Strain Fitness in Escherichia coli BW25113 around b3206

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntptsN and yhbJ are separated by 45 nucleotidesyhbJ and npr overlap by 4 nucleotidesnpr and yrbL are separated by 213 nucleotidesyrbL and mtgA overlap by 4 nucleotides b3204: ptsN - sugar-specific enzyme IIA component of PTS (NCBI), at 3,344,600 to 3,345,091 ptsN b3205: yhbJ - hypothetical protein (NCBI), at 3,345,137 to 3,345,991 yhbJ b3206: npr - phosphohistidinoprotein-hexose phosphotransferase component of N-regulated PTS system (Npr) (NCBI), at 3,345,988 to 3,346,260 npr b3207: yrbL - hypothetical protein (NCBI), at 3,346,474 to 3,347,106 yrbL b3208: mtgA - monofunctional biosynthetic peptidoglycan transglycosylase (NCBI), at 3,347,103 to 3,347,831 mtgA Position (kb) 3345 3346 3347Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3345.026 kb on + strand, within ptsNat 3345.026 kb on + strand, within ptsNat 3345.026 kb on + strand, within ptsNat 3345.034 kb on - strand, within ptsNat 3345.034 kb on - strand, within ptsNat 3345.034 kb on - strand, within ptsNat 3345.034 kb on - strand, within ptsNat 3345.066 kb on + strandat 3345.072 kb on + strandat 3345.072 kb on + strandat 3345.102 kb on - strandat 3345.102 kb on - strandat 3345.102 kb on - strandat 3345.116 kb on - strandat 3345.116 kb on - strandat 3345.139 kb on - strandat 3345.140 kb on + strandat 3345.231 kb on + strand, within yhbJat 3345.249 kb on - strand, within yhbJat 3345.264 kb on - strand, within yhbJat 3345.264 kb on - strand, within yhbJat 3345.305 kb on - strand, within yhbJat 3345.343 kb on + strand, within yhbJat 3345.396 kb on - strand, within yhbJat 3345.698 kb on + strand, within yhbJat 3345.698 kb on + strand, within yhbJat 3345.735 kb on + strand, within yhbJat 3345.863 kb on - strand, within yhbJat 3345.863 kb on - strand, within yhbJat 3345.892 kb on - strand, within yhbJat 3345.892 kb on - strand, within yhbJat 3345.909 kb on - strandat 3345.953 kb on + strandat 3346.034 kb on - strand, within nprat 3346.147 kb on + strand, within nprat 3346.173 kb on - strand, within nprat 3346.174 kb on + strand, within nprat 3346.174 kb on + strand, within nprat 3346.186 kb on - strand, within nprat 3346.196 kb on - strand, within nprat 3346.286 kb on + strandat 3346.286 kb on + strandat 3346.444 kb on - strandat 3346.487 kb on - strandat 3346.655 kb on + strand, within yrbLat 3346.688 kb on - strand, within yrbLat 3346.697 kb on - strand, within yrbLat 3346.795 kb on + strand, within yrbLat 3346.809 kb on + strand, within yrbLat 3346.921 kb on + strand, within yrbLat 3346.921 kb on + strand, within yrbLat 3346.932 kb on - strand, within yrbLat 3346.965 kb on - strand, within yrbLat 3346.972 kb on + strand, within yrbLat 3346.980 kb on - strand, within yrbLat 3346.980 kb on - strand, within yrbLat 3346.986 kb on + strand, within yrbLat 3346.986 kb on + strand, within yrbLat 3347.046 kb on + strandat 3347.086 kb on - strandat 3347.092 kb on - strandat 3347.111 kb on + strandat 3347.151 kb on - strandat 3347.178 kb on + strand, within mtgAat 3347.178 kb on + strand, within mtgAat 3347.178 kb on + strand, within mtgAat 3347.178 kb on + strand, within mtgAat 3347.184 kb on + strand, within mtgAat 3347.192 kb on - strand, within mtgAat 3347.205 kb on - strand, within mtgAat 3347.205 kb on - strand, within mtgAat 3347.226 kb on + strand, within mtgAat 3347.226 kb on + strand, within mtgA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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3,345,026 + ptsN b3204 0.87 -2.9
3,345,026 + ptsN b3204 0.87 -1.8
3,345,026 + ptsN b3204 0.87 -3.8
3,345,034 - ptsN b3204 0.88 -2.6
3,345,034 - ptsN b3204 0.88 -1.0
3,345,034 - ptsN b3204 0.88 -1.4
3,345,034 - ptsN b3204 0.88 -2.4
3,345,066 + -2.8
3,345,072 + -1.2
3,345,072 + -0.7
3,345,102 - -2.5
3,345,102 - -0.6
3,345,102 - -0.7
3,345,116 - -1.7
3,345,116 - -0.8
3,345,139 - -1.0
3,345,140 + -0.3
3,345,231 + yhbJ b3205 0.11 +0.6
3,345,249 - yhbJ b3205 0.13 -0.6
3,345,264 - yhbJ b3205 0.15 +0.6
3,345,264 - yhbJ b3205 0.15 -0.2
3,345,305 - yhbJ b3205 0.20 -2.7
3,345,343 + yhbJ b3205 0.24 +0.3
3,345,396 - yhbJ b3205 0.30 -0.1
3,345,698 + yhbJ b3205 0.66 +0.2
3,345,698 + yhbJ b3205 0.66 -0.4
3,345,735 + yhbJ b3205 0.70 -1.1
3,345,863 - yhbJ b3205 0.85 -0.8
3,345,863 - yhbJ b3205 0.85 -0.1
3,345,892 - yhbJ b3205 0.88 -1.3
3,345,892 - yhbJ b3205 0.88 -0.5
3,345,909 - -1.4
3,345,953 + -0.2
3,346,034 - npr b3206 0.17 +0.6
3,346,147 + npr b3206 0.58 +0.3
3,346,173 - npr b3206 0.68 +0.3
3,346,174 + npr b3206 0.68 +0.6
3,346,174 + npr b3206 0.68 +0.2
3,346,186 - npr b3206 0.73 +0.4
3,346,196 - npr b3206 0.76 +1.1
3,346,286 + +0.9
3,346,286 + +0.6
3,346,444 - -0.2
3,346,487 - -0.2
3,346,655 + yrbL b3207 0.29 -0.3
3,346,688 - yrbL b3207 0.34 +0.1
3,346,697 - yrbL b3207 0.35 -0.4
3,346,795 + yrbL b3207 0.51 +0.5
3,346,809 + yrbL b3207 0.53 +0.1
3,346,921 + yrbL b3207 0.71 -1.5
3,346,921 + yrbL b3207 0.71 +0.7
3,346,932 - yrbL b3207 0.72 -0.1
3,346,965 - yrbL b3207 0.78 -0.5
3,346,972 + yrbL b3207 0.79 -0.1
3,346,980 - yrbL b3207 0.80 +1.0
3,346,980 - yrbL b3207 0.80 +0.8
3,346,986 + yrbL b3207 0.81 +0.1
3,346,986 + yrbL b3207 0.81 -0.7
3,347,046 + +0.2
3,347,086 - +0.2
3,347,092 - -0.2
3,347,111 + +1.0
3,347,151 - -0.4
3,347,178 + mtgA b3208 0.10 -0.4
3,347,178 + mtgA b3208 0.10 +0.2
3,347,178 + mtgA b3208 0.10 +1.6
3,347,178 + mtgA b3208 0.10 +0.7
3,347,184 + mtgA b3208 0.11 +0.2
3,347,192 - mtgA b3208 0.12 -0.5
3,347,205 - mtgA b3208 0.14 -0.3
3,347,205 - mtgA b3208 0.14 +1.2
3,347,226 + mtgA b3208 0.17 +0.8
3,347,226 + mtgA b3208 0.17 -1.8

Or see this region's nucleotide sequence