Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiY and mipB are separated by 130 nucleotides mipB and moeB are separated by 75 nucleotides moeB and moeA overlap by 1 nucleotides
b0824: ybiY - predicted pyruvate formate lyase activating enzyme (RefSeq), at 861,835 to 862,734
ybiY
b0825: mipB - putative transaldolase (VIMSS), at 862,865 to 863,527
mipB
b0826: moeB - molybdopterin biosynthesis protein MoeB (NCBI), at 863,603 to 864,352
moeB
b0827: moeA - molybdopterin biosynthesis protein (NCBI), at 864,352 to 865,587
moeA
Position (kb) 862
863
864 Strain fitness (log2 ratio) -1
0
1
2 at 861.947 kb on - strand, within ybiY at 862.001 kb on + strand, within ybiY at 862.063 kb on - strand, within ybiY at 862.219 kb on - strand, within ybiY at 862.295 kb on + strand, within ybiY at 862.398 kb on - strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.439 kb on - strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.462 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.580 kb on + strand, within ybiY at 862.600 kb on + strand, within ybiY at 862.697 kb on - strand at 862.717 kb on - strand at 862.828 kb on + strand at 863.057 kb on + strand, within mipB at 863.251 kb on + strand, within mipB at 863.433 kb on + strand, within mipB at 863.554 kb on + strand at 863.625 kb on - strand at 863.673 kb on - strand at 863.881 kb on + strand, within moeB at 863.881 kb on + strand, within moeB at 863.980 kb on - strand, within moeB at 863.987 kb on + strand, within moeB at 864.031 kb on - strand, within moeB at 864.031 kb on - strand, within moeB at 864.040 kb on - strand, within moeB at 864.067 kb on + strand, within moeB at 864.071 kb on - strand, within moeB at 864.129 kb on + strand, within moeB at 864.167 kb on + strand, within moeB at 864.209 kb on - strand, within moeB at 864.209 kb on - strand, within moeB at 864.214 kb on - strand, within moeB at 864.271 kb on - strand, within moeB
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 861,947 - ybiY b0824 0.12 -0.7 862,001 + ybiY b0824 0.18 +0.0 862,063 - ybiY b0824 0.25 -0.2 862,219 - ybiY b0824 0.43 -0.4 862,295 + ybiY b0824 0.51 +0.3 862,398 - ybiY b0824 0.63 +1.2 862,435 + ybiY b0824 0.67 -0.1 862,435 + ybiY b0824 0.67 -0.0 862,439 - ybiY b0824 0.67 -0.8 862,442 + ybiY b0824 0.67 +0.1 862,442 + ybiY b0824 0.67 +0.3 862,462 + ybiY b0824 0.70 -0.2 862,575 + ybiY b0824 0.82 -0.2 862,575 + ybiY b0824 0.82 +0.2 862,580 + ybiY b0824 0.83 -0.5 862,600 + ybiY b0824 0.85 -0.5 862,697 - -0.5 862,717 - -0.4 862,828 + +1.9 863,057 + mipB b0825 0.29 +0.3 863,251 + mipB b0825 0.58 -0.0 863,433 + mipB b0825 0.86 -0.2 863,554 + -0.3 863,625 - -1.1 863,673 - -0.1 863,881 + moeB b0826 0.37 +0.4 863,881 + moeB b0826 0.37 -0.6 863,980 - moeB b0826 0.50 -0.8 863,987 + moeB b0826 0.51 +0.1 864,031 - moeB b0826 0.57 +0.7 864,031 - moeB b0826 0.57 +0.3 864,040 - moeB b0826 0.58 +0.1 864,067 + moeB b0826 0.62 -0.2 864,071 - moeB b0826 0.62 -0.1 864,129 + moeB b0826 0.70 +0.4 864,167 + moeB b0826 0.75 +0.3 864,209 - moeB b0826 0.81 +0.1 864,209 - moeB b0826 0.81 -0.2 864,214 - moeB b0826 0.81 +0.2 864,271 - moeB b0826 0.89 +0.1
Or see this region's nucleotide sequence