Strain Fitness in Escherichia coli BW25113 around b2661

Experiment: Ying_AminoAcid20 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntygaF and gabD are separated by 22 nucleotidesgabD and gabT are separated by 13 nucleotides b2660: ygaF - orf, hypothetical protein (VIMSS), at 2,788,004 to 2,789,272 ygaF b2661: gabD - succinate-semialdehyde dehydrogenase I, NADP-dependent (NCBI), at 2,789,295 to 2,790,743 gabD b2662: gabT - 4-aminobutyrate aminotransferase (NCBI), at 2,790,757 to 2,792,037 gabT Position (kb) 2789 2790 2791Strain fitness (log2 ratio) -1 0 1at 2788.322 kb on - strand, within ygaFat 2788.324 kb on - strand, within ygaFat 2788.324 kb on - strand, within ygaFat 2788.349 kb on - strand, within ygaFat 2788.551 kb on + strand, within ygaFat 2788.551 kb on + strand, within ygaFat 2788.665 kb on - strand, within ygaFat 2788.753 kb on - strand, within ygaFat 2788.753 kb on - strand, within ygaFat 2788.861 kb on + strand, within ygaFat 2788.861 kb on + strand, within ygaFat 2788.931 kb on - strand, within ygaFat 2789.116 kb on - strand, within ygaFat 2789.211 kb on - strandat 2789.230 kb on + strandat 2789.230 kb on + strandat 2789.254 kb on - strandat 2789.254 kb on - strandat 2789.309 kb on - strandat 2789.344 kb on - strandat 2789.436 kb on + strandat 2789.699 kb on + strand, within gabDat 2789.739 kb on - strand, within gabDat 2789.739 kb on - strand, within gabDat 2789.765 kb on - strand, within gabDat 2789.952 kb on - strand, within gabDat 2790.018 kb on + strand, within gabDat 2790.018 kb on + strand, within gabDat 2790.034 kb on - strand, within gabDat 2790.099 kb on + strand, within gabDat 2790.099 kb on + strand, within gabDat 2790.123 kb on + strand, within gabDat 2790.123 kb on + strand, within gabDat 2790.471 kb on + strand, within gabDat 2790.471 kb on + strand, within gabDat 2790.471 kb on - strand, within gabDat 2790.502 kb on + strand, within gabDat 2790.534 kb on - strand, within gabDat 2790.534 kb on - strand, within gabDat 2790.588 kb on + strand, within gabDat 2790.675 kb on + strandat 2790.922 kb on + strand, within gabTat 2791.013 kb on + strand, within gabTat 2791.077 kb on + strand, within gabTat 2791.085 kb on - strand, within gabTat 2791.085 kb on - strand, within gabTat 2791.088 kb on + strand, within gabTat 2791.183 kb on - strand, within gabTat 2791.183 kb on - strand, within gabTat 2791.223 kb on - strand, within gabTat 2791.324 kb on - strand, within gabTat 2791.454 kb on + strand, within gabTat 2791.462 kb on - strand, within gabTat 2791.487 kb on + strand, within gabTat 2791.509 kb on + strand, within gabTat 2791.532 kb on - strand, within gabTat 2791.597 kb on - strand, within gabTat 2791.597 kb on - strand, within gabTat 2791.688 kb on + strand, within gabT

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2
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2,788,322 - ygaF b2660 0.25 -0.1
2,788,324 - ygaF b2660 0.25 -1.6
2,788,324 - ygaF b2660 0.25 -0.7
2,788,349 - ygaF b2660 0.27 +0.3
2,788,551 + ygaF b2660 0.43 +0.1
2,788,551 + ygaF b2660 0.43 -0.8
2,788,665 - ygaF b2660 0.52 +0.1
2,788,753 - ygaF b2660 0.59 -0.0
2,788,753 - ygaF b2660 0.59 -0.4
2,788,861 + ygaF b2660 0.68 +0.9
2,788,861 + ygaF b2660 0.68 -0.0
2,788,931 - ygaF b2660 0.73 +0.6
2,789,116 - ygaF b2660 0.88 +0.3
2,789,211 - -0.7
2,789,230 + -0.5
2,789,230 + +0.8
2,789,254 - -0.1
2,789,254 - +0.1
2,789,309 - +0.1
2,789,344 - -1.1
2,789,436 + -0.3
2,789,699 + gabD b2661 0.28 -0.1
2,789,739 - gabD b2661 0.31 -0.8
2,789,739 - gabD b2661 0.31 -0.7
2,789,765 - gabD b2661 0.32 -0.4
2,789,952 - gabD b2661 0.45 -0.8
2,790,018 + gabD b2661 0.50 +0.3
2,790,018 + gabD b2661 0.50 +0.4
2,790,034 - gabD b2661 0.51 -0.2
2,790,099 + gabD b2661 0.55 +0.2
2,790,099 + gabD b2661 0.55 -0.4
2,790,123 + gabD b2661 0.57 -0.0
2,790,123 + gabD b2661 0.57 -0.2
2,790,471 + gabD b2661 0.81 -0.5
2,790,471 + gabD b2661 0.81 +0.8
2,790,471 - gabD b2661 0.81 +0.8
2,790,502 + gabD b2661 0.83 +0.3
2,790,534 - gabD b2661 0.86 +0.4
2,790,534 - gabD b2661 0.86 +0.9
2,790,588 + gabD b2661 0.89 -0.0
2,790,675 + +0.2
2,790,922 + gabT b2662 0.13 -0.2
2,791,013 + gabT b2662 0.20 +0.3
2,791,077 + gabT b2662 0.25 -0.0
2,791,085 - gabT b2662 0.26 +0.2
2,791,085 - gabT b2662 0.26 +1.3
2,791,088 + gabT b2662 0.26 +0.9
2,791,183 - gabT b2662 0.33 +1.6
2,791,183 - gabT b2662 0.33 +0.2
2,791,223 - gabT b2662 0.36 +0.1
2,791,324 - gabT b2662 0.44 +0.1
2,791,454 + gabT b2662 0.54 +1.1
2,791,462 - gabT b2662 0.55 -0.6
2,791,487 + gabT b2662 0.57 -0.6
2,791,509 + gabT b2662 0.59 +0.2
2,791,532 - gabT b2662 0.60 -0.8
2,791,597 - gabT b2662 0.66 +0.3
2,791,597 - gabT b2662 0.66 +0.2
2,791,688 + gabT b2662 0.73 +0.5

Or see this region's nucleotide sequence