Experiment: Ying_Nucleobase14 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides
b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799
thrA
b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733
thrB
b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020
thrC
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1.868 kb on + strand, within thrA at 1.868 kb on + strand, within thrA at 1.871 kb on + strand, within thrA at 1.876 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.929 kb on + strand, within thrA at 1.929 kb on + strand, within thrA at 1.937 kb on - strand, within thrA at 1.937 kb on - strand, within thrA at 2.076 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.266 kb on - strand, within thrA at 2.266 kb on - strand, within thrA at 2.292 kb on - strand, within thrA at 2.294 kb on + strand, within thrA at 2.370 kb on - strand, within thrA at 2.370 kb on - strand, within thrA at 2.449 kb on - strand, within thrA at 2.590 kb on - strand at 2.675 kb on + strand at 2.682 kb on + strand at 2.682 kb on + strand at 2.684 kb on + strand at 2.717 kb on + strand at 2.744 kb on - strand at 2.787 kb on - strand at 2.787 kb on - strand at 2.829 kb on + strand at 2.847 kb on + strand at 2.847 kb on + strand at 2.906 kb on - strand, within thrB at 2.934 kb on + strand, within thrB at 2.963 kb on - strand, within thrB at 3.020 kb on - strand, within thrB at 3.023 kb on + strand, within thrB at 3.131 kb on + strand, within thrB at 3.144 kb on - strand, within thrB at 3.186 kb on + strand, within thrB at 3.246 kb on + strand, within thrB at 3.248 kb on + strand, within thrB at 3.256 kb on + strand, within thrB at 3.293 kb on + strand, within thrB at 3.301 kb on - strand, within thrB at 3.318 kb on - strand, within thrB at 3.411 kb on + strand, within thrB at 3.444 kb on - strand, within thrB at 3.444 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.509 kb on - strand, within thrB at 3.511 kb on + strand, within thrB at 3.607 kb on + strand, within thrB at 3.634 kb on - strand, within thrB at 3.638 kb on + strand, within thrB at 3.638 kb on + strand, within thrB at 3.650 kb on + strand at 3.769 kb on + strand at 3.769 kb on + strand at 3.792 kb on - strand at 3.805 kb on - strand at 3.953 kb on - strand, within thrC at 3.953 kb on - strand, within thrC at 4.108 kb on - strand, within thrC at 4.174 kb on - strand, within thrC at 4.180 kb on + strand, within thrC at 4.180 kb on + strand, within thrC at 4.216 kb on + strand, within thrC at 4.253 kb on - strand, within thrC at 4.253 kb on - strand, within thrC at 4.464 kb on - strand, within thrC at 4.466 kb on + strand, within thrC at 4.619 kb on + strand, within thrC at 4.721 kb on + strand, within thrC at 4.729 kb on + strand, within thrC at 4.729 kb on - strand, within thrC at 4.729 kb on - strand, within thrC
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep B; time point 2 remove 1,868 + thrA b0002 0.62 -4.5 1,868 + thrA b0002 0.62 -3.9 1,871 + thrA b0002 0.62 -4.7 1,876 - thrA b0002 0.62 -3.7 1,881 - thrA b0002 0.63 -5.0 1,881 - thrA b0002 0.63 -2.0 1,929 + thrA b0002 0.65 -5.7 1,929 + thrA b0002 0.65 -5.0 1,937 - thrA b0002 0.65 -2.1 1,937 - thrA b0002 0.65 -1.3 2,076 + thrA b0002 0.71 -4.7 2,104 + thrA b0002 0.72 -2.7 2,104 + thrA b0002 0.72 -4.4 2,266 - thrA b0002 0.78 -3.6 2,266 - thrA b0002 0.78 -4.1 2,292 - thrA b0002 0.79 -4.9 2,294 + thrA b0002 0.79 -4.6 2,370 - thrA b0002 0.83 -2.6 2,370 - thrA b0002 0.83 -3.0 2,449 - thrA b0002 0.86 -4.1 2,590 - -3.5 2,675 + -3.4 2,682 + -3.1 2,682 + -0.3 2,684 + -3.7 2,717 + -4.2 2,744 - -4.8 2,787 - -2.5 2,787 - -3.4 2,829 + -5.5 2,847 + -3.7 2,847 + -3.3 2,906 - thrB b0003 0.11 -3.5 2,934 + thrB b0003 0.14 -6.4 2,963 - thrB b0003 0.17 -4.0 3,020 - thrB b0003 0.23 +1.0 3,023 + thrB b0003 0.24 -4.5 3,131 + thrB b0003 0.35 -6.7 3,144 - thrB b0003 0.37 -4.2 3,186 + thrB b0003 0.41 -5.6 3,246 + thrB b0003 0.48 -3.8 3,248 + thrB b0003 0.48 -6.0 3,256 + thrB b0003 0.49 -5.8 3,293 + thrB b0003 0.53 -3.4 3,301 - thrB b0003 0.54 -3.4 3,318 - thrB b0003 0.55 -5.9 3,411 + thrB b0003 0.65 -4.7 3,444 - thrB b0003 0.69 -4.6 3,444 - thrB b0003 0.69 -2.5 3,504 - thrB b0003 0.75 -5.9 3,504 - thrB b0003 0.75 -3.8 3,509 - thrB b0003 0.76 -6.4 3,511 + thrB b0003 0.76 -4.5 3,607 + thrB b0003 0.86 -4.7 3,634 - thrB b0003 0.89 -3.7 3,638 + thrB b0003 0.90 -5.5 3,638 + thrB b0003 0.90 -3.3 3,650 + -3.7 3,769 + -3.6 3,769 + -4.0 3,792 - -3.2 3,805 - -3.9 3,953 - thrC b0004 0.17 -4.0 3,953 - thrC b0004 0.17 -4.9 4,108 - thrC b0004 0.29 -5.5 4,174 - thrC b0004 0.34 -4.7 4,180 + thrC b0004 0.35 -4.2 4,180 + thrC b0004 0.35 -4.1 4,216 + thrC b0004 0.37 -5.4 4,253 - thrC b0004 0.40 -4.0 4,253 - thrC b0004 0.40 -3.5 4,464 - thrC b0004 0.57 -3.7 4,466 + thrC b0004 0.57 -4.6 4,619 + thrC b0004 0.69 -4.6 4,721 + thrC b0004 0.77 -4.6 4,729 + thrC b0004 0.77 -4.4 4,729 - thrC b0004 0.77 -5.8 4,729 - thrC b0004 0.77 -4.1
Or see this region's nucleotide sequence