Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiW and ybiY are separated by 5 nucleotides ybiY and mipB are separated by 130 nucleotides mipB and moeB are separated by 75 nucleotides
b0823: ybiW - predicted pyruvate formate lyase (NCBI), at 859,397 to 861,829
ybiW
b0824: ybiY - predicted pyruvate formate lyase activating enzyme (RefSeq), at 861,835 to 862,734
ybiY
b0825: mipB - putative transaldolase (VIMSS), at 862,865 to 863,527
mipB
b0826: moeB - molybdopterin biosynthesis protein MoeB (NCBI), at 863,603 to 864,352
moeB
Position (kb) 861
862
863 Strain fitness (log2 ratio) -1
0
1
2 at 860.884 kb on - strand, within ybiW at 861.029 kb on - strand, within ybiW at 861.287 kb on - strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.715 kb on + strand at 861.715 kb on + strand at 861.723 kb on - strand at 861.728 kb on - strand at 861.749 kb on - strand at 861.947 kb on - strand, within ybiY at 862.001 kb on + strand, within ybiY at 862.063 kb on - strand, within ybiY at 862.219 kb on - strand, within ybiY at 862.295 kb on + strand, within ybiY at 862.398 kb on - strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.439 kb on - strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.462 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.580 kb on + strand, within ybiY at 862.600 kb on + strand, within ybiY at 862.697 kb on - strand at 862.717 kb on - strand at 862.828 kb on + strand at 863.057 kb on + strand, within mipB at 863.251 kb on + strand, within mipB at 863.433 kb on + strand, within mipB at 863.554 kb on + strand at 863.625 kb on - strand at 863.673 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 860,884 - ybiW b0823 0.61 -0.1 861,029 - ybiW b0823 0.67 -0.1 861,287 - ybiW b0823 0.78 +0.5 861,428 + ybiW b0823 0.83 -0.5 861,428 + ybiW b0823 0.83 +0.1 861,428 + ybiW b0823 0.83 -0.5 861,715 + -0.2 861,715 + -0.6 861,723 - -0.5 861,728 - -0.2 861,749 - -0.4 861,947 - ybiY b0824 0.12 -0.7 862,001 + ybiY b0824 0.18 +0.0 862,063 - ybiY b0824 0.25 -0.2 862,219 - ybiY b0824 0.43 -0.4 862,295 + ybiY b0824 0.51 +0.3 862,398 - ybiY b0824 0.63 +1.2 862,435 + ybiY b0824 0.67 -0.1 862,435 + ybiY b0824 0.67 -0.0 862,439 - ybiY b0824 0.67 -0.8 862,442 + ybiY b0824 0.67 +0.1 862,442 + ybiY b0824 0.67 +0.3 862,462 + ybiY b0824 0.70 -0.2 862,575 + ybiY b0824 0.82 -0.2 862,575 + ybiY b0824 0.82 +0.2 862,580 + ybiY b0824 0.83 -0.5 862,600 + ybiY b0824 0.85 -0.5 862,697 - -0.5 862,717 - -0.4 862,828 + +1.9 863,057 + mipB b0825 0.29 +0.3 863,251 + mipB b0825 0.58 -0.0 863,433 + mipB b0825 0.86 -0.2 863,554 + -0.3 863,625 - -1.1 863,673 - -0.1
Or see this region's nucleotide sequence