Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ssuB and ycbM overlap by 4 nucleotides ycbM and ssuD are separated by 10 nucleotides ssuD and ycbO overlap by 4 nucleotides ycbO and ssuE overlap by 8 nucleotides
b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267
ssuB
b0934: ycbM - putative transport system permease protein (VIMSS), at 993,264 to 994,055
ycbM
b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211
ssuD
b0936: ycbO - orf, hypothetical protein (VIMSS), at 995,208 to 996,167
ycbO
b0937: ssuE - NAD(P)H-dependent FMN reductase (NCBI), at 996,160 to 996,735
ssuE
Position (kb) 994
995
996 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 993.078 kb on - strand, within ssuB at 993.103 kb on + strand, within ssuB at 993.239 kb on - strand at 993.526 kb on + strand, within ycbM at 993.729 kb on - strand, within ycbM at 993.913 kb on + strand, within ycbM at 994.024 kb on + strand at 994.033 kb on - strand at 994.089 kb on + strand at 994.089 kb on + strand at 994.335 kb on - strand, within ssuD at 994.356 kb on + strand, within ssuD at 994.364 kb on - strand, within ssuD at 994.597 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.723 kb on + strand, within ssuD at 994.869 kb on + strand, within ssuD at 994.869 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.962 kb on + strand, within ssuD at 995.043 kb on - strand, within ssuD at 995.236 kb on - strand at 995.236 kb on - strand at 995.473 kb on - strand, within ycbO at 995.512 kb on + strand, within ycbO at 995.607 kb on + strand, within ycbO at 995.616 kb on + strand, within ycbO at 995.631 kb on + strand, within ycbO at 995.734 kb on - strand, within ycbO at 995.753 kb on - strand, within ycbO at 995.832 kb on - strand, within ycbO at 995.871 kb on + strand, within ycbO at 995.871 kb on + strand, within ycbO at 996.185 kb on + strand at 996.207 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 993,078 - ssuB b0933 0.75 -1.3 993,103 + ssuB b0933 0.79 +0.7 993,239 - -0.7 993,526 + ycbM b0934 0.33 +0.4 993,729 - ycbM b0934 0.59 +0.2 993,913 + ycbM b0934 0.82 -0.4 994,024 + -0.8 994,033 - -0.1 994,089 + -0.5 994,089 + +0.5 994,335 - ssuD b0935 0.23 +4.4 994,356 + ssuD b0935 0.25 +0.3 994,364 - ssuD b0935 0.26 +0.1 994,597 - ssuD b0935 0.46 +0.2 994,710 - ssuD b0935 0.56 -0.1 994,710 - ssuD b0935 0.56 +0.2 994,723 + ssuD b0935 0.57 +0.4 994,869 + ssuD b0935 0.70 +0.0 994,869 - ssuD b0935 0.70 +0.3 994,900 - ssuD b0935 0.73 -0.1 994,900 - ssuD b0935 0.73 -0.1 994,962 + ssuD b0935 0.78 +0.2 995,043 - ssuD b0935 0.85 +0.1 995,236 - -2.0 995,236 - +0.0 995,473 - ycbO b0936 0.28 -0.4 995,512 + ycbO b0936 0.32 -0.3 995,607 + ycbO b0936 0.42 +0.2 995,616 + ycbO b0936 0.42 +0.1 995,631 + ycbO b0936 0.44 -0.2 995,734 - ycbO b0936 0.55 +0.5 995,753 - ycbO b0936 0.57 +0.0 995,832 - ycbO b0936 0.65 -0.8 995,871 + ycbO b0936 0.69 -0.1 995,871 + ycbO b0936 0.69 -0.9 996,185 + +0.2 996,207 - -0.0
Or see this region's nucleotide sequence