Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgB and dadA are separated by 329 nucleotides dadA and dadX are separated by 9 nucleotides
b1188: ycgB - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) , at 1,234,932 to 1,236,464
ycgB
b1189: dadA - D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data) , at 1,236,794 to 1,238,092
dadA
b1190: dadX - alanine racemase (NCBI), at 1,238,102 to 1,239,172
dadX
Position (kb)
1236
1237
1238
1239 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1235.815 kb on - strand, within ycgB at 1235.815 kb on - strand, within ycgB at 1236.122 kb on + strand, within ycgB at 1236.187 kb on - strand, within ycgB at 1236.339 kb on - strand at 1236.345 kb on - strand at 1236.374 kb on + strand at 1236.374 kb on + strand at 1236.414 kb on - strand at 1236.456 kb on - strand at 1236.788 kb on - strand at 1236.788 kb on - strand at 1236.803 kb on + strand at 1236.924 kb on - strand, within dadA at 1237.037 kb on + strand, within dadA at 1237.040 kb on - strand, within dadA at 1237.146 kb on + strand, within dadA at 1237.180 kb on + strand, within dadA at 1237.213 kb on + strand, within dadA at 1237.213 kb on + strand, within dadA at 1237.218 kb on + strand, within dadA at 1237.507 kb on + strand, within dadA at 1237.545 kb on - strand, within dadA at 1237.545 kb on - strand, within dadA at 1237.803 kb on + strand, within dadA at 1237.803 kb on + strand, within dadA at 1238.119 kb on + strand at 1238.119 kb on + strand at 1238.234 kb on - strand, within dadX at 1238.234 kb on - strand, within dadX at 1238.275 kb on + strand, within dadX at 1238.275 kb on + strand, within dadX at 1238.292 kb on + strand, within dadX at 1238.803 kb on + strand, within dadX at 1238.803 kb on + strand, within dadX at 1238.855 kb on + strand, within dadX at 1238.874 kb on - strand, within dadX at 1238.920 kb on - strand, within dadX at 1238.920 kb on - strand, within dadX
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 1,235,815 - ycgB b1188 0.58 -4.8 1,235,815 - ycgB b1188 0.58 -2.0 1,236,122 + ycgB b1188 0.78 -1.8 1,236,187 - ycgB b1188 0.82 -2.2 1,236,339 - -3.0 1,236,345 - -2.3 1,236,374 + -4.4 1,236,374 + -2.8 1,236,414 - -1.3 1,236,456 - -2.1 1,236,788 - -0.0 1,236,788 - +0.4 1,236,803 + -0.5 1,236,924 - dadA b1189 0.10 +0.0 1,237,037 + dadA b1189 0.19 +0.3 1,237,040 - dadA b1189 0.19 +0.6 1,237,146 + dadA b1189 0.27 +0.4 1,237,180 + dadA b1189 0.30 +0.3 1,237,213 + dadA b1189 0.32 -0.2 1,237,213 + dadA b1189 0.32 +1.4 1,237,218 + dadA b1189 0.33 +0.4 1,237,507 + dadA b1189 0.55 +0.1 1,237,545 - dadA b1189 0.58 +0.3 1,237,545 - dadA b1189 0.58 +1.4 1,237,803 + dadA b1189 0.78 +0.3 1,237,803 + dadA b1189 0.78 +0.6 1,238,119 + +2.5 1,238,119 + +2.1 1,238,234 - dadX b1190 0.12 +1.8 1,238,234 - dadX b1190 0.12 +2.3 1,238,275 + dadX b1190 0.16 +2.1 1,238,275 + dadX b1190 0.16 +2.4 1,238,292 + dadX b1190 0.18 +2.1 1,238,803 + dadX b1190 0.65 +2.4 1,238,803 + dadX b1190 0.65 +2.5 1,238,855 + dadX b1190 0.70 +1.9 1,238,874 - dadX b1190 0.72 +2.6 1,238,920 - dadX b1190 0.76 +2.6 1,238,920 - dadX b1190 0.76 +3.9
Or see this region's nucleotide sequence