Experiment: Ying_sugar7 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagE and glnS are separated by 202 nucleotides glnS and ybfM are separated by 576 nucleotides
b0679: nagE - fused N-acetyl glucosamine specific PTS enzyme: IIC, IIB , and IIA components (NCBI), at 703,167 to 705,113
nagE
b0680: glnS - glutaminyl-tRNA synthetase (NCBI), at 705,316 to 706,980
glnS
b0681: ybfM - predicted outer membrane porin (NCBI), at 707,557 to 708,963
ybfM
Position (kb) 705
706
707 Strain fitness (log2 ratio) -1
0
1 at 704.356 kb on + strand, within nagE at 704.464 kb on - strand, within nagE at 704.500 kb on - strand, within nagE at 704.513 kb on - strand, within nagE at 704.513 kb on - strand, within nagE at 704.532 kb on + strand, within nagE at 704.648 kb on - strand, within nagE at 704.648 kb on - strand, within nagE at 704.648 kb on - strand, within nagE at 704.686 kb on - strand, within nagE at 704.726 kb on + strand, within nagE at 704.787 kb on - strand, within nagE at 704.800 kb on - strand, within nagE at 704.876 kb on + strand, within nagE at 704.876 kb on + strand, within nagE at 704.922 kb on + strand at 704.942 kb on - strand at 705.090 kb on - strand at 705.090 kb on - strand at 705.257 kb on - strand at 705.281 kb on + strand at 705.302 kb on + strand at 706.950 kb on + strand at 706.950 kb on + strand at 707.325 kb on - strand at 707.325 kb on - strand at 707.356 kb on - strand at 707.356 kb on - strand at 707.456 kb on + strand at 707.546 kb on - strand at 707.566 kb on - strand at 707.566 kb on - strand at 707.664 kb on - strand at 707.834 kb on + strand, within ybfM at 707.917 kb on + strand, within ybfM at 707.945 kb on + strand, within ybfM
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_sugar7 rep A; time point 2 remove 704,356 + nagE b0679 0.61 +0.4 704,464 - nagE b0679 0.67 -0.4 704,500 - nagE b0679 0.68 +0.4 704,513 - nagE b0679 0.69 +0.6 704,513 - nagE b0679 0.69 +0.8 704,532 + nagE b0679 0.70 -0.6 704,648 - nagE b0679 0.76 +0.1 704,648 - nagE b0679 0.76 +0.6 704,648 - nagE b0679 0.76 -0.3 704,686 - nagE b0679 0.78 -0.0 704,726 + nagE b0679 0.80 +0.6 704,787 - nagE b0679 0.83 -0.2 704,800 - nagE b0679 0.84 +0.3 704,876 + nagE b0679 0.88 +0.4 704,876 + nagE b0679 0.88 +0.2 704,922 + -0.3 704,942 - +0.4 705,090 - +0.4 705,090 - -0.0 705,257 - -0.6 705,281 + +0.3 705,302 + -0.2 706,950 + -0.6 706,950 + -1.0 707,325 - +0.5 707,325 - -0.2 707,356 - +0.4 707,356 - +0.0 707,456 + -1.3 707,546 - -1.0 707,566 - +0.5 707,566 - +0.1 707,664 - +1.0 707,834 + ybfM b0681 0.20 +0.2 707,917 + ybfM b0681 0.26 +0.1 707,945 + ybfM b0681 0.28 -1.3
Or see this region's nucleotide sequence