Experiment: Ying_sugar7 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjjJ and lplA are separated by 0 nucleotides lplA and ytjB are separated by 27 nucleotides ytjB and serB are separated by 105 nucleotides
b4385: yjjJ - predicted DNA-binding transcriptional regulator (NCBI), at 4,619,792 to 4,621,123
yjjJ
b4386: lplA - lipoate-protein ligase A (NCBI), at 4,621,124 to 4,622,140
lplA
b4387: ytjB - hypothetical protein (NCBI), at 4,622,168 to 4,622,812
ytjB
b4388: serB - 3-phosphoserine phosphatase (NCBI), at 4,622,918 to 4,623,886
serB
Position (kb)
4621
4622
4623 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 4620.193 kb on - strand, within yjjJ at 4620.217 kb on + strand, within yjjJ at 4620.305 kb on - strand, within yjjJ at 4620.335 kb on - strand, within yjjJ at 4620.350 kb on - strand, within yjjJ at 4620.350 kb on - strand, within yjjJ at 4620.455 kb on - strand, within yjjJ at 4620.484 kb on - strand, within yjjJ at 4620.581 kb on - strand, within yjjJ at 4620.583 kb on + strand, within yjjJ at 4620.608 kb on - strand, within yjjJ at 4620.627 kb on - strand, within yjjJ at 4620.632 kb on - strand, within yjjJ at 4620.677 kb on + strand, within yjjJ at 4620.688 kb on + strand, within yjjJ at 4620.835 kb on - strand, within yjjJ at 4620.883 kb on - strand, within yjjJ at 4620.954 kb on + strand, within yjjJ at 4620.993 kb on + strand at 4621.019 kb on - strand at 4621.117 kb on - strand at 4621.117 kb on - strand at 4621.124 kb on + strand at 4621.128 kb on + strand at 4621.128 kb on + strand at 4621.232 kb on + strand, within lplA at 4621.240 kb on - strand, within lplA at 4621.240 kb on - strand, within lplA at 4621.240 kb on - strand, within lplA at 4621.287 kb on - strand, within lplA at 4621.298 kb on + strand, within lplA at 4621.298 kb on + strand, within lplA at 4621.301 kb on - strand, within lplA at 4621.350 kb on - strand, within lplA at 4621.350 kb on - strand, within lplA at 4621.457 kb on + strand, within lplA at 4621.457 kb on + strand, within lplA at 4621.491 kb on + strand, within lplA at 4621.501 kb on + strand, within lplA at 4621.549 kb on + strand, within lplA at 4621.549 kb on + strand, within lplA at 4621.592 kb on + strand, within lplA at 4621.674 kb on - strand, within lplA at 4621.674 kb on - strand, within lplA at 4621.869 kb on + strand, within lplA at 4621.884 kb on - strand, within lplA at 4621.946 kb on - strand, within lplA at 4621.946 kb on - strand, within lplA at 4621.952 kb on - strand, within lplA at 4621.957 kb on - strand, within lplA at 4621.970 kb on + strand, within lplA at 4621.970 kb on + strand, within lplA at 4621.976 kb on - strand, within lplA at 4622.011 kb on - strand, within lplA at 4622.011 kb on - strand, within lplA at 4622.126 kb on + strand at 4622.281 kb on - strand, within ytjB at 4622.281 kb on - strand, within ytjB at 4622.284 kb on - strand, within ytjB at 4622.284 kb on - strand, within ytjB at 4622.289 kb on - strand, within ytjB at 4622.305 kb on + strand, within ytjB at 4622.531 kb on + strand, within ytjB at 4622.534 kb on + strand, within ytjB at 4622.539 kb on - strand, within ytjB at 4622.539 kb on - strand, within ytjB at 4622.637 kb on - strand, within ytjB at 4622.653 kb on + strand, within ytjB at 4622.705 kb on - strand, within ytjB at 4622.816 kb on - strand at 4622.846 kb on + strand at 4622.901 kb on + strand at 4622.901 kb on + strand at 4622.925 kb on + strand at 4622.925 kb on + strand at 4622.925 kb on + strand at 4622.944 kb on + strand at 4622.952 kb on - strand at 4622.952 kb on - strand at 4622.977 kb on - strand at 4623.023 kb on + strand, within serB at 4623.023 kb on - strand, within serB at 4623.038 kb on + strand, within serB at 4623.038 kb on + strand, within serB at 4623.048 kb on + strand, within serB at 4623.048 kb on + strand, within serB at 4623.056 kb on - strand, within serB at 4623.109 kb on - strand, within serB at 4623.126 kb on - strand, within serB at 4623.126 kb on - strand, within serB
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 2 remove 4,620,193 - yjjJ b4385 0.30 +0.2 4,620,217 + yjjJ b4385 0.32 +0.2 4,620,305 - yjjJ b4385 0.39 -0.4 4,620,335 - yjjJ b4385 0.41 +0.7 4,620,350 - yjjJ b4385 0.42 +0.7 4,620,350 - yjjJ b4385 0.42 +0.1 4,620,455 - yjjJ b4385 0.50 +0.1 4,620,484 - yjjJ b4385 0.52 -0.0 4,620,581 - yjjJ b4385 0.59 +0.8 4,620,583 + yjjJ b4385 0.59 -0.3 4,620,608 - yjjJ b4385 0.61 -0.1 4,620,627 - yjjJ b4385 0.63 -0.4 4,620,632 - yjjJ b4385 0.63 +0.5 4,620,677 + yjjJ b4385 0.66 +0.1 4,620,688 + yjjJ b4385 0.67 +0.7 4,620,835 - yjjJ b4385 0.78 +0.7 4,620,883 - yjjJ b4385 0.82 -1.9 4,620,954 + yjjJ b4385 0.87 +0.5 4,620,993 + -0.5 4,621,019 - -0.1 4,621,117 - +0.1 4,621,117 - -0.5 4,621,124 + -0.0 4,621,128 + +0.0 4,621,128 + +0.6 4,621,232 + lplA b4386 0.11 +0.5 4,621,240 - lplA b4386 0.11 -1.5 4,621,240 - lplA b4386 0.11 -0.4 4,621,240 - lplA b4386 0.11 +0.3 4,621,287 - lplA b4386 0.16 -0.1 4,621,298 + lplA b4386 0.17 -2.3 4,621,298 + lplA b4386 0.17 -0.1 4,621,301 - lplA b4386 0.17 -0.4 4,621,350 - lplA b4386 0.22 -0.4 4,621,350 - lplA b4386 0.22 -0.6 4,621,457 + lplA b4386 0.33 -0.1 4,621,457 + lplA b4386 0.33 +0.1 4,621,491 + lplA b4386 0.36 +0.0 4,621,501 + lplA b4386 0.37 -0.4 4,621,549 + lplA b4386 0.42 -0.8 4,621,549 + lplA b4386 0.42 -0.3 4,621,592 + lplA b4386 0.46 +0.4 4,621,674 - lplA b4386 0.54 +0.1 4,621,674 - lplA b4386 0.54 +0.0 4,621,869 + lplA b4386 0.73 -0.5 4,621,884 - lplA b4386 0.75 +0.1 4,621,946 - lplA b4386 0.81 +0.6 4,621,946 - lplA b4386 0.81 +0.2 4,621,952 - lplA b4386 0.81 +0.5 4,621,957 - lplA b4386 0.82 -0.0 4,621,970 + lplA b4386 0.83 -0.3 4,621,970 + lplA b4386 0.83 -0.1 4,621,976 - lplA b4386 0.84 -0.5 4,622,011 - lplA b4386 0.87 +0.1 4,622,011 - lplA b4386 0.87 -0.5 4,622,126 + +0.8 4,622,281 - ytjB b4387 0.18 -0.4 4,622,281 - ytjB b4387 0.18 +0.3 4,622,284 - ytjB b4387 0.18 -0.2 4,622,284 - ytjB b4387 0.18 -1.9 4,622,289 - ytjB b4387 0.19 +0.1 4,622,305 + ytjB b4387 0.21 +0.2 4,622,531 + ytjB b4387 0.56 -0.7 4,622,534 + ytjB b4387 0.57 -0.1 4,622,539 - ytjB b4387 0.58 +0.1 4,622,539 - ytjB b4387 0.58 +0.0 4,622,637 - ytjB b4387 0.73 +0.3 4,622,653 + ytjB b4387 0.75 +0.0 4,622,705 - ytjB b4387 0.83 +0.0 4,622,816 - +0.0 4,622,846 + -0.8 4,622,901 + +0.1 4,622,901 + -0.6 4,622,925 + -1.4 4,622,925 + -4.2 4,622,925 + -4.7 4,622,944 + +0.4 4,622,952 - -4.0 4,622,952 - -4.4 4,622,977 - -6.2 4,623,023 + serB b4388 0.11 -3.8 4,623,023 - serB b4388 0.11 -4.3 4,623,038 + serB b4388 0.12 -3.9 4,623,038 + serB b4388 0.12 -3.9 4,623,048 + serB b4388 0.13 -3.7 4,623,048 + serB b4388 0.13 -3.8 4,623,056 - serB b4388 0.14 -5.7 4,623,109 - serB b4388 0.20 -4.1 4,623,126 - serB b4388 0.21 -3.9 4,623,126 - serB b4388 0.21 -4.4
Or see this region's nucleotide sequence