Experiment: Ying_sugar7 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagA and nagB are separated by 59 nucleotides nagB and nagE are separated by 332 nucleotides
b0677: nagA - N-acetylglucosamine-6-phosphate deacetylase (NCBI), at 700,826 to 701,974
nagA
b0678: nagB - glucosamine-6-phosphate deaminase (NCBI), at 702,034 to 702,834
nagB
b0679: nagE - fused N-acetyl glucosamine specific PTS enzyme: IIC, IIB , and IIA components (NCBI), at 703,167 to 705,113
nagE
Position (kb) 702
703 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 701.074 kb on - strand, within nagA at 701.191 kb on + strand, within nagA at 701.244 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.309 kb on - strand, within nagA at 701.321 kb on + strand, within nagA at 701.327 kb on - strand, within nagA at 701.347 kb on - strand, within nagA at 701.382 kb on + strand, within nagA at 701.417 kb on + strand, within nagA at 701.498 kb on - strand, within nagA at 701.531 kb on - strand, within nagA at 701.565 kb on + strand, within nagA at 701.565 kb on + strand, within nagA at 701.648 kb on + strand, within nagA at 701.692 kb on + strand, within nagA at 701.694 kb on - strand, within nagA at 701.755 kb on + strand, within nagA at 701.766 kb on + strand, within nagA at 701.805 kb on - strand, within nagA at 701.829 kb on + strand, within nagA at 701.904 kb on - strand at 701.904 kb on - strand at 701.959 kb on + strand at 701.959 kb on + strand at 701.959 kb on + strand at 701.968 kb on + strand at 701.980 kb on - strand at 702.001 kb on + strand at 702.001 kb on + strand at 702.011 kb on - strand at 702.020 kb on - strand at 702.104 kb on + strand at 702.160 kb on - strand, within nagB at 702.160 kb on - strand, within nagB at 702.166 kb on + strand, within nagB at 702.180 kb on - strand, within nagB at 702.217 kb on - strand, within nagB at 702.250 kb on + strand, within nagB at 702.346 kb on + strand, within nagB at 702.619 kb on - strand, within nagB at 702.624 kb on + strand, within nagB at 702.624 kb on + strand, within nagB at 702.769 kb on + strand at 702.794 kb on + strand at 702.804 kb on + strand at 702.823 kb on + strand at 702.823 kb on + strand at 702.881 kb on + strand at 702.966 kb on + strand at 703.054 kb on + strand at 703.054 kb on + strand at 703.064 kb on + strand at 703.087 kb on + strand at 703.193 kb on + strand at 703.193 kb on + strand at 703.211 kb on + strand at 703.420 kb on + strand, within nagE at 703.464 kb on - strand, within nagE at 703.634 kb on + strand, within nagE at 703.639 kb on - strand, within nagE at 703.663 kb on + strand, within nagE at 703.692 kb on - strand, within nagE at 703.711 kb on - strand, within nagE at 703.711 kb on - strand, within nagE at 703.742 kb on + strand, within nagE at 703.786 kb on - strand, within nagE at 703.786 kb on - strand, within nagE at 703.797 kb on + strand, within nagE at 703.815 kb on + strand, within nagE at 703.817 kb on - strand, within nagE
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep B; time point 2 remove 701,074 - nagA b0677 0.22 -1.5 701,191 + nagA b0677 0.32 -0.9 701,244 - nagA b0677 0.36 -2.4 701,309 - nagA b0677 0.42 -1.2 701,309 - nagA b0677 0.42 -1.3 701,321 + nagA b0677 0.43 -0.0 701,327 - nagA b0677 0.44 -1.5 701,347 - nagA b0677 0.45 -2.2 701,382 + nagA b0677 0.48 -2.4 701,417 + nagA b0677 0.51 -1.3 701,498 - nagA b0677 0.58 -2.1 701,531 - nagA b0677 0.61 -2.1 701,565 + nagA b0677 0.64 -2.3 701,565 + nagA b0677 0.64 -2.5 701,648 + nagA b0677 0.72 -2.9 701,692 + nagA b0677 0.75 -1.3 701,694 - nagA b0677 0.76 -3.0 701,755 + nagA b0677 0.81 -2.2 701,766 + nagA b0677 0.82 -1.3 701,805 - nagA b0677 0.85 -2.3 701,829 + nagA b0677 0.87 -4.1 701,904 - -2.3 701,904 - -3.4 701,959 + -1.8 701,959 + -3.8 701,959 + -3.4 701,968 + -4.8 701,980 - -0.0 702,001 + +0.7 702,001 + -0.1 702,011 - +0.2 702,020 - +0.3 702,104 + -5.7 702,160 - nagB b0678 0.16 -3.8 702,160 - nagB b0678 0.16 -4.0 702,166 + nagB b0678 0.16 -5.5 702,180 - nagB b0678 0.18 -5.1 702,217 - nagB b0678 0.23 -5.4 702,250 + nagB b0678 0.27 -4.3 702,346 + nagB b0678 0.39 -5.4 702,619 - nagB b0678 0.73 -5.9 702,624 + nagB b0678 0.74 -3.3 702,624 + nagB b0678 0.74 -4.1 702,769 + -4.2 702,794 + -5.5 702,804 + -3.8 702,823 + -3.5 702,823 + -4.0 702,881 + -4.4 702,966 + -0.9 703,054 + -1.1 703,054 + -1.2 703,064 + -0.1 703,087 + +0.8 703,193 + +0.2 703,193 + +0.0 703,211 + +0.2 703,420 + nagE b0679 0.13 -0.4 703,464 - nagE b0679 0.15 -0.0 703,634 + nagE b0679 0.24 -0.1 703,639 - nagE b0679 0.24 +0.4 703,663 + nagE b0679 0.25 -0.1 703,692 - nagE b0679 0.27 +0.4 703,711 - nagE b0679 0.28 -0.6 703,711 - nagE b0679 0.28 -0.1 703,742 + nagE b0679 0.30 +0.6 703,786 - nagE b0679 0.32 +0.4 703,786 - nagE b0679 0.32 +1.1 703,797 + nagE b0679 0.32 -0.1 703,815 + nagE b0679 0.33 +0.1 703,817 - nagE b0679 0.33 +0.4
Or see this region's nucleotide sequence