Strain Fitness in Escherichia coli BW25113 around b0920

Experiment: Ying_AminoAcid20 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycbJ and ycbC overlap by 24 nucleotidesycbC and smtA are separated by 135 nucleotidessmtA and mukF overlap by 4 nucleotides b0919: ycbJ - hypothetical protein (NCBI), at 970,975 to 971,868 ycbJ b0920: ycbC - conserved inner membrane protein (NCBI), at 971,845 to 972,624 ycbC b0921: smtA - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 972,760 to 973,545 smtA b0922: mukF - condesin subunit F (NCBI), at 973,542 to 974,864 mukF Position (kb) 971 972 973Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 970.868 kb on - strandat 970.868 kb on - strandat 971.000 kb on + strandat 971.061 kb on + strandat 971.071 kb on + strand, within ycbJat 971.221 kb on - strand, within ycbJat 971.291 kb on + strand, within ycbJat 971.291 kb on + strand, within ycbJat 971.300 kb on + strand, within ycbJat 971.300 kb on + strand, within ycbJat 971.348 kb on - strand, within ycbJat 971.348 kb on - strand, within ycbJat 971.350 kb on + strand, within ycbJat 971.382 kb on + strand, within ycbJat 971.382 kb on + strand, within ycbJat 971.426 kb on + strand, within ycbJat 971.434 kb on - strand, within ycbJat 971.436 kb on + strand, within ycbJat 971.442 kb on + strand, within ycbJat 971.442 kb on + strand, within ycbJat 971.447 kb on + strand, within ycbJat 971.447 kb on + strand, within ycbJat 971.447 kb on + strand, within ycbJat 971.706 kb on + strand, within ycbJat 972.023 kb on - strand, within ycbCat 972.065 kb on - strand, within ycbCat 972.215 kb on - strand, within ycbCat 972.313 kb on - strand, within ycbCat 972.351 kb on - strand, within ycbCat 972.354 kb on + strand, within ycbCat 972.354 kb on + strand, within ycbCat 972.535 kb on + strand, within ycbCat 972.548 kb on - strandat 972.819 kb on + strandat 972.852 kb on + strand, within smtAat 972.985 kb on + strand, within smtAat 973.035 kb on - strand, within smtAat 973.035 kb on - strand, within smtAat 973.068 kb on + strand, within smtAat 973.068 kb on + strand, within smtAat 973.113 kb on - strand, within smtAat 973.128 kb on - strand, within smtAat 973.166 kb on + strand, within smtAat 973.169 kb on - strand, within smtAat 973.228 kb on - strand, within smtAat 973.228 kb on - strand, within smtAat 973.307 kb on - strand, within smtAat 973.381 kb on - strand, within smtAat 973.397 kb on - strand, within smtAat 973.416 kb on - strand, within smtAat 973.416 kb on - strand, within smtAat 973.431 kb on + strand, within smtAat 973.507 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2
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970,868 - -0.0
970,868 - -0.4
971,000 + +0.4
971,061 + +1.1
971,071 + ycbJ b0919 0.11 +0.7
971,221 - ycbJ b0919 0.28 +0.5
971,291 + ycbJ b0919 0.35 -0.4
971,291 + ycbJ b0919 0.35 -0.2
971,300 + ycbJ b0919 0.36 -0.7
971,300 + ycbJ b0919 0.36 +0.2
971,348 - ycbJ b0919 0.42 +0.3
971,348 - ycbJ b0919 0.42 +0.7
971,350 + ycbJ b0919 0.42 -0.0
971,382 + ycbJ b0919 0.46 -1.7
971,382 + ycbJ b0919 0.46 +0.4
971,426 + ycbJ b0919 0.50 +0.1
971,434 - ycbJ b0919 0.51 -0.2
971,436 + ycbJ b0919 0.52 -0.3
971,442 + ycbJ b0919 0.52 +0.6
971,442 + ycbJ b0919 0.52 +0.2
971,447 + ycbJ b0919 0.53 +0.4
971,447 + ycbJ b0919 0.53 +0.3
971,447 + ycbJ b0919 0.53 +0.1
971,706 + ycbJ b0919 0.82 +0.1
972,023 - ycbC b0920 0.23 -0.1
972,065 - ycbC b0920 0.28 +0.2
972,215 - ycbC b0920 0.47 +0.6
972,313 - ycbC b0920 0.60 +0.5
972,351 - ycbC b0920 0.65 +0.8
972,354 + ycbC b0920 0.65 +0.6
972,354 + ycbC b0920 0.65 +0.1
972,535 + ycbC b0920 0.88 +1.1
972,548 - +0.4
972,819 + -0.0
972,852 + smtA b0921 0.12 -0.2
972,985 + smtA b0921 0.29 +0.1
973,035 - smtA b0921 0.35 -0.9
973,035 - smtA b0921 0.35 -0.3
973,068 + smtA b0921 0.39 +0.1
973,068 + smtA b0921 0.39 -0.3
973,113 - smtA b0921 0.45 -3.9
973,128 - smtA b0921 0.47 +0.1
973,166 + smtA b0921 0.52 -0.2
973,169 - smtA b0921 0.52 +0.6
973,228 - smtA b0921 0.60 -0.1
973,228 - smtA b0921 0.60 -0.2
973,307 - smtA b0921 0.70 +0.1
973,381 - smtA b0921 0.79 -0.3
973,397 - smtA b0921 0.81 +0.2
973,416 - smtA b0921 0.83 -0.0
973,416 - smtA b0921 0.83 +0.3
973,431 + smtA b0921 0.85 -0.0
973,507 - -0.3

Or see this region's nucleotide sequence