Strain Fitness in Escherichia coli BW25113 around b4520

Experiment: Ying_Nucleobase14 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb1168 and ymgF are separated by 131 nucleotidesymgF and ycgH are separated by 399 nucleotides b1168: b1168 - putative proteases (VIMSS), at 1,216,551 to 1,218,074 b1168 b4520: ymgF - hypothetical protein (NCBI), at 1,218,206 to 1,218,424 ymgF b4491: ycgH - no description, at 1,218,824 to 1,221,471 ycgH Position (kb) 1218 1219Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1217.360 kb on - strand, within b1168at 1217.640 kb on - strand, within b1168at 1217.667 kb on - strand, within b1168at 1217.690 kb on - strand, within b1168at 1217.692 kb on - strand, within b1168at 1217.764 kb on + strand, within b1168at 1217.764 kb on + strand, within b1168at 1217.767 kb on - strand, within b1168at 1217.856 kb on - strand, within b1168at 1217.861 kb on - strand, within b1168at 1217.891 kb on - strand, within b1168at 1217.891 kb on - strand, within b1168at 1217.891 kb on - strand, within b1168at 1218.016 kb on + strandat 1218.089 kb on + strandat 1218.096 kb on + strandat 1218.242 kb on - strand, within ymgFat 1218.284 kb on - strand, within ymgFat 1218.286 kb on - strand, within ymgFat 1218.301 kb on + strand, within ymgFat 1218.309 kb on - strand, within ymgFat 1218.311 kb on + strand, within ymgFat 1218.405 kb on + strandat 1218.414 kb on - strandat 1218.446 kb on + strandat 1218.450 kb on - strandat 1218.450 kb on - strandat 1218.454 kb on - strandat 1218.454 kb on - strandat 1218.468 kb on - strandat 1218.472 kb on - strandat 1218.478 kb on + strandat 1218.523 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2
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1,217,360 - b1168 b1168 0.53 +1.0
1,217,640 - b1168 b1168 0.71 -0.8
1,217,667 - b1168 b1168 0.73 +1.2
1,217,690 - b1168 b1168 0.75 +1.6
1,217,692 - b1168 b1168 0.75 -1.2
1,217,764 + b1168 b1168 0.80 +2.0
1,217,764 + b1168 b1168 0.80 -0.3
1,217,767 - b1168 b1168 0.80 +2.0
1,217,856 - b1168 b1168 0.86 +2.0
1,217,861 - b1168 b1168 0.86 -0.4
1,217,891 - b1168 b1168 0.88 +1.0
1,217,891 - b1168 b1168 0.88 +2.0
1,217,891 - b1168 b1168 0.88 +2.0
1,218,016 + +2.0
1,218,089 + -1.4
1,218,096 + +0.5
1,218,242 - ymgF b4520 0.16 +0.6
1,218,284 - ymgF b4520 0.36 +1.0
1,218,286 - ymgF b4520 0.37 +0.6
1,218,301 + ymgF b4520 0.43 +2.0
1,218,309 - ymgF b4520 0.47 +1.0
1,218,311 + ymgF b4520 0.48 +1.3
1,218,405 + -0.2
1,218,414 - -1.3
1,218,446 + +2.0
1,218,450 - -0.7
1,218,450 - -1.9
1,218,454 - +0.3
1,218,454 - -0.0
1,218,468 - +4.3
1,218,472 - +4.0
1,218,478 + +1.0
1,218,523 + +3.0

Or see this region's nucleotide sequence