Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymfB and ymfC are separated by 9 nucleotides ymfC and icd are separated by 171 nucleotides icd and ymfD are separated by 493 nucleotides
b1134: ymfB - bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI), at 1,193,050 to 1,193,511
ymfB
b1135: ymfC - orf, hypothetical protein (VIMSS), at 1,193,521 to 1,194,174
ymfC
b1136: icd - isocitrate dehydrogenase (NCBI), at 1,194,346 to 1,195,596
icd
b1137: ymfD - e14 prophage; predicted SAM-dependent methyltransferase (NCBI), at 1,196,090 to 1,196,755
ymfD
Position (kb)
1194
1195
1196 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1193.439 kb on - strand, within ymfB at 1193.469 kb on + strand at 1193.558 kb on - strand at 1193.669 kb on - strand, within ymfC at 1193.697 kb on - strand, within ymfC at 1193.774 kb on - strand, within ymfC at 1193.778 kb on + strand, within ymfC at 1193.780 kb on - strand, within ymfC at 1193.825 kb on + strand, within ymfC at 1193.886 kb on - strand, within ymfC at 1195.722 kb on - strand at 1195.722 kb on - strand at 1195.729 kb on + strand at 1195.735 kb on + strand at 1195.740 kb on + strand at 1195.740 kb on + strand at 1195.740 kb on + strand at 1195.741 kb on + strand at 1195.746 kb on - strand at 1195.749 kb on + strand at 1195.760 kb on + strand at 1195.807 kb on + strand at 1195.888 kb on + strand at 1195.894 kb on - strand at 1195.894 kb on - strand at 1195.894 kb on - strand at 1195.895 kb on - strand at 1195.895 kb on - strand at 1195.896 kb on - strand at 1195.896 kb on - strand at 1195.896 kb on - strand at 1195.932 kb on + strand at 1195.932 kb on + strand at 1195.938 kb on - strand at 1195.941 kb on - strand at 1195.944 kb on + strand at 1195.944 kb on + strand at 1195.954 kb on + strand at 1195.957 kb on - strand at 1195.961 kb on - strand at 1195.963 kb on - strand at 1195.972 kb on - strand at 1195.982 kb on - strand at 1195.985 kb on + strand at 1196.004 kb on - strand at 1196.045 kb on + strand at 1196.045 kb on + strand at 1196.054 kb on + strand at 1196.105 kb on - strand at 1196.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 1,193,439 - ymfB b1134 0.84 -0.1 1,193,469 + -0.2 1,193,558 - -0.5 1,193,669 - ymfC b1135 0.23 +0.0 1,193,697 - ymfC b1135 0.27 +0.2 1,193,774 - ymfC b1135 0.39 -0.7 1,193,778 + ymfC b1135 0.39 +0.0 1,193,780 - ymfC b1135 0.40 -0.1 1,193,825 + ymfC b1135 0.46 +0.5 1,193,886 - ymfC b1135 0.56 -0.3 1,195,722 - -0.7 1,195,722 - -0.2 1,195,729 + -0.2 1,195,735 + +0.0 1,195,740 + -0.9 1,195,740 + +0.1 1,195,740 + +0.2 1,195,741 + +0.1 1,195,746 - +0.3 1,195,749 + -0.2 1,195,760 + -0.5 1,195,807 + -0.3 1,195,888 + +0.1 1,195,894 - +0.5 1,195,894 - -0.1 1,195,894 - +0.4 1,195,895 - -0.0 1,195,895 - -0.2 1,195,896 - +0.7 1,195,896 - +3.0 1,195,896 - +1.8 1,195,932 + -0.4 1,195,932 + +0.2 1,195,938 - -0.2 1,195,941 - -0.0 1,195,944 + +0.3 1,195,944 + +0.3 1,195,954 + +0.5 1,195,957 - -0.5 1,195,961 - +0.9 1,195,963 - +0.6 1,195,972 - -0.3 1,195,982 - -0.0 1,195,985 + +0.1 1,196,004 - +0.3 1,196,045 + +2.0 1,196,045 + +0.6 1,196,054 + +1.0 1,196,105 - -1.9 1,196,127 - +1.4
Or see this region's nucleotide sequence