,357 - cobT b1991 0.88 +0.4 2,062,416 - -0.6 2,062,704 + cobS b1992 0.27 +0.3 2,063,068 + cobS b1992 0.76 +0.4 2,063,182 + +0.2 2,063,182 - -0.2 2,063,203 - +0.8 2,063,378 - cobU b1993 0.25 -0.1 2,063,378 - cobU b1993 0.25 -2.0 2,063,390 + cobU b1993 0.27 +0.3 2,063,527 + cobU b1993 0.52 +0.0 2,063,705 - cobU b1993 0.85 -1.0 2,063,710 + cobU b1993 0.86 +0.1 2,063,728 - cobU b1993 0.89 -0.3 2,063,728 - cobU b1993 0.89 -1.1 2,063,728 - cobU b1993 0.89 -2.1 2,063,728 - cobU b1993 0.89 -1.3 2,063,764 - -0.3 2,063,882 - -1.2 2,063,882 - -0.8 2,064,082 - -2.2 2,064,082 - -1.5 2,064,179 - +0.4
Or see this region's nucleotide sequence