Strain Fitness in Escherichia coli BW25113 around b1667

Experiment: Ying_AminoAcid20 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhQ and valV are separated by 307 nucleotidesvalV and valW are separated by 4 nucleotidesvalW and ydhR are separated by 107 nucleotidesydhR and ydhS are separated by 125 nucleotides b1664: ydhQ - hypothetical protein (NCBI), at 1,742,895 to 1,744,151 ydhQ b1665: valV - tRNA-Val (NCBI), at 1,744,459 to 1,744,535 valV b1666: valW - tRNA-Val (NCBI), at 1,744,540 to 1,744,616 valW b1667: ydhR - hypothetical protein (NCBI), at 1,744,724 to 1,745,029 ydhR b1668: ydhS - conserved protein with FAD/NAD(P)-binding domain (NCBI), at 1,745,155 to 1,746,759 ydhS Position (kb) 1744 1745 1746Strain fitness (log2 ratio) -1 0 1at 1743.892 kb on + strand, within ydhQat 1744.114 kb on + strandat 1744.176 kb on - strandat 1744.194 kb on - strandat 1744.570 kb on + strand, within valWat 1744.671 kb on - strandat 1744.797 kb on - strand, within ydhRat 1744.953 kb on + strand, within ydhRat 1745.178 kb on - strandat 1745.178 kb on - strandat 1745.272 kb on + strandat 1745.288 kb on + strandat 1745.349 kb on + strand, within ydhSat 1745.351 kb on + strand, within ydhSat 1745.351 kb on + strand, within ydhSat 1745.600 kb on - strand, within ydhSat 1745.646 kb on + strand, within ydhSat 1745.649 kb on - strand, within ydhSat 1745.735 kb on - strand, within ydhSat 1745.752 kb on + strand, within ydhSat 1745.752 kb on + strand, within ydhSat 1745.934 kb on + strand, within ydhS

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2
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1,743,892 + ydhQ b1664 0.79 -0.4
1,744,114 + -0.3
1,744,176 - +0.2
1,744,194 - -0.2
1,744,570 + valW b1666 0.39 +0.5
1,744,671 - +1.0
1,744,797 - ydhR b1667 0.24 +0.6
1,744,953 + ydhR b1667 0.75 +0.3
1,745,178 - +0.3
1,745,178 - +0.6
1,745,272 + -0.2
1,745,288 + +0.3
1,745,349 + ydhS b1668 0.12 -0.3
1,745,351 + ydhS b1668 0.12 -0.3
1,745,351 + ydhS b1668 0.12 -0.0
1,745,600 - ydhS b1668 0.28 +0.3
1,745,646 + ydhS b1668 0.31 +0.4
1,745,649 - ydhS b1668 0.31 -0.8
1,745,735 - ydhS b1668 0.36 -0.7
1,745,752 + ydhS b1668 0.37 -0.2
1,745,752 + ydhS b1668 0.37 +0.4
1,745,934 + ydhS b1668 0.49 +0.1

Or see this region's nucleotide sequence