Strain Fitness in Escherichia coli BW25113 around b1513

Experiment: Ying_Nucleobase14 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlsrR and lsrA are separated by 248 nucleotideslsrA and lsrC overlap by 7 nucleotides b1512: lsrR - lsr operon transcriptional repressor (NCBI), at 1,598,312 to 1,599,265 lsrR b1513: lsrA - fused AI2 transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 1,599,514 to 1,601,049 lsrA b1514: lsrC - AI2 transporter (NCBI), at 1,601,043 to 1,602,071 lsrC Position (kb) 1599 1600 1601 1602Strain fitness (log2 ratio) -1 0 1at 1598.582 kb on + strand, within lsrRat 1598.950 kb on + strand, within lsrRat 1598.950 kb on + strand, within lsrRat 1598.997 kb on - strand, within lsrRat 1599.098 kb on - strand, within lsrRat 1599.110 kb on - strand, within lsrRat 1599.478 kb on + strandat 1599.478 kb on + strandat 1599.628 kb on + strandat 1599.697 kb on - strand, within lsrAat 1599.697 kb on - strand, within lsrAat 1599.928 kb on - strand, within lsrAat 1599.928 kb on - strand, within lsrAat 1600.439 kb on + strand, within lsrAat 1600.518 kb on + strand, within lsrAat 1600.666 kb on - strand, within lsrAat 1600.952 kb on + strandat 1601.276 kb on - strand, within lsrCat 1601.423 kb on + strand, within lsrCat 1601.423 kb on + strand, within lsrCat 1601.973 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2
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1,598,582 + lsrR b1512 0.28 -0.1
1,598,950 + lsrR b1512 0.67 +0.8
1,598,950 + lsrR b1512 0.67 +0.7
1,598,997 - lsrR b1512 0.72 -0.5
1,599,098 - lsrR b1512 0.82 +0.8
1,599,110 - lsrR b1512 0.84 -0.6
1,599,478 + -0.1
1,599,478 + +0.0
1,599,628 + +0.1
1,599,697 - lsrA b1513 0.12 +0.2
1,599,697 - lsrA b1513 0.12 +0.6
1,599,928 - lsrA b1513 0.27 +1.0
1,599,928 - lsrA b1513 0.27 +0.5
1,600,439 + lsrA b1513 0.60 +0.4
1,600,518 + lsrA b1513 0.65 +0.6
1,600,666 - lsrA b1513 0.75 +0.3
1,600,952 + +0.4
1,601,276 - lsrC b1514 0.23 +0.6
1,601,423 + lsrC b1514 0.37 +0.4
1,601,423 + lsrC b1514 0.37 +0.6
1,601,973 + -0.6

Or see this region's nucleotide sequence