Strain Fitness in Escherichia coli BW25113 around b1261

Experiment: Ying_AminoAcid20 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttrpA and trpB overlap by 1 nucleotidestrpB and trpC are separated by 11 nucleotides b1260: trpA - tryptophan synthase subunit alpha (NCBI), at 1,314,440 to 1,315,246 trpA b1261: trpB - tryptophan synthase subunit beta (NCBI), at 1,315,246 to 1,316,439 trpB b1262: trpC - N-(5-phosphoribosyl)anthranilate isomerase and indole-3-glycerolphosphate synthetase (VIMSS), at 1,316,451 to 1,317,809 trpC Position (kb) 1315 1316 1317Strain fitness (log2 ratio) -1 0 1at 1314.336 kb on + strandat 1314.440 kb on - strandat 1314.440 kb on - strandat 1314.440 kb on - strandat 1314.666 kb on + strand, within trpAat 1314.931 kb on - strand, within trpAat 1315.091 kb on - strand, within trpAat 1315.100 kb on - strand, within trpAat 1315.174 kb on + strandat 1315.218 kb on - strandat 1315.218 kb on - strandat 1315.453 kb on + strand, within trpBat 1315.671 kb on + strand, within trpBat 1315.984 kb on - strand, within trpBat 1316.375 kb on - strandat 1316.375 kb on - strandat 1316.600 kb on + strand, within trpCat 1316.692 kb on + strand, within trpCat 1316.692 kb on + strand, within trpCat 1317.035 kb on - strand, within trpCat 1317.035 kb on - strand, within trpCat 1317.113 kb on - strand, within trpCat 1317.144 kb on - strand, within trpCat 1317.153 kb on + strand, within trpCat 1317.181 kb on + strand, within trpCat 1317.305 kb on - strand, within trpCat 1317.389 kb on + strand, within trpCat 1317.389 kb on + strand, within trpC

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2
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1,314,336 + -0.5
1,314,440 - -0.4
1,314,440 - -0.0
1,314,440 - -0.1
1,314,666 + trpA b1260 0.28 -0.4
1,314,931 - trpA b1260 0.61 -0.2
1,315,091 - trpA b1260 0.81 -0.4
1,315,100 - trpA b1260 0.82 -0.6
1,315,174 + +0.3
1,315,218 - -0.3
1,315,218 - -0.3
1,315,453 + trpB b1261 0.17 +0.8
1,315,671 + trpB b1261 0.36 -0.2
1,315,984 - trpB b1261 0.62 +0.7
1,316,375 - -0.5
1,316,375 - +0.1
1,316,600 + trpC b1262 0.11 +0.4
1,316,692 + trpC b1262 0.18 +0.0
1,316,692 + trpC b1262 0.18 +0.0
1,317,035 - trpC b1262 0.43 -0.8
1,317,035 - trpC b1262 0.43 -1.1
1,317,113 - trpC b1262 0.49 +0.1
1,317,144 - trpC b1262 0.51 +0.1
1,317,153 + trpC b1262 0.52 +0.0
1,317,181 + trpC b1262 0.54 +0.4
1,317,305 - trpC b1262 0.63 -0.8
1,317,389 + trpC b1262 0.69 +0.2
1,317,389 + trpC b1262 0.69 +0.1

Or see this region's nucleotide sequence