Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgE and ykgF are separated by 10 nucleotides ykgF and ykgG overlap by 8 nucleotides ykgG and ykgH are separated by 242 nucleotides
b0306: ykgE - predicted oxidoreductase (NCBI), at 320,832 to 321,551
ykgE
b0307: ykgF - predicted amino acid dehydrogenase with NAD(P)-binding domain and ferridoxin-like domain (NCBI), at 321,562 to 322,989
ykgF
b0308: ykgG - putative transporter (VIMSS), at 322,982 to 323,677
ykgG
b0310: ykgH - predicted inner membrane protein (NCBI), at 323,920 to 324,588
ykgH
Position (kb) 321
322
323 Strain fitness (log2 ratio) -2
-1
0
1
2 at 320.576 kb on - strand at 320.654 kb on - strand at 320.723 kb on + strand at 320.728 kb on + strand at 320.729 kb on + strand at 320.729 kb on - strand at 320.731 kb on - strand at 320.781 kb on - strand at 320.788 kb on - strand at 320.788 kb on - strand at 320.807 kb on + strand at 320.813 kb on + strand at 320.815 kb on - strand at 320.821 kb on - strand at 320.865 kb on - strand at 320.883 kb on - strand at 320.884 kb on - strand at 320.886 kb on + strand at 320.886 kb on + strand at 320.894 kb on + strand at 320.894 kb on + strand at 320.898 kb on + strand at 320.898 kb on + strand at 320.899 kb on - strand at 320.901 kb on - strand at 320.904 kb on - strand, within ykgE at 320.944 kb on + strand, within ykgE at 320.944 kb on + strand, within ykgE at 320.959 kb on + strand, within ykgE at 320.959 kb on + strand, within ykgE at 320.971 kb on - strand, within ykgE at 320.975 kb on - strand, within ykgE at 320.975 kb on - strand, within ykgE at 320.977 kb on - strand, within ykgE at 320.978 kb on - strand, within ykgE at 320.983 kb on + strand, within ykgE at 321.022 kb on + strand, within ykgE at 321.027 kb on + strand, within ykgE at 321.027 kb on + strand, within ykgE at 321.035 kb on - strand, within ykgE at 321.065 kb on + strand, within ykgE at 321.073 kb on - strand, within ykgE at 321.106 kb on - strand, within ykgE at 321.202 kb on - strand, within ykgE at 321.221 kb on - strand, within ykgE at 321.221 kb on - strand, within ykgE at 321.236 kb on - strand, within ykgE at 321.236 kb on - strand, within ykgE at 321.249 kb on - strand, within ykgE at 321.266 kb on + strand, within ykgE at 321.307 kb on + strand, within ykgE at 321.321 kb on + strand, within ykgE at 321.325 kb on + strand, within ykgE at 321.325 kb on + strand, within ykgE at 321.335 kb on + strand, within ykgE at 321.436 kb on - strand, within ykgE at 321.461 kb on + strand, within ykgE at 321.461 kb on + strand, within ykgE at 321.533 kb on + strand at 321.533 kb on + strand at 321.552 kb on + strand at 321.553 kb on + strand at 321.557 kb on + strand at 321.672 kb on - strand at 321.699 kb on + strand at 321.732 kb on + strand, within ykgF at 321.732 kb on + strand, within ykgF at 321.736 kb on + strand, within ykgF at 321.750 kb on + strand, within ykgF at 321.918 kb on + strand, within ykgF at 321.960 kb on + strand, within ykgF at 321.960 kb on + strand, within ykgF at 322.013 kb on + strand, within ykgF at 322.028 kb on + strand, within ykgF at 322.028 kb on + strand, within ykgF at 322.034 kb on + strand, within ykgF at 322.075 kb on + strand, within ykgF at 322.081 kb on + strand, within ykgF at 322.154 kb on + strand, within ykgF at 322.239 kb on + strand, within ykgF at 322.477 kb on - strand, within ykgF at 322.516 kb on + strand, within ykgF at 322.516 kb on + strand, within ykgF at 322.560 kb on - strand, within ykgF at 322.641 kb on + strand, within ykgF at 322.660 kb on + strand, within ykgF at 323.074 kb on + strand, within ykgG at 323.074 kb on + strand, within ykgG at 323.094 kb on + strand, within ykgG at 323.105 kb on + strand, within ykgG at 323.168 kb on + strand, within ykgG at 323.237 kb on + strand, within ykgG at 323.307 kb on - strand, within ykgG at 323.346 kb on - strand, within ykgG at 323.346 kb on - strand, within ykgG at 323.359 kb on + strand, within ykgG at 323.359 kb on + strand, within ykgG at 323.500 kb on + strand, within ykgG at 323.503 kb on + strand, within ykgG at 323.503 kb on - strand, within ykgG at 323.583 kb on - strand, within ykgG at 323.612 kb on - strand at 323.614 kb on + strand at 323.614 kb on + strand at 323.621 kb on - strand at 323.623 kb on + strand at 323.623 kb on + strand at 323.623 kb on + strand at 323.628 kb on + strand at 323.628 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.651 kb on + strand at 323.690 kb on + strand at 323.696 kb on + strand at 323.698 kb on - strand at 323.701 kb on + strand at 323.701 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.742 kb on + strand at 323.748 kb on + strand at 323.748 kb on + strand at 323.748 kb on + strand at 323.749 kb on + strand at 323.757 kb on + strand at 323.757 kb on + strand at 323.759 kb on + strand at 323.759 kb on + strand at 323.759 kb on + strand at 323.874 kb on + strand at 323.874 kb on + strand at 323.889 kb on + strand at 323.892 kb on - strand at 323.892 kb on - strand at 323.892 kb on - strand at 323.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 320,576 - +2.6 320,654 - -0.2 320,723 + -2.6 320,728 + +0.1 320,729 + -0.6 320,729 - +0.6 320,731 - +2.6 320,781 - +0.8 320,788 - -0.1 320,788 - +0.4 320,807 + -0.3 320,813 + +1.2 320,815 - -1.1 320,821 - +0.1 320,865 - -0.2 320,883 - +0.0 320,884 - +0.1 320,886 + +0.4 320,886 + -0.9 320,894 + +0.9 320,894 + -0.8 320,898 + -0.2 320,898 + +0.1 320,899 - -0.0 320,901 - -0.2 320,904 - ykgE b0306 0.10 +0.8 320,944 + ykgE b0306 0.16 +0.3 320,944 + ykgE b0306 0.16 -0.5 320,959 + ykgE b0306 0.18 +1.3 320,959 + ykgE b0306 0.18 -0.1 320,971 - ykgE b0306 0.19 -0.1 320,975 - ykgE b0306 0.20 +0.1 320,975 - ykgE b0306 0.20 -0.1 320,977 - ykgE b0306 0.20 -0.6 320,978 - ykgE b0306 0.20 +0.3 320,983 + ykgE b0306 0.21 -0.2 321,022 + ykgE b0306 0.26 -0.5 321,027 + ykgE b0306 0.27 -0.4 321,027 + ykgE b0306 0.27 +0.4 321,035 - ykgE b0306 0.28 +0.2 321,065 + ykgE b0306 0.32 -0.1 321,073 - ykgE b0306 0.33 +0.4 321,106 - ykgE b0306 0.38 -0.3 321,202 - ykgE b0306 0.51 -0.4 321,221 - ykgE b0306 0.54 +0.2 321,221 - ykgE b0306 0.54 +0.6 321,236 - ykgE b0306 0.56 +0.5 321,236 - ykgE b0306 0.56 -0.5 321,249 - ykgE b0306 0.58 -0.0 321,266 + ykgE b0306 0.60 -0.5 321,307 + ykgE b0306 0.66 +0.2 321,321 + ykgE b0306 0.68 +0.7 321,325 + ykgE b0306 0.68 -0.7 321,325 + ykgE b0306 0.68 +0.2 321,335 + ykgE b0306 0.70 +0.0 321,436 - ykgE b0306 0.84 +0.6 321,461 + ykgE b0306 0.87 +0.2 321,461 + ykgE b0306 0.87 +0.5 321,533 + +0.4 321,533 + -0.0 321,552 + -0.0 321,553 + +0.3 321,557 + +0.5 321,672 - -0.0 321,699 + +0.6 321,732 + ykgF b0307 0.12 -0.8 321,732 + ykgF b0307 0.12 -1.1 321,736 + ykgF b0307 0.12 -0.2 321,750 + ykgF b0307 0.13 -0.0 321,918 + ykgF b0307 0.25 +0.5 321,960 + ykgF b0307 0.28 -0.4 321,960 + ykgF b0307 0.28 -0.3 322,013 + ykgF b0307 0.32 +0.1 322,028 + ykgF b0307 0.33 +0.4 322,028 + ykgF b0307 0.33 +1.1 322,034 + ykgF b0307 0.33 +0.1 322,075 + ykgF b0307 0.36 +1.2 322,081 + ykgF b0307 0.36 -0.0 322,154 + ykgF b0307 0.41 -0.2 322,239 + ykgF b0307 0.47 -0.1 322,477 - ykgF b0307 0.64 +1.3 322,516 + ykgF b0307 0.67 -0.2 322,516 + ykgF b0307 0.67 +0.1 322,560 - ykgF b0307 0.70 -1.3 322,641 + ykgF b0307 0.76 -0.2 322,660 + ykgF b0307 0.77 -0.2 323,074 + ykgG b0308 0.13 -0.2 323,074 + ykgG b0308 0.13 +0.0 323,094 + ykgG b0308 0.16 +0.5 323,105 + ykgG b0308 0.18 -0.1 323,168 + ykgG b0308 0.27 -0.2 323,237 + ykgG b0308 0.37 -0.3 323,307 - ykgG b0308 0.47 +2.6 323,346 - ykgG b0308 0.52 +1.6 323,346 - ykgG b0308 0.52 -0.2 323,359 + ykgG b0308 0.54 +0.4 323,359 + ykgG b0308 0.54 +0.3 323,500 + ykgG b0308 0.74 -0.1 323,503 + ykgG b0308 0.75 -0.4 323,503 - ykgG b0308 0.75 +0.6 323,583 - ykgG b0308 0.86 +0.2 323,612 - +0.8 323,614 + +0.0 323,614 + +0.2 323,621 - +0.8 323,623 + -0.8 323,623 + -0.0 323,623 + +0.4 323,628 + -0.1 323,628 + -0.6 323,630 + -0.1 323,630 + +0.3 323,630 + -0.0 323,630 + +0.5 323,630 + -0.0 323,630 + +0.3 323,630 + +0.8 323,651 + +0.0 323,690 + +0.1 323,696 + -0.6 323,698 - +2.6 323,701 + -0.3 323,701 + +0.0 323,712 + -0.3 323,712 + -0.4 323,712 + -0.1 323,712 + -0.1 323,742 + -0.5 323,748 + -0.5 323,748 + -0.0 323,748 + +0.5 323,749 + +0.7 323,757 + -0.1 323,757 + -0.2 323,759 + +1.0 323,759 + -1.0 323,759 + -0.1 323,874 + -1.0 323,874 + +1.4 323,889 + +1.0 323,892 - +2.6 323,892 - +1.0 323,892 - +2.6 323,892 - +0.3
Or see this region's nucleotide sequence