Strain Fitness in Escherichia coli BW25113 around b2572

Experiment: Ying_Nucleobase14 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrseC and rseB overlap by 4 nucleotidesrseB and rseA overlap by 1 nucleotidesrseA and rpoE are separated by 32 nucleotides b2570: rseC - RseC protein involved in reduction of the SoxR iron-sulfur cluster (NCBI), at 2,705,344 to 2,705,823 rseC b2571: rseB - periplasmic negative regulator of sigmaE (NCBI), at 2,705,820 to 2,706,776 rseB b2572: rseA - anti-sigma factor (NCBI), at 2,706,776 to 2,707,426 rseA b2573: rpoE - RNA polymerase, sigma 24 (sigma E) factor (NCBI), at 2,707,459 to 2,708,034 rpoE Position (kb) 2706 2707 2708Strain fitness (log2 ratio) -2 -1 0 1 2at 2705.783 kb on - strandat 2705.783 kb on - strandat 2705.814 kb on - strandat 2705.915 kb on + strandat 2705.915 kb on + strandat 2705.923 kb on - strand, within rseBat 2705.987 kb on - strand, within rseBat 2706.013 kb on + strand, within rseBat 2706.190 kb on - strand, within rseBat 2706.190 kb on - strand, within rseBat 2706.211 kb on - strand, within rseBat 2706.307 kb on + strand, within rseBat 2706.315 kb on - strand, within rseBat 2706.315 kb on - strand, within rseBat 2706.322 kb on + strand, within rseBat 2706.397 kb on - strand, within rseBat 2706.399 kb on + strand, within rseBat 2706.524 kb on + strand, within rseBat 2706.524 kb on + strand, within rseBat 2706.576 kb on + strand, within rseBat 2706.601 kb on + strand, within rseBat 2706.601 kb on + strand, within rseBat 2706.654 kb on + strand, within rseBat 2706.696 kb on + strandat 2706.711 kb on - strandat 2706.711 kb on - strandat 2706.724 kb on - strandat 2706.738 kb on + strandat 2706.754 kb on - strandat 2706.754 kb on - strandat 2706.822 kb on - strandat 2706.822 kb on - strandat 2706.857 kb on - strand, within rseAat 2706.857 kb on - strand, within rseAat 2706.857 kb on - strand, within rseAat 2706.867 kb on - strand, within rseAat 2706.867 kb on - strand, within rseAat 2706.909 kb on - strand, within rseAat 2706.909 kb on - strand, within rseAat 2706.945 kb on - strand, within rseAat 2707.085 kb on - strand, within rseAat 2707.102 kb on - strand, within rseAat 2707.183 kb on + strand, within rseAat 2707.254 kb on + strand, within rseAat 2707.254 kb on + strand, within rseAat 2707.426 kb on - strandat 2707.436 kb on - strandat 2707.436 kb on - strandat 2707.437 kb on - strandat 2707.440 kb on + strandat 2708.179 kb on - strandat 2708.179 kb on - strandat 2708.218 kb on - strandat 2708.218 kb on - strandat 2708.221 kb on + strandat 2708.239 kb on - strandat 2708.289 kb on + strandat 2708.289 kb on + strandat 2708.309 kb on - strandat 2708.401 kb on - strandat 2708.406 kb on - strandat 2708.406 kb on - strandat 2708.411 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2
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2,705,783 - +0.3
2,705,783 - -0.4
2,705,814 - -0.2
2,705,915 + -0.4
2,705,915 + -0.5
2,705,923 - rseB b2571 0.11 +0.2
2,705,987 - rseB b2571 0.17 -0.2
2,706,013 + rseB b2571 0.20 -0.3
2,706,190 - rseB b2571 0.39 +0.4
2,706,190 - rseB b2571 0.39 +0.3
2,706,211 - rseB b2571 0.41 -0.6
2,706,307 + rseB b2571 0.51 -0.1
2,706,315 - rseB b2571 0.52 +0.5
2,706,315 - rseB b2571 0.52 +0.5
2,706,322 + rseB b2571 0.52 +0.4
2,706,397 - rseB b2571 0.60 -0.0
2,706,399 + rseB b2571 0.61 -0.1
2,706,524 + rseB b2571 0.74 +0.7
2,706,524 + rseB b2571 0.74 +0.0
2,706,576 + rseB b2571 0.79 +0.1
2,706,601 + rseB b2571 0.82 +0.1
2,706,601 + rseB b2571 0.82 -1.1
2,706,654 + rseB b2571 0.87 +0.1
2,706,696 + +0.1
2,706,711 - -0.0
2,706,711 - -0.1
2,706,724 - -0.3
2,706,738 + +0.0
2,706,754 - +0.0
2,706,754 - -0.5
2,706,822 - -0.3
2,706,822 - +0.5
2,706,857 - rseA b2572 0.12 -0.3
2,706,857 - rseA b2572 0.12 -0.1
2,706,857 - rseA b2572 0.12 -0.1
2,706,867 - rseA b2572 0.14 +0.6
2,706,867 - rseA b2572 0.14 +1.7
2,706,909 - rseA b2572 0.20 +0.9
2,706,909 - rseA b2572 0.20 +0.6
2,706,945 - rseA b2572 0.26 -1.3
2,707,085 - rseA b2572 0.47 +1.0
2,707,102 - rseA b2572 0.50 +2.0
2,707,183 + rseA b2572 0.63 -2.3
2,707,254 + rseA b2572 0.73 +2.7
2,707,254 + rseA b2572 0.73 +1.2
2,707,426 - +0.2
2,707,436 - -0.1
2,707,436 - -0.3
2,707,437 - +0.1
2,707,440 + -2.0
2,708,179 - +0.4
2,708,179 - -0.0
2,708,218 - -0.1
2,708,218 - +0.3
2,708,221 + +0.6
2,708,239 - -0.1
2,708,289 + +0.3
2,708,289 + -0.1
2,708,309 - -0.1
2,708,401 - -0.0
2,708,406 - -0.4
2,708,406 - -0.4
2,708,411 - -0.4

Or see this region's nucleotide sequence