Strain Fitness in Escherichia coli BW25113 around b1950

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliP and fliQ are separated by 9 nucleotidesfliQ and fliR are separated by 7 nucleotidesfliR and rcsA are separated by 289 nucleotidesrcsA and dsrB are separated by 43 nucleotides b1948: fliP - flagellar biosynthesis protein P (NCBI), at 2,019,893 to 2,020,630 fliP b1949: fliQ - flagellar biosynthesis protein Q (NCBI), at 2,020,640 to 2,020,909 fliQ b1950: fliR - flagellar biosynthesis protein R (NCBI), at 2,020,917 to 2,021,702 fliR b1951: rcsA - DNA-binding transcriptional activator, co-regulator with RcsB (NCBI), at 2,021,992 to 2,022,615 rcsA b1952: dsrB - hypothetical protein (NCBI), at 2,022,659 to 2,022,847 dsrB Position (kb) 2020 2021 2022Strain fitness (log2 ratio) -2 -1 0 1 2at 2019.995 kb on + strand, within fliPat 2020.000 kb on + strand, within fliPat 2020.231 kb on - strand, within fliPat 2020.359 kb on - strand, within fliPat 2020.359 kb on - strand, within fliPat 2020.375 kb on + strand, within fliPat 2020.382 kb on - strand, within fliPat 2020.668 kb on + strand, within fliQat 2020.719 kb on - strand, within fliQat 2020.840 kb on + strand, within fliQat 2020.894 kb on + strandat 2020.899 kb on - strandat 2020.950 kb on - strandat 2021.298 kb on + strand, within fliRat 2021.377 kb on + strand, within fliRat 2021.377 kb on + strand, within fliRat 2021.413 kb on - strand, within fliRat 2021.498 kb on + strand, within fliRat 2021.501 kb on + strand, within fliRat 2021.501 kb on + strand, within fliRat 2021.522 kb on - strand, within fliRat 2021.572 kb on + strand, within fliRat 2021.594 kb on + strand, within fliRat 2021.594 kb on + strand, within fliRat 2021.595 kb on + strand, within fliRat 2021.595 kb on + strand, within fliRat 2021.812 kb on - strandat 2021.982 kb on + strandat 2021.982 kb on + strandat 2022.049 kb on + strandat 2022.191 kb on + strand, within rcsAat 2022.382 kb on - strand, within rcsAat 2022.382 kb on - strand, within rcsAat 2022.413 kb on - strand, within rcsAat 2022.413 kb on - strand, within rcsAat 2022.424 kb on - strand, within rcsAat 2022.424 kb on - strand, within rcsAat 2022.427 kb on - strand, within rcsAat 2022.435 kb on + strand, within rcsAat 2022.453 kb on - strand, within rcsAat 2022.456 kb on - strand, within rcsAat 2022.610 kb on + strandat 2022.610 kb on + strandat 2022.627 kb on - strandat 2022.637 kb on - strandat 2022.650 kb on - strandat 2022.650 kb on - strandat 2022.674 kb on - strandat 2022.674 kb on - strandat 2022.694 kb on + strand, within dsrBat 2022.694 kb on + strand, within dsrB

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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2,019,995 + fliP b1948 0.14 +0.0
2,020,000 + fliP b1948 0.14 +0.0
2,020,231 - fliP b1948 0.46 -0.1
2,020,359 - fliP b1948 0.63 +0.9
2,020,359 - fliP b1948 0.63 +0.2
2,020,375 + fliP b1948 0.65 +1.3
2,020,382 - fliP b1948 0.66 -0.1
2,020,668 + fliQ b1949 0.10 +0.6
2,020,719 - fliQ b1949 0.29 +0.0
2,020,840 + fliQ b1949 0.74 -0.7
2,020,894 + -0.1
2,020,899 - -0.7
2,020,950 - +0.7
2,021,298 + fliR b1950 0.48 +0.6
2,021,377 + fliR b1950 0.59 +0.8
2,021,377 + fliR b1950 0.59 -2.0
2,021,413 - fliR b1950 0.63 +0.5
2,021,498 + fliR b1950 0.74 -1.4
2,021,501 + fliR b1950 0.74 +0.5
2,021,501 + fliR b1950 0.74 -0.4
2,021,522 - fliR b1950 0.77 +2.2
2,021,572 + fliR b1950 0.83 +2.6
2,021,594 + fliR b1950 0.86 +2.6
2,021,594 + fliR b1950 0.86 +0.2
2,021,595 + fliR b1950 0.86 +0.6
2,021,595 + fliR b1950 0.86 +1.4
2,021,812 - +2.2
2,021,982 + +2.2
2,021,982 + +2.2
2,022,049 + -0.1
2,022,191 + rcsA b1951 0.32 +0.6
2,022,382 - rcsA b1951 0.62 +2.2
2,022,382 - rcsA b1951 0.62 +0.2
2,022,413 - rcsA b1951 0.67 -1.5
2,022,413 - rcsA b1951 0.67 +1.6
2,022,424 - rcsA b1951 0.69 +1.2
2,022,424 - rcsA b1951 0.69 -0.4
2,022,427 - rcsA b1951 0.70 +1.5
2,022,435 + rcsA b1951 0.71 +2.2
2,022,453 - rcsA b1951 0.74 +1.4
2,022,456 - rcsA b1951 0.74 -1.1
2,022,610 + +1.2
2,022,610 + +0.3
2,022,627 - -0.1
2,022,637 - +0.1
2,022,650 - +0.0
2,022,650 - +0.4
2,022,674 - -1.5
2,022,674 - -0.3
2,022,694 + dsrB b1952 0.19 +1.5
2,022,694 + dsrB b1952 0.19 -0.3

Or see this region's nucleotide sequence