Experiment: Ying_Nucleobase14 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galK and galT are separated by 3 nucleotides galT and galE are separated by 9 nucleotides
b0757: galK - galactokinase GalK (EC 2.7.1.6) <small>(from data)</small>, at 788,054 to 789,202
galK
b0758: galT - galactose-1-phosphate uridylyltransferase (NCBI), at 789,206 to 790,252
galT
b0759: galE - UDP-galactose-4-epimerase (VIMSS), at 790,262 to 791,278
galE
Position (kb) 789
790
791 Strain fitness (log2 ratio) -1
0
1 at 788.252 kb on + strand, within galK at 788.406 kb on - strand, within galK at 788.406 kb on - strand, within galK at 788.436 kb on - strand, within galK at 788.479 kb on + strand, within galK at 788.479 kb on + strand, within galK at 788.487 kb on - strand, within galK at 788.487 kb on - strand, within galK at 788.518 kb on - strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.531 kb on + strand, within galK at 788.600 kb on - strand, within galK at 788.610 kb on + strand, within galK at 788.644 kb on - strand, within galK at 788.648 kb on - strand, within galK at 788.732 kb on + strand, within galK at 788.829 kb on + strand, within galK at 788.832 kb on - strand, within galK at 789.031 kb on + strand, within galK at 789.039 kb on - strand, within galK at 789.050 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.063 kb on + strand, within galK at 789.241 kb on - strand at 789.338 kb on - strand, within galT at 789.338 kb on - strand, within galT at 789.425 kb on - strand, within galT at 789.425 kb on - strand at 789.433 kb on - strand, within galT at 789.581 kb on - strand, within galT at 789.661 kb on + strand, within galT at 789.712 kb on - strand, within galT at 789.712 kb on - strand, within galT at 789.774 kb on - strand, within galT at 789.811 kb on + strand, within galT at 789.908 kb on + strand, within galT at 790.161 kb on - strand at 790.244 kb on - strand at 790.244 kb on - strand at 790.244 kb on - strand at 790.264 kb on + strand at 790.264 kb on + strand at 790.298 kb on + strand at 790.385 kb on + strand, within galE at 790.385 kb on + strand, within galE at 790.393 kb on + strand, within galE at 790.499 kb on + strand, within galE at 790.499 kb on + strand, within galE at 790.508 kb on - strand, within galE at 790.541 kb on + strand, within galE at 790.604 kb on - strand, within galE at 790.604 kb on - strand, within galE at 790.658 kb on + strand, within galE at 790.658 kb on + strand, within galE at 790.668 kb on + strand, within galE at 790.719 kb on + strand, within galE at 790.759 kb on + strand, within galE at 790.950 kb on + strand, within galE at 790.950 kb on + strand, within galE at 791.027 kb on + strand, within galE at 791.027 kb on + strand, within galE at 791.152 kb on - strand, within galE at 791.174 kb on - strand, within galE
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Nucleobase14 rep A; time point 2 remove 788,252 + galK b0757 0.17 -0.3 788,406 - galK b0757 0.31 -1.5 788,406 - galK b0757 0.31 -0.9 788,436 - galK b0757 0.33 +0.0 788,479 + galK b0757 0.37 -0.9 788,479 + galK b0757 0.37 -1.2 788,487 - galK b0757 0.38 +0.3 788,487 - galK b0757 0.38 -0.4 788,518 - galK b0757 0.40 -0.7 788,531 + galK b0757 0.42 -0.4 788,531 + galK b0757 0.42 -0.6 788,531 + galK b0757 0.42 +1.0 788,531 + galK b0757 0.42 +0.3 788,600 - galK b0757 0.48 -0.5 788,610 + galK b0757 0.48 -0.3 788,644 - galK b0757 0.51 -0.1 788,648 - galK b0757 0.52 -0.1 788,732 + galK b0757 0.59 -0.5 788,829 + galK b0757 0.67 -0.4 788,832 - galK b0757 0.68 -0.3 789,031 + galK b0757 0.85 -0.7 789,039 - galK b0757 0.86 -0.4 789,050 + galK b0757 0.87 -0.5 789,063 + galK b0757 0.88 -0.2 789,063 + galK b0757 0.88 +0.7 789,241 - +0.3 789,338 - galT b0758 0.13 -0.4 789,338 - galT b0758 0.13 -0.1 789,425 - galT b0758 0.21 +0.0 789,425 - -0.6 789,433 - galT b0758 0.22 +0.4 789,581 - galT b0758 0.36 -0.1 789,661 + galT b0758 0.43 -1.0 789,712 - galT b0758 0.48 -0.1 789,712 - galT b0758 0.48 -0.8 789,774 - galT b0758 0.54 -0.7 789,811 + galT b0758 0.58 -1.2 789,908 + galT b0758 0.67 +0.2 790,161 - -0.9 790,244 - +0.2 790,244 - -0.1 790,244 - -0.6 790,264 + -0.4 790,264 + +0.7 790,298 + +0.2 790,385 + galE b0759 0.12 +1.0 790,385 + galE b0759 0.12 +0.5 790,393 + galE b0759 0.13 +0.6 790,499 + galE b0759 0.23 +0.4 790,499 + galE b0759 0.23 +0.6 790,508 - galE b0759 0.24 +0.5 790,541 + galE b0759 0.27 +0.0 790,604 - galE b0759 0.34 +0.2 790,604 - galE b0759 0.34 +0.6 790,658 + galE b0759 0.39 +0.5 790,658 + galE b0759 0.39 -0.1 790,668 + galE b0759 0.40 +0.3 790,719 + galE b0759 0.45 -0.8 790,759 + galE b0759 0.49 +0.5 790,950 + galE b0759 0.68 +0.5 790,950 + galE b0759 0.68 +0.3 791,027 + galE b0759 0.75 -0.4 791,027 + galE b0759 0.75 +0.7 791,152 - galE b0759 0.88 +0.0 791,174 - galE b0759 0.90 +0.1
Or see this region's nucleotide sequence