Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhuF and yjjZ are separated by 140 nucleotides yjjZ and leuV are separated by 38 nucleotides leuV and leuP are separated by 34 nucleotides leuP and leuQ are separated by 28 nucleotides leuQ and rsmC are separated by 267 nucleotides
b4367: fhuF - ferric iron reductase involved in ferric hydroximate transport (NCBI), at 4,602,898 to 4,603,686
fhuF
b4567: yjjZ - hypothetical protein (NCBI), at 4,603,827 to 4,604,063
yjjZ
b4368: leuV - tRNA-Leu (NCBI), at 4,604,102 to 4,604,188
leuV
b4369: leuP - tRNA-Leu (NCBI), at 4,604,223 to 4,604,309
leuP
b4370: leuQ - tRNA-Leu (NCBI), at 4,604,338 to 4,604,424
leuQ
b4371: rsmC - 16S ribosomal RNA m2G1207 methyltransferase (NCBI), at 4,604,692 to 4,605,723
rsmC
Position (kb) 4604
4605 Strain fitness (log2 ratio) -1
0
1 at 4603.360 kb on - strand, within fhuF at 4603.369 kb on + strand, within fhuF at 4603.385 kb on - strand, within fhuF at 4603.385 kb on - strand, within fhuF at 4603.388 kb on + strand, within fhuF at 4603.388 kb on + strand, within fhuF at 4603.393 kb on + strand, within fhuF at 4603.405 kb on + strand, within fhuF at 4603.519 kb on + strand, within fhuF at 4603.847 kb on - strand at 4603.872 kb on + strand, within yjjZ at 4603.882 kb on + strand, within yjjZ at 4603.927 kb on - strand, within yjjZ at 4603.927 kb on - strand, within yjjZ at 4603.935 kb on - strand, within yjjZ at 4603.937 kb on + strand, within yjjZ at 4604.119 kb on - strand, within leuV at 4604.280 kb on - strand, within leuP at 4604.280 kb on - strand, within leuP at 4604.377 kb on - strand, within leuQ at 4604.377 kb on - strand, within leuQ at 4604.381 kb on - strand, within leuQ at 4604.387 kb on + strand, within leuQ at 4604.410 kb on + strand, within leuQ at 4604.467 kb on + strand at 4604.467 kb on + strand at 4604.467 kb on + strand at 4604.508 kb on - strand at 4604.618 kb on - strand at 4604.653 kb on - strand at 4604.732 kb on + strand at 4604.732 kb on + strand at 4604.745 kb on + strand at 4604.758 kb on + strand at 4604.851 kb on + strand, within rsmC at 4604.856 kb on + strand, within rsmC at 4604.884 kb on + strand, within rsmC at 4604.884 kb on + strand, within rsmC at 4604.927 kb on - strand, within rsmC at 4604.927 kb on - strand, within rsmC at 4605.041 kb on + strand, within rsmC at 4605.138 kb on + strand, within rsmC at 4605.178 kb on + strand, within rsmC at 4605.238 kb on + strand, within rsmC at 4605.238 kb on + strand, within rsmC at 4605.298 kb on - strand, within rsmC at 4605.304 kb on + strand, within rsmC at 4605.407 kb on + strand, within rsmC
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 4,603,360 - fhuF b4367 0.59 +0.5 4,603,369 + fhuF b4367 0.60 +0.7 4,603,385 - fhuF b4367 0.62 -0.0 4,603,385 - fhuF b4367 0.62 -0.7 4,603,388 + fhuF b4367 0.62 -0.1 4,603,388 + fhuF b4367 0.62 +0.1 4,603,393 + fhuF b4367 0.63 +0.2 4,603,405 + fhuF b4367 0.64 +0.7 4,603,519 + fhuF b4367 0.79 -0.3 4,603,847 - +0.2 4,603,872 + yjjZ b4567 0.19 +0.2 4,603,882 + yjjZ b4567 0.23 +0.3 4,603,927 - yjjZ b4567 0.42 +0.1 4,603,927 - yjjZ b4567 0.42 -0.1 4,603,935 - yjjZ b4567 0.46 +0.3 4,603,937 + yjjZ b4567 0.46 +0.4 4,604,119 - leuV b4368 0.20 +0.4 4,604,280 - leuP b4369 0.66 +0.1 4,604,280 - leuP b4369 0.66 -0.3 4,604,377 - leuQ b4370 0.45 -0.1 4,604,377 - leuQ b4370 0.45 +0.4 4,604,381 - leuQ b4370 0.49 +0.3 4,604,387 + leuQ b4370 0.56 +1.5 4,604,410 + leuQ b4370 0.83 +0.1 4,604,467 + +1.4 4,604,467 + +0.0 4,604,467 + -0.6 4,604,508 - +0.0 4,604,618 - -0.1 4,604,653 - +0.1 4,604,732 + +0.1 4,604,732 + -0.8 4,604,745 + +0.4 4,604,758 + +0.1 4,604,851 + rsmC b4371 0.15 +0.4 4,604,856 + rsmC b4371 0.16 -0.3 4,604,884 + rsmC b4371 0.19 +0.3 4,604,884 + rsmC b4371 0.19 +0.3 4,604,927 - rsmC b4371 0.23 -0.0 4,604,927 - rsmC b4371 0.23 -0.0 4,605,041 + rsmC b4371 0.34 -0.6 4,605,138 + rsmC b4371 0.43 -0.2 4,605,178 + rsmC b4371 0.47 -0.5 4,605,238 + rsmC b4371 0.53 -0.6 4,605,238 + rsmC b4371 0.53 -0.1 4,605,298 - rsmC b4371 0.59 +0.1 4,605,304 + rsmC b4371 0.59 +0.0 4,605,407 + rsmC b4371 0.69 -0.4
Or see this region's nucleotide sequence