Strain Fitness in Escherichia coli BW25113 around b1450

Experiment: Ying_AminoAcid20 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyncB and b1450 are separated by 197 nucleotidesb1450 and yncD are separated by 35 nucleotides b1449: yncB - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (RefSeq), at 1,517,051 to 1,518,088 yncB b1450: b1450 - orf, hypothetical protein (VIMSS), at 1,518,286 to 1,518,951 b1450 b1451: yncD - predicted iron outer membrane transporter (NCBI), at 1,518,987 to 1,521,089 yncD Position (kb) 1518 1519Strain fitness (log2 ratio) -1 0 1 2 3at 1517.310 kb on + strand, within yncBat 1517.341 kb on + strand, within yncBat 1517.341 kb on + strand, within yncBat 1517.496 kb on - strand, within yncBat 1517.517 kb on - strand, within yncBat 1517.635 kb on - strand, within yncBat 1517.635 kb on - strand, within yncBat 1517.739 kb on - strand, within yncBat 1517.739 kb on - strand, within yncBat 1517.741 kb on - strand, within yncBat 1517.805 kb on + strand, within yncBat 1517.805 kb on + strand, within yncBat 1517.839 kb on - strand, within yncBat 1517.839 kb on - strand, within yncBat 1517.923 kb on + strand, within yncBat 1517.923 kb on + strand, within yncBat 1517.950 kb on - strand, within yncBat 1517.950 kb on - strand, within yncBat 1518.047 kb on - strandat 1518.065 kb on - strandat 1518.067 kb on - strandat 1518.147 kb on + strandat 1518.175 kb on - strandat 1518.175 kb on - strandat 1518.200 kb on + strandat 1518.225 kb on - strandat 1518.237 kb on + strandat 1518.254 kb on + strandat 1518.295 kb on - strandat 1518.330 kb on + strandat 1518.338 kb on - strandat 1518.349 kb on - strandat 1518.354 kb on + strand, within b1450at 1518.423 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.425 kb on + strand, within b1450at 1518.495 kb on + strand, within b1450at 1518.498 kb on + strand, within b1450at 1518.506 kb on + strand, within b1450at 1518.746 kb on - strand, within b1450at 1518.756 kb on + strand, within b1450at 1518.756 kb on + strand, within b1450at 1518.767 kb on + strand, within b1450at 1518.775 kb on - strand, within b1450at 1518.779 kb on + strand, within b1450at 1518.844 kb on + strand, within b1450at 1518.844 kb on + strand, within b1450at 1518.871 kb on + strand, within b1450at 1518.872 kb on + strand, within b1450at 1518.954 kb on - strandat 1518.974 kb on + strandat 1519.053 kb on - strandat 1519.084 kb on - strandat 1519.089 kb on - strandat 1519.228 kb on - strand, within yncDat 1519.274 kb on - strand, within yncDat 1519.282 kb on - strand, within yncDat 1519.282 kb on - strand, within yncDat 1519.284 kb on - strand, within yncDat 1519.296 kb on + strand, within yncDat 1519.313 kb on + strand, within yncDat 1519.313 kb on + strand, within yncDat 1519.426 kb on + strand, within yncDat 1519.485 kb on - strand, within yncDat 1519.551 kb on - strand, within yncDat 1519.645 kb on + strand, within yncDat 1519.653 kb on - strand, within yncDat 1519.653 kb on - strand, within yncDat 1519.784 kb on - strand, within yncDat 1519.828 kb on + strand, within yncD

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2
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1,517,310 + yncB b1449 0.25 -0.0
1,517,341 + yncB b1449 0.28 -0.2
1,517,341 + yncB b1449 0.28 -0.2
1,517,496 - yncB b1449 0.43 +0.2
1,517,517 - yncB b1449 0.45 -0.0
1,517,635 - yncB b1449 0.56 -0.4
1,517,635 - yncB b1449 0.56 -0.2
1,517,739 - yncB b1449 0.66 -0.2
1,517,739 - yncB b1449 0.66 +0.0
1,517,741 - yncB b1449 0.66 +0.5
1,517,805 + yncB b1449 0.73 -0.8
1,517,805 + yncB b1449 0.73 +0.1
1,517,839 - yncB b1449 0.76 +0.5
1,517,839 - yncB b1449 0.76 +0.1
1,517,923 + yncB b1449 0.84 +0.1
1,517,923 + yncB b1449 0.84 -0.0
1,517,950 - yncB b1449 0.87 +0.4
1,517,950 - yncB b1449 0.87 -0.4
1,518,047 - +1.1
1,518,065 - +2.1
1,518,067 - +2.1
1,518,147 + -0.3
1,518,175 - +1.3
1,518,175 - +0.7
1,518,200 + +0.1
1,518,225 - +1.9
1,518,237 + -0.7
1,518,254 + -0.3
1,518,295 - +0.4
1,518,330 + +0.3
1,518,338 - +0.4
1,518,349 - +2.1
1,518,354 + b1450 b1450 0.10 +1.1
1,518,423 + b1450 b1450 0.21 +0.6
1,518,425 + b1450 b1450 0.21 +1.1
1,518,425 + b1450 b1450 0.21 +0.5
1,518,425 + b1450 b1450 0.21 +1.1
1,518,425 + b1450 b1450 0.21 +1.6
1,518,495 + b1450 b1450 0.31 +1.3
1,518,498 + b1450 b1450 0.32 +0.3
1,518,506 + b1450 b1450 0.33 -0.6
1,518,746 - b1450 b1450 0.69 +2.1
1,518,756 + b1450 b1450 0.71 -0.0
1,518,756 + b1450 b1450 0.71 +0.5
1,518,767 + b1450 b1450 0.72 +0.3
1,518,775 - b1450 b1450 0.73 +1.1
1,518,779 + b1450 b1450 0.74 +0.2
1,518,844 + b1450 b1450 0.84 +1.9
1,518,844 + b1450 b1450 0.84 +3.3
1,518,871 + b1450 b1450 0.88 +0.6
1,518,872 + b1450 b1450 0.88 +0.7
1,518,954 - +0.2
1,518,974 + -0.7
1,519,053 - +0.5
1,519,084 - -0.1
1,519,089 - +0.1
1,519,228 - yncD b1451 0.11 +0.1
1,519,274 - yncD b1451 0.14 +0.1
1,519,282 - yncD b1451 0.14 +0.0
1,519,282 - yncD b1451 0.14 +0.5
1,519,284 - yncD b1451 0.14 -0.5
1,519,296 + yncD b1451 0.15 +2.1
1,519,313 + yncD b1451 0.16 +0.3
1,519,313 + yncD b1451 0.16 +0.8
1,519,426 + yncD b1451 0.21 +0.4
1,519,485 - yncD b1451 0.24 +0.1
1,519,551 - yncD b1451 0.27 +0.4
1,519,645 + yncD b1451 0.31 +0.0
1,519,653 - yncD b1451 0.32 -0.8
1,519,653 - yncD b1451 0.32 -1.3
1,519,784 - yncD b1451 0.38 +0.1
1,519,828 + yncD b1451 0.40 +0.3

Or see this region's nucleotide sequence