Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt abgR and isrA are separated by 2 nucleotides isrA and ydaL are separated by 169 nucleotides ydaL and b1341 are separated by 20 nucleotides b1341 and ydaN are separated by 254 nucleotides
b1339: abgR - predicted DNA-binding transcriptional regulator (NCBI), at 1,402,765 to 1,403,673
abgR
b4426: isrA - unknown RNA (NCBI), at 1,403,676 to 1,403,833
isrA
b1340: ydaL - hypothetical protein (NCBI), at 1,404,003 to 1,404,566
ydaL
b1341: b1341 - orf, hypothetical protein (VIMSS), at 1,404,587 to 1,405,819
b1341
b1342: ydaN - zinc transporter (NCBI), at 1,406,074 to 1,407,057
ydaN
Position (kb)
1404
1405
1406 Strain fitness (log2 ratio)
-1
0
1
2 at 1403.720 kb on + strand, within isrA at 1403.720 kb on + strand, within isrA at 1403.732 kb on - strand, within isrA at 1403.735 kb on - strand, within isrA at 1404.417 kb on - strand, within ydaL at 1404.648 kb on + strand at 1404.666 kb on - strand at 1404.686 kb on - strand at 1404.900 kb on + strand, within b1341 at 1404.900 kb on + strand, within b1341 at 1404.929 kb on - strand, within b1341 at 1405.161 kb on - strand, within b1341 at 1405.163 kb on + strand, within b1341 at 1405.163 kb on + strand, within b1341 at 1405.166 kb on - strand, within b1341 at 1405.236 kb on + strand, within b1341 at 1405.236 kb on + strand, within b1341 at 1405.293 kb on + strand, within b1341 at 1405.495 kb on + strand, within b1341 at 1405.559 kb on + strand, within b1341 at 1405.665 kb on + strand, within b1341 at 1405.673 kb on - strand, within b1341 at 1405.676 kb on - strand, within b1341 at 1405.755 kb on - strand at 1405.767 kb on + strand at 1405.767 kb on + strand at 1405.776 kb on + strand at 1405.779 kb on - strand at 1405.785 kb on + strand at 1405.798 kb on - strand at 1405.877 kb on + strand at 1405.885 kb on - strand at 1405.885 kb on - strand at 1405.941 kb on - strand at 1405.941 kb on - strand at 1406.243 kb on + strand, within ydaN at 1406.396 kb on + strand, within ydaN at 1406.441 kb on + strand, within ydaN at 1406.441 kb on + strand, within ydaN at 1406.471 kb on + strand, within ydaN at 1406.479 kb on - strand, within ydaN at 1406.521 kb on + strand, within ydaN at 1406.521 kb on + strand, within ydaN at 1406.622 kb on + strand, within ydaN at 1406.678 kb on - strand, within ydaN at 1406.727 kb on + strand, within ydaN at 1406.727 kb on + strand, within ydaN at 1406.783 kb on + strand, within ydaN at 1406.812 kb on - strand, within ydaN at 1406.814 kb on - strand, within ydaN
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 1,403,720 + isrA b4426 0.28 -1.6 1,403,720 + isrA b4426 0.28 -0.6 1,403,732 - isrA b4426 0.35 -0.1 1,403,735 - isrA b4426 0.37 -0.3 1,404,417 - ydaL b1340 0.73 +0.5 1,404,648 + +1.3 1,404,666 - +0.7 1,404,686 - +1.3 1,404,900 + b1341 b1341 0.25 +1.1 1,404,900 + b1341 b1341 0.25 +1.6 1,404,929 - b1341 b1341 0.28 +1.1 1,405,161 - b1341 b1341 0.47 +1.0 1,405,163 + b1341 b1341 0.47 +1.3 1,405,163 + b1341 b1341 0.47 +0.8 1,405,166 - b1341 b1341 0.47 +0.6 1,405,236 + b1341 b1341 0.53 +0.9 1,405,236 + b1341 b1341 0.53 +2.2 1,405,293 + b1341 b1341 0.57 +1.2 1,405,495 + b1341 b1341 0.74 +0.7 1,405,559 + b1341 b1341 0.79 +1.0 1,405,665 + b1341 b1341 0.87 +1.7 1,405,673 - b1341 b1341 0.88 +1.3 1,405,676 - b1341 b1341 0.88 +0.7 1,405,755 - +1.4 1,405,767 + +0.3 1,405,767 + +0.5 1,405,776 + +1.1 1,405,779 - +1.1 1,405,785 + +1.3 1,405,798 - +1.1 1,405,877 + +1.1 1,405,885 - +0.0 1,405,885 - +1.2 1,405,941 - +0.2 1,405,941 - -1.5 1,406,243 + ydaN b1342 0.17 -0.1 1,406,396 + ydaN b1342 0.33 +0.3 1,406,441 + ydaN b1342 0.37 +0.4 1,406,441 + ydaN b1342 0.37 +0.7 1,406,471 + ydaN b1342 0.40 -0.4 1,406,479 - ydaN b1342 0.41 +0.9 1,406,521 + ydaN b1342 0.45 -0.1 1,406,521 + ydaN b1342 0.45 -0.4 1,406,622 + ydaN b1342 0.56 -0.1 1,406,678 - ydaN b1342 0.61 -0.1 1,406,727 + ydaN b1342 0.66 +0.3 1,406,727 + ydaN b1342 0.66 +0.2 1,406,783 + ydaN b1342 0.72 -0.1 1,406,812 - ydaN b1342 0.75 -0.0 1,406,814 - ydaN b1342 0.75 +0.9
Or see this region's nucleotide sequence