Strain Fitness in Escherichia coli BW25113 around b1398

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpaaJ and paaK are separated by 26 nucleotidespaaK and paaX are separated by 100 nucleotides b1397: paaJ - acetyl-CoA acetyltransferase (NCBI), at 1,458,917 to 1,460,122 paaJ b1398: paaK - phenylacetyl-CoA ligase (NCBI), at 1,460,149 to 1,461,462 paaK b1399: paaX - DNA-binding transcriptional repressor of phenylacetic acid degradation, aryl-CoA responsive (NCBI), at 1,461,563 to 1,462,513 paaX Position (kb) 1460 1461 1462Strain fitness (log2 ratio) -1 0 1at 1459.188 kb on + strand, within paaJat 1459.188 kb on + strand, within paaJat 1459.425 kb on + strand, within paaJat 1459.425 kb on + strand, within paaJat 1459.608 kb on + strand, within paaJat 1459.647 kb on + strandat 1459.698 kb on + strand, within paaJat 1459.778 kb on - strand, within paaJat 1459.803 kb on + strand, within paaJat 1459.882 kb on + strand, within paaJat 1459.898 kb on + strand, within paaJat 1459.916 kb on - strand, within paaJat 1459.976 kb on + strand, within paaJat 1460.080 kb on - strandat 1460.092 kb on + strandat 1461.222 kb on - strand, within paaKat 1461.441 kb on + strandat 1461.562 kb on + strandat 1461.562 kb on + strandat 1461.638 kb on + strandat 1461.687 kb on + strand, within paaXat 1461.873 kb on + strand, within paaXat 1461.873 kb on + strand, within paaXat 1461.938 kb on + strand, within paaXat 1461.978 kb on + strand, within paaXat 1462.399 kb on - strand, within paaX

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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1,459,188 + paaJ b1397 0.22 -0.3
1,459,188 + paaJ b1397 0.22 -0.7
1,459,425 + paaJ b1397 0.42 -0.2
1,459,425 + paaJ b1397 0.42 -0.6
1,459,608 + paaJ b1397 0.57 -0.3
1,459,647 + +0.5
1,459,698 + paaJ b1397 0.65 -0.7
1,459,778 - paaJ b1397 0.71 -0.1
1,459,803 + paaJ b1397 0.73 -0.2
1,459,882 + paaJ b1397 0.80 -1.7
1,459,898 + paaJ b1397 0.81 -1.2
1,459,916 - paaJ b1397 0.83 -0.6
1,459,976 + paaJ b1397 0.88 +0.3
1,460,080 - -0.2
1,460,092 + -0.1
1,461,222 - paaK b1398 0.82 +0.7
1,461,441 + -0.5
1,461,562 + -0.4
1,461,562 + +0.2
1,461,638 + +0.3
1,461,687 + paaX b1399 0.13 -0.6
1,461,873 + paaX b1399 0.33 +0.5
1,461,873 + paaX b1399 0.33 -0.8
1,461,938 + paaX b1399 0.39 +1.3
1,461,978 + paaX b1399 0.44 +0.1
1,462,399 - paaX b1399 0.88 +0.1

Or see this region's nucleotide sequence