Strain Fitness in Escherichia coli BW25113 around b2593

Experiment: Ying_AminoAcid20 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntclpB and ryfD overlap by 21 nucleotidesryfD and yfiH are separated by 7 nucleotidesyfiH and rluD overlap by 4 nucleotides b2592: clpB - protein disaggregation chaperone (NCBI), at 2,729,622 to 2,732,195 clpB b4609: ryfD - Novel sRNA, function unknown (RefSeq), at 2,732,175 to 2,732,317 ryfD b2593: yfiH - hypothetical protein (NCBI), at 2,732,325 to 2,733,056 yfiH b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033 rluD Position (kb) 2732 2733 2734Strain fitness (log2 ratio) -1 0 1at 2731.351 kb on + strand, within clpBat 2731.448 kb on - strand, within clpBat 2731.468 kb on + strand, within clpBat 2731.476 kb on - strand, within clpBat 2731.476 kb on - strand, within clpBat 2731.481 kb on - strand, within clpBat 2731.531 kb on - strand, within clpBat 2731.575 kb on - strand, within clpBat 2731.674 kb on - strand, within clpBat 2731.730 kb on + strand, within clpBat 2731.736 kb on - strand, within clpBat 2731.746 kb on - strand, within clpBat 2731.746 kb on - strand, within clpBat 2731.857 kb on - strand, within clpBat 2731.868 kb on + strand, within clpBat 2731.919 kb on + strand, within clpBat 2731.927 kb on - strand, within clpBat 2731.965 kb on - strandat 2731.966 kb on - strandat 2731.966 kb on - strandat 2732.002 kb on - strandat 2732.140 kb on - strandat 2732.202 kb on + strand, within ryfDat 2732.205 kb on - strand, within ryfDat 2732.212 kb on + strand, within ryfDat 2732.212 kb on + strand, within ryfDat 2732.251 kb on + strand, within ryfDat 2732.277 kb on - strand, within ryfDat 2732.277 kb on - strand, within ryfDat 2732.323 kb on + strandat 2732.323 kb on + strandat 2732.456 kb on - strand, within yfiHat 2732.499 kb on - strand, within yfiHat 2732.499 kb on - strand, within yfiHat 2732.502 kb on - strand, within yfiHat 2732.502 kb on - strand, within yfiHat 2732.563 kb on - strand, within yfiHat 2732.563 kb on - strand, within yfiHat 2732.594 kb on + strand, within yfiHat 2732.602 kb on - strand, within yfiHat 2732.648 kb on + strand, within yfiHat 2732.659 kb on + strand, within yfiHat 2732.669 kb on + strand, within yfiHat 2732.748 kb on + strand, within yfiHat 2732.756 kb on - strand, within yfiHat 2732.763 kb on - strand, within yfiHat 2732.763 kb on - strand, within yfiHat 2732.778 kb on + strand, within yfiHat 2732.813 kb on - strand, within yfiHat 2732.813 kb on - strand, within yfiHat 2732.852 kb on - strand, within yfiHat 2732.854 kb on - strand, within yfiHat 2732.926 kb on + strand, within yfiHat 2732.943 kb on - strand, within yfiHat 2732.969 kb on - strand, within yfiHat 2732.969 kb on - strand, within yfiHat 2732.994 kb on + strandat 2733.052 kb on + strandat 2733.060 kb on - strandat 2733.062 kb on - strandat 2733.072 kb on + strandat 2733.079 kb on - strandat 2733.079 kb on - strandat 2734.040 kb on - strandat 2734.040 kb on - strandat 2734.042 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2
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2,731,351 + clpB b2592 0.67 +0.2
2,731,448 - clpB b2592 0.71 -0.2
2,731,468 + clpB b2592 0.72 -0.2
2,731,476 - clpB b2592 0.72 +0.5
2,731,476 - clpB b2592 0.72 +0.1
2,731,481 - clpB b2592 0.72 +0.1
2,731,531 - clpB b2592 0.74 -0.3
2,731,575 - clpB b2592 0.76 -0.1
2,731,674 - clpB b2592 0.80 +0.4
2,731,730 + clpB b2592 0.82 +0.6
2,731,736 - clpB b2592 0.82 -0.7
2,731,746 - clpB b2592 0.83 +0.1
2,731,746 - clpB b2592 0.83 -0.7
2,731,857 - clpB b2592 0.87 -0.0
2,731,868 + clpB b2592 0.87 +0.5
2,731,919 + clpB b2592 0.89 +0.4
2,731,927 - clpB b2592 0.90 -0.4
2,731,965 - -0.7
2,731,966 - +0.6
2,731,966 - +0.6
2,732,002 - +0.2
2,732,140 - +0.3
2,732,202 + ryfD b4609 0.19 -0.6
2,732,205 - ryfD b4609 0.21 +0.7
2,732,212 + ryfD b4609 0.26 +0.1
2,732,212 + ryfD b4609 0.26 +1.1
2,732,251 + ryfD b4609 0.53 +0.2
2,732,277 - ryfD b4609 0.71 -0.2
2,732,277 - ryfD b4609 0.71 -0.1
2,732,323 + +0.2
2,732,323 + -0.5
2,732,456 - yfiH b2593 0.18 +0.0
2,732,499 - yfiH b2593 0.24 -1.4
2,732,499 - yfiH b2593 0.24 -0.4
2,732,502 - yfiH b2593 0.24 -0.8
2,732,502 - yfiH b2593 0.24 +0.4
2,732,563 - yfiH b2593 0.33 +0.4
2,732,563 - yfiH b2593 0.33 +0.7
2,732,594 + yfiH b2593 0.37 +0.3
2,732,602 - yfiH b2593 0.38 +0.3
2,732,648 + yfiH b2593 0.44 -0.4
2,732,659 + yfiH b2593 0.46 +0.2
2,732,669 + yfiH b2593 0.47 +0.5
2,732,748 + yfiH b2593 0.58 -0.3
2,732,756 - yfiH b2593 0.59 -0.2
2,732,763 - yfiH b2593 0.60 +0.4
2,732,763 - yfiH b2593 0.60 +0.1
2,732,778 + yfiH b2593 0.62 +0.3
2,732,813 - yfiH b2593 0.67 +0.5
2,732,813 - yfiH b2593 0.67 -0.1
2,732,852 - yfiH b2593 0.72 +0.1
2,732,854 - yfiH b2593 0.72 +0.3
2,732,926 + yfiH b2593 0.82 +0.2
2,732,943 - yfiH b2593 0.84 +0.9
2,732,969 - yfiH b2593 0.88 +0.8
2,732,969 - yfiH b2593 0.88 +0.2
2,732,994 + +0.3
2,733,052 + +0.5
2,733,060 - +0.2
2,733,062 - -0.0
2,733,072 + +0.5
2,733,079 - -0.9
2,733,079 - -1.2
2,734,040 - -0.3
2,734,040 - +0.1
2,734,042 - +0.2

Or see this region's nucleotide sequence