Strain Fitness in Escherichia coli BW25113 around b0078

Experiment: Ying_OrganicAcid7 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntilvI and ilvH are separated by 2 nucleotidesilvH and fruR are separated by 179 nucleotides b0077: ilvI - acetolactate synthase III large subunit (NCBI), at 85,630 to 87,354 ilvI b0078: ilvH - acetolactate synthase small subunit (NCBI), at 87,357 to 87,848 ilvH b0080: fruR - DNA-binding transcriptional dual regulator (NCBI), at 88,028 to 89,032 fruR Position (kb) 87 88Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 86.357 kb on + strand, within ilvIat 86.357 kb on + strand, within ilvIat 86.366 kb on - strand, within ilvIat 86.367 kb on + strand, within ilvIat 86.438 kb on + strand, within ilvIat 86.447 kb on - strand, within ilvIat 86.447 kb on - strand, within ilvIat 86.508 kb on + strand, within ilvIat 86.517 kb on + strand, within ilvIat 86.535 kb on - strand, within ilvIat 86.535 kb on - strand, within ilvIat 86.548 kb on - strand, within ilvIat 86.593 kb on - strand, within ilvIat 86.602 kb on + strand, within ilvIat 86.602 kb on + strand, within ilvIat 86.797 kb on + strand, within ilvIat 86.841 kb on + strand, within ilvIat 86.894 kb on - strand, within ilvIat 86.899 kb on + strand, within ilvIat 87.001 kb on - strand, within ilvIat 87.026 kb on + strand, within ilvIat 87.034 kb on - strand, within ilvIat 87.062 kb on - strand, within ilvIat 87.070 kb on + strand, within ilvIat 87.070 kb on + strand, within ilvIat 87.092 kb on - strand, within ilvIat 87.098 kb on - strand, within ilvIat 87.098 kb on - strand, within ilvIat 87.130 kb on + strand, within ilvIat 87.165 kb on - strand, within ilvIat 87.166 kb on - strand, within ilvIat 87.250 kb on + strandat 87.285 kb on + strandat 87.443 kb on - strand, within ilvHat 87.480 kb on + strand, within ilvHat 87.497 kb on + strand, within ilvHat 87.571 kb on + strand, within ilvHat 87.571 kb on + strand, within ilvHat 87.588 kb on - strand, within ilvHat 87.617 kb on - strand, within ilvHat 87.617 kb on - strand, within ilvHat 87.640 kb on + strand, within ilvHat 87.665 kb on - strand, within ilvHat 87.698 kb on - strand, within ilvHat 87.705 kb on + strand, within ilvHat 87.710 kb on + strand, within ilvHat 87.710 kb on + strand, within ilvHat 87.718 kb on - strand, within ilvHat 87.718 kb on - strand, within ilvHat 87.718 kb on - strand, within ilvHat 87.718 kb on - strand, within ilvHat 87.740 kb on - strand, within ilvHat 87.744 kb on - strand, within ilvHat 87.785 kb on + strand, within ilvHat 87.802 kb on + strandat 87.815 kb on - strandat 87.918 kb on - strandat 87.951 kb on - strandat 87.955 kb on + strandat 88.009 kb on + strandat 88.074 kb on + strandat 88.085 kb on + strandat 88.111 kb on - strandat 88.111 kb on - strandat 88.111 kb on - strandat 88.111 kb on - strandat 88.113 kb on - strandat 88.187 kb on + strand, within fruRat 88.187 kb on + strand, within fruRat 88.259 kb on - strand, within fruRat 88.265 kb on + strand, within fruRat 88.365 kb on + strand, within fruRat 88.365 kb on + strand, within fruRat 88.432 kb on + strand, within fruRat 88.432 kb on + strand, within fruRat 88.471 kb on - strand, within fruRat 88.482 kb on + strand, within fruRat 88.490 kb on - strand, within fruRat 88.492 kb on + strand, within fruRat 88.492 kb on + strand, within fruRat 88.492 kb on + strand, within fruRat 88.500 kb on - strand, within fruRat 88.517 kb on - strand, within fruRat 88.517 kb on - strand, within fruRat 88.683 kb on + strand, within fruRat 88.753 kb on + strand, within fruRat 88.772 kb on + strandat 88.774 kb on + strand, within fruRat 88.821 kb on + strand, within fruR

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_OrganicAcid7 rep B; time point 2
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86,357 + ilvI b0077 0.42 -3.8
86,357 + ilvI b0077 0.42 -3.2
86,366 - ilvI b0077 0.43 -3.8
86,367 + ilvI b0077 0.43 -5.6
86,438 + ilvI b0077 0.47 -1.2
86,447 - ilvI b0077 0.47 -2.1
86,447 - ilvI b0077 0.47 -2.6
86,508 + ilvI b0077 0.51 -4.3
86,517 + ilvI b0077 0.51 -4.7
86,535 - ilvI b0077 0.52 -4.6
86,535 - ilvI b0077 0.52 -4.8
86,548 - ilvI b0077 0.53 -5.6
86,593 - ilvI b0077 0.56 -4.6
86,602 + ilvI b0077 0.56 -4.0
86,602 + ilvI b0077 0.56 -4.2
86,797 + ilvI b0077 0.68 -4.7
86,841 + ilvI b0077 0.70 -3.1
86,894 - ilvI b0077 0.73 -3.3
86,899 + ilvI b0077 0.74 -4.5
87,001 - ilvI b0077 0.79 -6.2
87,026 + ilvI b0077 0.81 -2.2
87,034 - ilvI b0077 0.81 -3.2
87,062 - ilvI b0077 0.83 -5.0
87,070 + ilvI b0077 0.83 -5.0
87,070 + ilvI b0077 0.83 -3.5
87,092 - ilvI b0077 0.85 -4.7
87,098 - ilvI b0077 0.85 -2.5
87,098 - ilvI b0077 0.85 -4.7
87,130 + ilvI b0077 0.87 -5.1
87,165 - ilvI b0077 0.89 -5.8
87,166 - ilvI b0077 0.89 -2.4
87,250 + -3.8
87,285 + -2.3
87,443 - ilvH b0078 0.17 -1.4
87,480 + ilvH b0078 0.25 -5.1
87,497 + ilvH b0078 0.28 -6.3
87,571 + ilvH b0078 0.43 -4.1
87,571 + ilvH b0078 0.43 -4.3
87,588 - ilvH b0078 0.47 -3.5
87,617 - ilvH b0078 0.53 -3.1
87,617 - ilvH b0078 0.53 -2.7
87,640 + ilvH b0078 0.58 -5.7
87,665 - ilvH b0078 0.63 +0.2
87,698 - ilvH b0078 0.69 -2.5
87,705 + ilvH b0078 0.71 -4.1
87,710 + ilvH b0078 0.72 -4.3
87,710 + ilvH b0078 0.72 -3.5
87,718 - ilvH b0078 0.73 -5.6
87,718 - ilvH b0078 0.73 -4.2
87,718 - ilvH b0078 0.73 -3.6
87,718 - ilvH b0078 0.73 -3.7
87,740 - ilvH b0078 0.78 -5.6
87,744 - ilvH b0078 0.79 -4.0
87,785 + ilvH b0078 0.87 -5.2
87,802 + -4.8
87,815 - -4.2
87,918 - -1.4
87,951 - -2.9
87,955 + -0.3
88,009 + -0.7
88,074 + -6.1
88,085 + -1.4
88,111 - -1.9
88,111 - -1.7
88,111 - -2.1
88,111 - -2.1
88,113 - -4.7
88,187 + fruR b0080 0.16 -2.8
88,187 + fruR b0080 0.16 -3.0
88,259 - fruR b0080 0.23 -4.2
88,265 + fruR b0080 0.24 -3.6
88,365 + fruR b0080 0.34 -3.9
88,365 + fruR b0080 0.34 -3.6
88,432 + fruR b0080 0.40 -2.7
88,432 + fruR b0080 0.40 -2.5
88,471 - fruR b0080 0.44 -3.2
88,482 + fruR b0080 0.45 -3.7
88,490 - fruR b0080 0.46 -2.0
88,492 + fruR b0080 0.46 -1.6
88,492 + fruR b0080 0.46 -1.8
88,492 + fruR b0080 0.46 -4.3
88,500 - fruR b0080 0.47 -3.7
88,517 - fruR b0080 0.49 -2.1
88,517 - fruR b0080 0.49 -3.0
88,683 + fruR b0080 0.65 -3.2
88,753 + fruR b0080 0.72 -4.8
88,772 + +0.3
88,774 + fruR b0080 0.74 -3.3
88,821 + fruR b0080 0.79 -3.9

Or see this region's nucleotide sequence