Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt intR and ydaQ are separated by 1 nucleotides ydaQ and ydaC are separated by 78 nucleotides ydaC and lar overlap by 8 nucleotides lar and recT are separated by 56 nucleotides recT and recE overlap by 8 nucleotides
b1345: intR - Rac prophage; integrase (NCBI), at 1,410,024 to 1,411,259
intR
b1346: ydaQ - orf, hypothetical protein (VIMSS), at 1,411,261 to 1,411,476
ydaQ
b1347: ydaC - Rac prophage; predicted protein (NCBI), at 1,411,555 to 1,411,764
ydaC
b1348: lar - Rac prophage; restriction alleviation protein (NCBI), at 1,411,757 to 1,411,951
lar
b1349: recT - Rac prophage; recombination and repair protein (NCBI), at 1,412,008 to 1,412,817
recT
b1350: recE - Rac prophage; exonuclease VIII, 5' -> 3' specific dsDNA exonuclease (NCBI), at 1,412,810 to 1,415,410
recE
Position (kb)
1412
1413 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1411.223 kb on - strand at 1411.308 kb on - strand, within ydaQ at 1411.308 kb on - strand, within ydaQ at 1411.364 kb on + strand, within ydaQ at 1411.483 kb on + strand at 1411.483 kb on + strand at 1411.534 kb on + strand at 1411.637 kb on - strand, within ydaC at 1411.696 kb on - strand, within ydaC at 1411.844 kb on - strand, within lar at 1411.855 kb on - strand, within lar at 1411.865 kb on - strand, within lar at 1411.865 kb on - strand, within lar at 1411.867 kb on - strand, within lar at 1411.880 kb on - strand, within lar at 1411.880 kb on - strand, within lar at 1412.066 kb on - strand at 1412.069 kb on - strand at 1412.069 kb on - strand at 1412.080 kb on - strand at 1412.090 kb on - strand, within recT at 1412.103 kb on - strand, within recT at 1412.103 kb on - strand, within recT at 1412.113 kb on - strand, within recT at 1412.192 kb on - strand, within recT at 1412.213 kb on - strand, within recT at 1412.213 kb on - strand, within recT at 1412.235 kb on - strand, within recT at 1412.302 kb on - strand, within recT at 1412.302 kb on - strand, within recT at 1412.538 kb on - strand, within recT at 1412.896 kb on + strand at 1412.896 kb on + strand at 1412.896 kb on - strand at 1412.896 kb on - strand at 1413.042 kb on + strand at 1413.054 kb on + strand at 1413.054 kb on + strand at 1413.135 kb on + strand, within recE at 1413.341 kb on + strand, within recE at 1413.411 kb on + strand, within recE at 1413.411 kb on + strand, within recE at 1413.755 kb on - strand, within recE at 1413.755 kb on - strand, within recE
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 1,411,223 - +1.0 1,411,308 - ydaQ b1346 0.22 -0.6 1,411,308 - ydaQ b1346 0.22 +2.5 1,411,364 + ydaQ b1346 0.48 -1.2 1,411,483 + +1.2 1,411,483 + -0.6 1,411,534 + -0.6 1,411,637 - ydaC b1347 0.39 +0.2 1,411,696 - ydaC b1347 0.67 +2.2 1,411,844 - lar b1348 0.45 +1.2 1,411,855 - lar b1348 0.50 +1.0 1,411,865 - lar b1348 0.55 +0.5 1,411,865 - lar b1348 0.55 -0.4 1,411,867 - lar b1348 0.56 +0.9 1,411,880 - lar b1348 0.63 +1.3 1,411,880 - lar b1348 0.63 +0.5 1,412,066 - +0.5 1,412,069 - +0.6 1,412,069 - +0.8 1,412,080 - +0.6 1,412,090 - recT b1349 0.10 +1.0 1,412,103 - recT b1349 0.12 +0.9 1,412,103 - recT b1349 0.12 +1.4 1,412,113 - recT b1349 0.13 +0.7 1,412,192 - recT b1349 0.23 +1.3 1,412,213 - recT b1349 0.25 +0.4 1,412,213 - recT b1349 0.25 +1.1 1,412,235 - recT b1349 0.28 +0.9 1,412,302 - recT b1349 0.36 +0.5 1,412,302 - recT b1349 0.36 +1.1 1,412,538 - recT b1349 0.65 -0.1 1,412,896 + +0.2 1,412,896 + +0.2 1,412,896 - +0.9 1,412,896 - +1.1 1,413,042 + +0.6 1,413,054 + +0.9 1,413,054 + -0.1 1,413,135 + recE b1350 0.12 -0.2 1,413,341 + recE b1350 0.20 -1.9 1,413,411 + recE b1350 0.23 +0.4 1,413,411 + recE b1350 0.23 +0.7 1,413,755 - recE b1350 0.36 +0.5 1,413,755 - recE b1350 0.36 +0.8
Or see this region's nucleotide sequence