Strain Fitness in Escherichia coli BW25113 around b2171

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsetB and yeiW overlap by 4 nucleotidesyeiW and yeiP are separated by 154 nucleotidesyeiP and yeiQ are separated by 222 nucleotides b2170: setB - lactose/glucose efflux system (NCBI), at 2,261,885 to 2,263,066 setB b4502: yeiW - hypothetical protein (NCBI), at 2,263,063 to 2,263,317 yeiW b2171: yeiP - putative elongation factor (VIMSS), at 2,263,472 to 2,264,044 yeiP b2172: yeiQ - predicted dehydrogenase, NAD-dependent (NCBI), at 2,264,267 to 2,265,733 yeiQ Position (kb) 2263 2264 2265Strain fitness (log2 ratio) -1 0 1at 2262.590 kb on - strand, within setBat 2262.590 kb on - strand, within setBat 2262.752 kb on - strand, within setBat 2262.916 kb on + strand, within setBat 2262.925 kb on - strand, within setBat 2263.022 kb on + strandat 2263.022 kb on + strandat 2263.060 kb on + strandat 2263.088 kb on - strandat 2263.102 kb on - strand, within yeiWat 2263.102 kb on - strand, within yeiWat 2263.129 kb on + strand, within yeiWat 2263.136 kb on + strand, within yeiWat 2263.213 kb on - strand, within yeiWat 2263.213 kb on - strand, within yeiWat 2263.217 kb on + strand, within yeiWat 2263.286 kb on - strand, within yeiWat 2263.321 kb on - strandat 2263.563 kb on + strand, within yeiPat 2263.563 kb on + strand, within yeiPat 2263.575 kb on + strand, within yeiPat 2263.743 kb on - strand, within yeiPat 2263.813 kb on + strand, within yeiPat 2263.826 kb on + strand, within yeiPat 2263.826 kb on + strand, within yeiPat 2263.908 kb on - strand, within yeiPat 2263.929 kb on - strand, within yeiPat 2263.960 kb on - strand, within yeiPat 2264.034 kb on - strandat 2264.039 kb on + strandat 2264.174 kb on - strandat 2264.184 kb on - strandat 2264.228 kb on - strandat 2264.233 kb on + strandat 2264.272 kb on - strandat 2264.316 kb on - strandat 2264.316 kb on - strandat 2264.482 kb on - strand, within yeiQat 2264.482 kb on - strand, within yeiQat 2264.497 kb on + strand, within yeiQat 2264.497 kb on + strand, within yeiQat 2264.497 kb on + strand, within yeiQat 2264.505 kb on - strand, within yeiQat 2264.565 kb on + strand, within yeiQat 2264.565 kb on + strand, within yeiQat 2264.672 kb on - strand, within yeiQat 2264.946 kb on + strand, within yeiQat 2264.946 kb on + strand, within yeiQat 2265.000 kb on - strand, within yeiQat 2265.000 kb on - strand, within yeiQat 2265.002 kb on - strand, within yeiQ

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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2,262,590 - setB b2170 0.60 -0.2
2,262,590 - setB b2170 0.60 -0.0
2,262,752 - setB b2170 0.73 -0.2
2,262,916 + setB b2170 0.87 +0.0
2,262,925 - setB b2170 0.88 -0.1
2,263,022 + -0.6
2,263,022 + +0.1
2,263,060 + -0.6
2,263,088 - -0.2
2,263,102 - yeiW b4502 0.15 +0.0
2,263,102 - yeiW b4502 0.15 +0.3
2,263,129 + yeiW b4502 0.26 -0.0
2,263,136 + yeiW b4502 0.29 -0.5
2,263,213 - yeiW b4502 0.59 -0.2
2,263,213 - yeiW b4502 0.59 +0.7
2,263,217 + yeiW b4502 0.60 +0.2
2,263,286 - yeiW b4502 0.87 -0.2
2,263,321 - +0.3
2,263,563 + yeiP b2171 0.16 +1.4
2,263,563 + yeiP b2171 0.16 +0.9
2,263,575 + yeiP b2171 0.18 +0.4
2,263,743 - yeiP b2171 0.47 +0.5
2,263,813 + yeiP b2171 0.60 +0.1
2,263,826 + yeiP b2171 0.62 +0.7
2,263,826 + yeiP b2171 0.62 +0.6
2,263,908 - yeiP b2171 0.76 +1.1
2,263,929 - yeiP b2171 0.80 +0.3
2,263,960 - yeiP b2171 0.85 +0.6
2,264,034 - +1.0
2,264,039 + +0.3
2,264,174 - -0.3
2,264,184 - +0.4
2,264,228 - -0.2
2,264,233 + +0.1
2,264,272 - -0.5
2,264,316 - +0.8
2,264,316 - +0.1
2,264,482 - yeiQ b2172 0.15 +0.0
2,264,482 - yeiQ b2172 0.15 +0.3
2,264,497 + yeiQ b2172 0.16 -1.4
2,264,497 + yeiQ b2172 0.16 -0.6
2,264,497 + yeiQ b2172 0.16 -0.4
2,264,505 - yeiQ b2172 0.16 +0.2
2,264,565 + yeiQ b2172 0.20 +0.5
2,264,565 + yeiQ b2172 0.20 -0.3
2,264,672 - yeiQ b2172 0.28 -0.2
2,264,946 + yeiQ b2172 0.46 +0.1
2,264,946 + yeiQ b2172 0.46 +0.6
2,265,000 - yeiQ b2172 0.50 -0.4
2,265,000 - yeiQ b2172 0.50 +0.4
2,265,002 - yeiQ b2172 0.50 +0.0

Or see this region's nucleotide sequence