Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aes and gsk are separated by 151 nucleotides gsk and ybaL are separated by 132 nucleotides
b0476: aes - acetyl esterase (NCBI), at 498,238 to 499,197
aes
b0477: gsk - inosine/guanosine kinase (NCBI), at 499,349 to 500,653
gsk
b0478: ybaL - predicted transporter with NAD(P)-binding Rossmann-fold domain (NCBI), at 500,786 to 502,462
ybaL
Position (kb) 499
500
501 Strain fitness (log2 ratio) -1
0
1 at 498.393 kb on + strand, within aes at 498.393 kb on + strand, within aes at 498.461 kb on + strand, within aes at 498.464 kb on + strand, within aes at 498.476 kb on + strand, within aes at 498.490 kb on + strand, within aes at 498.531 kb on - strand, within aes at 498.531 kb on - strand, within aes at 498.546 kb on + strand, within aes at 498.546 kb on + strand, within aes at 498.564 kb on - strand, within aes at 498.564 kb on - strand, within aes at 498.624 kb on - strand, within aes at 498.675 kb on - strand, within aes at 498.730 kb on - strand, within aes at 498.740 kb on + strand, within aes at 498.839 kb on - strand, within aes at 498.894 kb on - strand, within aes at 498.942 kb on + strand, within aes at 498.960 kb on - strand, within aes at 499.132 kb on + strand at 499.138 kb on - strand at 499.285 kb on - strand at 499.453 kb on + strand at 499.457 kb on - strand at 499.526 kb on - strand, within gsk at 499.550 kb on + strand, within gsk at 499.558 kb on + strand, within gsk at 499.631 kb on + strand, within gsk at 499.631 kb on + strand, within gsk at 499.639 kb on + strand, within gsk at 499.656 kb on - strand, within gsk at 499.656 kb on - strand, within gsk at 499.656 kb on - strand, within gsk at 499.803 kb on + strand, within gsk at 499.803 kb on + strand, within gsk at 499.856 kb on - strand, within gsk at 499.913 kb on + strand, within gsk at 499.913 kb on + strand, within gsk at 499.951 kb on + strand, within gsk at 499.952 kb on + strand, within gsk at 500.002 kb on + strand, within gsk at 500.027 kb on - strand, within gsk at 500.072 kb on - strand, within gsk at 500.168 kb on + strand, within gsk at 500.176 kb on - strand, within gsk at 500.186 kb on - strand, within gsk at 500.202 kb on - strand, within gsk at 500.202 kb on - strand, within gsk at 500.302 kb on + strand, within gsk at 500.306 kb on + strand, within gsk at 500.359 kb on + strand, within gsk at 500.404 kb on + strand, within gsk at 500.471 kb on - strand, within gsk at 500.581 kb on + strand at 500.581 kb on + strand at 500.633 kb on - strand at 500.633 kb on - strand at 500.643 kb on - strand at 500.664 kb on + strand at 500.692 kb on - strand at 500.794 kb on + strand at 500.817 kb on + strand at 500.868 kb on + strand at 500.868 kb on + strand at 501.023 kb on + strand, within ybaL at 501.023 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.065 kb on + strand, within ybaL at 501.073 kb on - strand, within ybaL at 501.073 kb on - strand, within ybaL at 501.073 kb on - strand, within ybaL at 501.075 kb on + strand, within ybaL at 501.178 kb on + strand, within ybaL at 501.184 kb on - strand, within ybaL at 501.184 kb on - strand, within ybaL at 501.316 kb on + strand, within ybaL at 501.316 kb on + strand, within ybaL at 501.324 kb on - strand, within ybaL at 501.358 kb on - strand, within ybaL at 501.358 kb on - strand, within ybaL at 501.417 kb on + strand, within ybaL at 501.417 kb on + strand, within ybaL at 501.455 kb on + strand, within ybaL at 501.455 kb on + strand, within ybaL at 501.468 kb on + strand, within ybaL
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 498,393 + aes b0476 0.16 +0.6 498,393 + aes b0476 0.16 +0.8 498,461 + aes b0476 0.23 -0.2 498,464 + aes b0476 0.24 +0.1 498,476 + aes b0476 0.25 -0.2 498,490 + aes b0476 0.26 -1.3 498,531 - aes b0476 0.31 +0.5 498,531 - aes b0476 0.31 +0.7 498,546 + aes b0476 0.32 -0.1 498,546 + aes b0476 0.32 -0.2 498,564 - aes b0476 0.34 -0.8 498,564 - aes b0476 0.34 -0.4 498,624 - aes b0476 0.40 +0.2 498,675 - aes b0476 0.46 -0.6 498,730 - aes b0476 0.51 +0.3 498,740 + aes b0476 0.52 -0.4 498,839 - aes b0476 0.63 -0.0 498,894 - aes b0476 0.68 +0.8 498,942 + aes b0476 0.73 -0.0 498,960 - aes b0476 0.75 +0.3 499,132 + +0.1 499,138 - -0.2 499,285 - +0.6 499,453 + -0.2 499,457 - +0.2 499,526 - gsk b0477 0.14 +0.7 499,550 + gsk b0477 0.15 -0.1 499,558 + gsk b0477 0.16 -0.5 499,631 + gsk b0477 0.22 -0.0 499,631 + gsk b0477 0.22 -0.3 499,639 + gsk b0477 0.22 -0.9 499,656 - gsk b0477 0.24 -0.7 499,656 - gsk b0477 0.24 -0.3 499,656 - gsk b0477 0.24 -0.7 499,803 + gsk b0477 0.35 -0.1 499,803 + gsk b0477 0.35 +0.4 499,856 - gsk b0477 0.39 +0.4 499,913 + gsk b0477 0.43 -0.5 499,913 + gsk b0477 0.43 +1.0 499,951 + gsk b0477 0.46 -0.5 499,952 + gsk b0477 0.46 -0.5 500,002 + gsk b0477 0.50 +0.2 500,027 - gsk b0477 0.52 -0.2 500,072 - gsk b0477 0.55 +1.2 500,168 + gsk b0477 0.63 +0.3 500,176 - gsk b0477 0.63 -0.9 500,186 - gsk b0477 0.64 +0.3 500,202 - gsk b0477 0.65 -0.0 500,202 - gsk b0477 0.65 -0.1 500,302 + gsk b0477 0.73 -0.0 500,306 + gsk b0477 0.73 +0.1 500,359 + gsk b0477 0.77 +0.5 500,404 + gsk b0477 0.81 -0.0 500,471 - gsk b0477 0.86 -0.3 500,581 + +0.5 500,581 + -0.6 500,633 - -0.6 500,633 - -0.3 500,643 - +0.1 500,664 + +0.0 500,692 - +0.8 500,794 + +0.1 500,817 + -0.2 500,868 + +1.1 500,868 + +0.1 501,023 + ybaL b0478 0.14 -0.5 501,023 + ybaL b0478 0.14 +1.2 501,065 + ybaL b0478 0.17 -0.1 501,065 + ybaL b0478 0.17 -0.9 501,065 + ybaL b0478 0.17 +1.1 501,065 + ybaL b0478 0.17 +0.1 501,065 + ybaL b0478 0.17 -1.1 501,065 + ybaL b0478 0.17 -0.1 501,065 + ybaL b0478 0.17 +0.3 501,073 - ybaL b0478 0.17 +0.0 501,073 - ybaL b0478 0.17 +0.5 501,073 - ybaL b0478 0.17 +0.4 501,075 + ybaL b0478 0.17 -0.3 501,178 + ybaL b0478 0.23 +0.3 501,184 - ybaL b0478 0.24 +0.2 501,184 - ybaL b0478 0.24 -0.5 501,316 + ybaL b0478 0.32 +0.5 501,316 + ybaL b0478 0.32 +0.8 501,324 - ybaL b0478 0.32 -0.4 501,358 - ybaL b0478 0.34 -0.2 501,358 - ybaL b0478 0.34 +0.4 501,417 + ybaL b0478 0.38 +0.9 501,417 + ybaL b0478 0.38 +0.4 501,455 + ybaL b0478 0.40 -0.7 501,455 + ybaL b0478 0.40 -0.7 501,468 + ybaL b0478 0.41 +0.8
Or see this region's nucleotide sequence