Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fur and uof overlap by 8 nucleotides uof and fldA are separated by 209 nucleotides fldA and ybfE are separated by 139 nucleotides ybfE and ybfF are separated by 139 nucleotides
b0683: fur - ferric uptake regulator (NCBI), at 709,423 to 709,869
fur
b4637: uof - ryhB-regulated fur leader peptide (RefSeq), at 709,862 to 709,948
uof
b0684: fldA - flavodoxin 1 (NCBI), at 710,158 to 710,688
fldA
b0685: ybfE - orf, hypothetical protein (VIMSS), at 710,828 to 711,121
ybfE
b0686: ybfF - hypothetical protein (NCBI), at 711,261 to 712,025
ybfF
Position (kb) 710
711
712 Strain fitness (log2 ratio) -1
0
1 at 709.861 kb on - strand at 710.067 kb on - strand at 710.080 kb on + strand at 710.148 kb on - strand at 710.148 kb on - strand at 710.163 kb on - strand at 710.751 kb on + strand at 710.780 kb on - strand at 710.780 kb on - strand at 710.787 kb on + strand at 710.798 kb on + strand at 710.805 kb on - strand at 710.805 kb on - strand at 710.807 kb on + strand at 710.901 kb on + strand, within ybfE at 710.924 kb on + strand, within ybfE at 710.924 kb on + strand, within ybfE at 711.159 kb on - strand at 711.400 kb on - strand, within ybfF at 711.569 kb on + strand, within ybfF at 711.668 kb on - strand, within ybfF at 711.685 kb on + strand, within ybfF at 711.728 kb on - strand, within ybfF at 711.730 kb on - strand, within ybfF at 711.730 kb on - strand, within ybfF at 711.803 kb on - strand, within ybfF at 711.803 kb on - strand, within ybfF at 711.900 kb on - strand, within ybfF at 711.923 kb on + strand, within ybfF at 711.923 kb on + strand, within ybfF at 711.933 kb on + strand, within ybfF at 712.060 kb on + strand at 712.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 709,861 - -0.5 710,067 - -0.8 710,080 + -0.2 710,148 - -0.2 710,148 - +0.6 710,163 - -0.4 710,751 + -0.3 710,780 - -0.8 710,780 - -0.4 710,787 + +0.6 710,798 + +0.1 710,805 - -0.8 710,805 - -1.2 710,807 + +0.1 710,901 + ybfE b0685 0.25 +0.4 710,924 + ybfE b0685 0.33 +1.0 710,924 + ybfE b0685 0.33 +0.5 711,159 - -0.3 711,400 - ybfF b0686 0.18 +0.2 711,569 + ybfF b0686 0.40 +0.0 711,668 - ybfF b0686 0.53 +0.3 711,685 + ybfF b0686 0.55 -0.2 711,728 - ybfF b0686 0.61 +0.7 711,730 - ybfF b0686 0.61 -0.3 711,730 - ybfF b0686 0.61 -1.5 711,803 - ybfF b0686 0.71 -0.8 711,803 - ybfF b0686 0.71 +0.4 711,900 - ybfF b0686 0.84 -0.4 711,923 + ybfF b0686 0.87 -0.0 711,923 + ybfF b0686 0.87 +0.6 711,933 + ybfF b0686 0.88 +0.6 712,060 + -0.1 712,077 + -0.1
Or see this region's nucleotide sequence