Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fepE and fepC overlap by 4 nucleotides fepC and fepG overlap by 4 nucleotides fepG and fepD overlap by 4 nucleotides
b0587: fepE - regulator of length of O-antigen component of lipopolysaccharide chains (NCBI), at 617,477 to 618,610
fepE
b0588: fepC - iron-enterobactin transporter subunit (NCBI), at 618,607 to 619,422
fepC
b0589: fepG - iron-enterobactin transporter subunit (NCBI), at 619,419 to 620,411
fepG
b0590: fepD - iron-enterobactin transporter subunit (NCBI), at 620,408 to 621,412
fepD
Position (kb) 618
619
620 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 617.616 kb on + strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.644 kb on - strand, within fepE at 617.701 kb on - strand, within fepE at 617.755 kb on - strand, within fepE at 617.849 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.893 kb on + strand, within fepE at 617.947 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.985 kb on - strand, within fepE at 617.986 kb on - strand, within fepE at 618.013 kb on + strand, within fepE at 618.013 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.018 kb on + strand, within fepE at 618.029 kb on - strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.035 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.045 kb on + strand, within fepE at 618.053 kb on - strand, within fepE at 618.071 kb on + strand, within fepE at 618.082 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.104 kb on + strand, within fepE at 618.114 kb on + strand, within fepE at 618.120 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.151 kb on - strand, within fepE at 618.190 kb on + strand, within fepE at 618.250 kb on - strand, within fepE at 618.267 kb on - strand, within fepE at 618.271 kb on - strand, within fepE at 618.300 kb on - strand, within fepE at 618.305 kb on - strand, within fepE at 618.361 kb on - strand, within fepE at 618.371 kb on - strand, within fepE at 618.377 kb on + strand, within fepE at 618.377 kb on + strand, within fepE at 618.383 kb on - strand, within fepE at 618.386 kb on - strand, within fepE at 618.412 kb on + strand, within fepE at 618.412 kb on + strand, within fepE at 618.412 kb on + strand, within fepE at 618.418 kb on - strand, within fepE at 618.457 kb on + strand, within fepE at 618.484 kb on + strand, within fepE at 618.498 kb on + strand at 618.500 kb on - strand at 618.500 kb on - strand at 618.520 kb on - strand at 618.545 kb on - strand at 618.561 kb on - strand at 618.561 kb on - strand at 618.598 kb on - strand at 618.721 kb on + strand, within fepC at 618.725 kb on - strand, within fepC at 618.815 kb on - strand, within fepC at 618.815 kb on - strand, within fepC at 618.876 kb on - strand, within fepC at 618.905 kb on - strand, within fepC at 618.927 kb on - strand, within fepC at 618.935 kb on - strand, within fepC at 618.935 kb on - strand, within fepC at 618.970 kb on - strand, within fepC at 619.003 kb on + strand, within fepC at 619.038 kb on + strand, within fepC at 619.066 kb on - strand, within fepC at 619.131 kb on - strand, within fepC at 619.259 kb on - strand, within fepC at 619.259 kb on - strand, within fepC at 619.261 kb on + strand, within fepC at 619.375 kb on + strand at 619.568 kb on - strand, within fepG at 619.568 kb on - strand, within fepG at 619.680 kb on - strand, within fepG at 619.685 kb on - strand, within fepG at 619.685 kb on - strand, within fepG at 619.750 kb on - strand, within fepG at 619.750 kb on - strand, within fepG at 619.808 kb on + strand, within fepG at 619.812 kb on + strand, within fepG at 619.827 kb on - strand, within fepG at 619.870 kb on - strand, within fepG at 619.872 kb on + strand, within fepG at 619.948 kb on - strand, within fepG at 620.042 kb on - strand, within fepG at 620.050 kb on - strand, within fepG at 620.050 kb on - strand, within fepG at 620.203 kb on - strand, within fepG at 620.205 kb on + strand, within fepG at 620.205 kb on + strand, within fepG at 620.263 kb on - strand, within fepG at 620.336 kb on - strand at 620.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 617,616 + fepE b0587 0.12 +2.5 617,644 - fepE b0587 0.15 +0.2 617,644 - fepE b0587 0.15 -0.4 617,644 - fepE b0587 0.15 +1.0 617,701 - fepE b0587 0.20 -0.9 617,755 - fepE b0587 0.25 -0.3 617,849 + fepE b0587 0.33 -0.3 617,893 + fepE b0587 0.37 +1.0 617,893 + fepE b0587 0.37 -1.4 617,947 - fepE b0587 0.41 +2.5 617,985 - fepE b0587 0.45 +1.5 617,985 - fepE b0587 0.45 +0.9 617,986 - fepE b0587 0.45 -0.6 618,013 + fepE b0587 0.47 +0.3 618,013 + fepE b0587 0.47 -0.1 618,018 + fepE b0587 0.48 +1.5 618,018 + fepE b0587 0.48 -0.1 618,029 - fepE b0587 0.49 +0.5 618,035 + fepE b0587 0.49 +1.2 618,035 + fepE b0587 0.49 +0.7 618,035 + fepE b0587 0.49 +0.9 618,045 + fepE b0587 0.50 +0.0 618,045 + fepE b0587 0.50 -0.3 618,053 - fepE b0587 0.51 +2.5 618,071 + fepE b0587 0.52 -0.8 618,082 + fepE b0587 0.53 +0.2 618,104 + fepE b0587 0.55 -0.7 618,104 + fepE b0587 0.55 +2.5 618,114 + fepE b0587 0.56 +0.5 618,120 - fepE b0587 0.57 +1.5 618,151 - fepE b0587 0.59 +0.2 618,151 - fepE b0587 0.59 +1.9 618,190 + fepE b0587 0.63 -0.4 618,250 - fepE b0587 0.68 -1.5 618,267 - fepE b0587 0.70 +1.5 618,271 - fepE b0587 0.70 +0.1 618,300 - fepE b0587 0.73 +0.6 618,305 - fepE b0587 0.73 +0.4 618,361 - fepE b0587 0.78 +1.2 618,371 - fepE b0587 0.79 +0.2 618,377 + fepE b0587 0.79 +0.9 618,377 + fepE b0587 0.79 +0.3 618,383 - fepE b0587 0.80 +0.0 618,386 - fepE b0587 0.80 +0.7 618,412 + fepE b0587 0.82 +0.5 618,412 + fepE b0587 0.82 +0.4 618,412 + fepE b0587 0.82 +1.2 618,418 - fepE b0587 0.83 -0.3 618,457 + fepE b0587 0.86 -0.6 618,484 + fepE b0587 0.89 +0.2 618,498 + +0.4 618,500 - +0.1 618,500 - +0.4 618,520 - +0.0 618,545 - +0.3 618,561 - -0.1 618,561 - +0.5 618,598 - +1.0 618,721 + fepC b0588 0.14 -2.8 618,725 - fepC b0588 0.14 -3.4 618,815 - fepC b0588 0.25 -2.1 618,815 - fepC b0588 0.25 -4.4 618,876 - fepC b0588 0.33 -2.3 618,905 - fepC b0588 0.37 -3.3 618,927 - fepC b0588 0.39 -1.9 618,935 - fepC b0588 0.40 -3.4 618,935 - fepC b0588 0.40 -1.3 618,970 - fepC b0588 0.44 -2.4 619,003 + fepC b0588 0.49 -1.7 619,038 + fepC b0588 0.53 -3.5 619,066 - fepC b0588 0.56 -3.2 619,131 - fepC b0588 0.64 -2.4 619,259 - fepC b0588 0.80 -3.6 619,259 - fepC b0588 0.80 -2.7 619,261 + fepC b0588 0.80 -4.5 619,375 + -4.6 619,568 - fepG b0589 0.15 -3.1 619,568 - fepG b0589 0.15 -1.8 619,680 - fepG b0589 0.26 -3.1 619,685 - fepG b0589 0.27 -0.0 619,685 - fepG b0589 0.27 -4.8 619,750 - fepG b0589 0.33 -2.6 619,750 - fepG b0589 0.33 -2.5 619,808 + fepG b0589 0.39 -3.4 619,812 + fepG b0589 0.40 -2.4 619,827 - fepG b0589 0.41 -1.5 619,870 - fepG b0589 0.45 -4.1 619,872 + fepG b0589 0.46 -0.8 619,948 - fepG b0589 0.53 -2.1 620,042 - fepG b0589 0.63 -2.5 620,050 - fepG b0589 0.64 -3.8 620,050 - fepG b0589 0.64 -1.7 620,203 - fepG b0589 0.79 -1.7 620,205 + fepG b0589 0.79 -2.2 620,205 + fepG b0589 0.79 -2.9 620,263 - fepG b0589 0.85 -3.1 620,336 - -3.9 620,392 - -0.9
Or see this region's nucleotide sequence