Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgT and yagQ overlap by 22 nucleotides yagQ and yagR are separated by 9 nucleotides yagR and yagS overlap by 4 nucleotides yagS and yagT overlap by 4 nucleotides
b4695: ykgT - no description, at 296,938 to 297,015
ykgT
b0283: yagQ - hypothetical protein (NCBI), at 296,994 to 297,950
yagQ
b0284: yagR - predicted oxidoreductase with molybdenum-binding domain (NCBI), at 297,960 to 300,158
yagR
b0285: yagS - predicted oxidoreductase with FAD-binding domain (NCBI), at 300,155 to 301,111
yagS
b0286: yagT - predicted xanthine dehydrogenase, 2Fe-2S subunit (NCBI), at 301,108 to 301,797
yagT
Position (kb) 297
298
299
300
301 Strain fitness (log2 ratio) -1
0
1 at 296.991 kb on + strand, within ykgT at 296.993 kb on - strand, within ykgT at 296.993 kb on - strand, within ykgT at 297.046 kb on - strand at 297.064 kb on + strand at 297.064 kb on + strand at 297.068 kb on + strand at 297.068 kb on + strand at 297.082 kb on - strand at 297.111 kb on - strand, within yagQ at 297.111 kb on - strand, within yagQ at 297.144 kb on - strand, within yagQ at 297.144 kb on - strand, within yagQ at 297.185 kb on + strand, within yagQ at 297.185 kb on + strand, within yagQ at 297.193 kb on - strand, within yagQ at 297.226 kb on - strand, within yagQ at 297.226 kb on - strand, within yagQ at 297.316 kb on - strand, within yagQ at 297.316 kb on - strand, within yagQ at 297.432 kb on - strand, within yagQ at 297.455 kb on - strand, within yagQ at 297.455 kb on - strand, within yagQ at 297.513 kb on + strand, within yagQ at 297.581 kb on + strand, within yagQ at 297.950 kb on - strand at 298.066 kb on + strand at 298.138 kb on - strand at 298.138 kb on - strand at 298.187 kb on - strand, within yagR at 298.267 kb on - strand, within yagR at 298.267 kb on - strand, within yagR at 298.449 kb on + strand, within yagR at 298.449 kb on + strand, within yagR at 298.549 kb on - strand, within yagR at 298.581 kb on + strand, within yagR at 298.632 kb on + strand, within yagR at 298.728 kb on + strand, within yagR at 298.738 kb on + strand, within yagR at 298.770 kb on + strand, within yagR at 298.903 kb on + strand, within yagR at 298.906 kb on - strand, within yagR at 298.908 kb on + strand, within yagR at 298.908 kb on + strand, within yagR at 298.908 kb on + strand, within yagR at 299.169 kb on + strand, within yagR at 299.206 kb on - strand, within yagR at 299.206 kb on - strand, within yagR at 299.218 kb on + strand, within yagR at 299.218 kb on + strand, within yagR at 299.341 kb on - strand, within yagR at 299.341 kb on - strand, within yagR at 299.413 kb on + strand, within yagR at 299.543 kb on + strand, within yagR at 299.952 kb on + strand at 299.952 kb on + strand at 300.038 kb on + strand at 300.109 kb on + strand at 300.109 kb on + strand at 300.176 kb on + strand at 300.181 kb on + strand at 300.181 kb on + strand at 300.196 kb on - strand at 300.268 kb on - strand, within yagS at 300.268 kb on - strand, within yagS at 300.325 kb on + strand, within yagS at 300.385 kb on + strand, within yagS at 300.483 kb on + strand, within yagS at 300.486 kb on - strand, within yagS at 300.486 kb on - strand, within yagS at 300.544 kb on + strand, within yagS at 300.552 kb on - strand, within yagS at 300.581 kb on + strand, within yagS at 300.719 kb on + strand, within yagS at 300.798 kb on + strand, within yagS at 300.829 kb on - strand, within yagS at 300.887 kb on - strand, within yagS at 300.916 kb on - strand, within yagS at 301.099 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 296,991 + ykgT b4695 0.68 +0.2 296,993 - ykgT b4695 0.71 +0.7 296,993 - ykgT b4695 0.71 +0.8 297,046 - +0.6 297,064 + +0.2 297,064 + +0.2 297,068 + -0.8 297,068 + +0.0 297,082 - +0.1 297,111 - yagQ b0283 0.12 -0.9 297,111 - yagQ b0283 0.12 +0.1 297,144 - yagQ b0283 0.16 -0.3 297,144 - yagQ b0283 0.16 +0.5 297,185 + yagQ b0283 0.20 +0.4 297,185 + yagQ b0283 0.20 -0.1 297,193 - yagQ b0283 0.21 -0.0 297,226 - yagQ b0283 0.24 +1.3 297,226 - yagQ b0283 0.24 +0.3 297,316 - yagQ b0283 0.34 +0.4 297,316 - yagQ b0283 0.34 -0.3 297,432 - yagQ b0283 0.46 +0.3 297,455 - yagQ b0283 0.48 +0.1 297,455 - yagQ b0283 0.48 -0.3 297,513 + yagQ b0283 0.54 -0.4 297,581 + yagQ b0283 0.61 +0.1 297,950 - -0.8 298,066 + -0.4 298,138 - +0.1 298,138 - -1.3 298,187 - yagR b0284 0.10 -0.2 298,267 - yagR b0284 0.14 -0.3 298,267 - yagR b0284 0.14 -0.1 298,449 + yagR b0284 0.22 -0.3 298,449 + yagR b0284 0.22 -0.9 298,549 - yagR b0284 0.27 +0.1 298,581 + yagR b0284 0.28 +0.5 298,632 + yagR b0284 0.31 +0.0 298,728 + yagR b0284 0.35 -0.3 298,738 + yagR b0284 0.35 -0.3 298,770 + yagR b0284 0.37 -0.0 298,903 + yagR b0284 0.43 -0.1 298,906 - yagR b0284 0.43 +0.2 298,908 + yagR b0284 0.43 +1.0 298,908 + yagR b0284 0.43 +0.0 298,908 + yagR b0284 0.43 -0.3 299,169 + yagR b0284 0.55 -0.2 299,206 - yagR b0284 0.57 +0.1 299,206 - yagR b0284 0.57 +0.2 299,218 + yagR b0284 0.57 -0.1 299,218 + yagR b0284 0.57 +0.4 299,341 - yagR b0284 0.63 +0.0 299,341 - yagR b0284 0.63 +0.2 299,413 + yagR b0284 0.66 +0.3 299,543 + yagR b0284 0.72 -0.1 299,952 + +0.6 299,952 + +0.2 300,038 + +0.1 300,109 + -0.6 300,109 + -0.9 300,176 + -1.0 300,181 + -0.5 300,181 + -0.4 300,196 - -0.9 300,268 - yagS b0285 0.12 -0.5 300,268 - yagS b0285 0.12 -0.1 300,325 + yagS b0285 0.18 +1.7 300,385 + yagS b0285 0.24 +0.4 300,483 + yagS b0285 0.34 +0.4 300,486 - yagS b0285 0.35 +0.0 300,486 - yagS b0285 0.35 -0.4 300,544 + yagS b0285 0.41 +0.3 300,552 - yagS b0285 0.41 +0.2 300,581 + yagS b0285 0.45 -0.0 300,719 + yagS b0285 0.59 -0.1 300,798 + yagS b0285 0.67 -0.1 300,829 - yagS b0285 0.70 -0.2 300,887 - yagS b0285 0.76 -0.2 300,916 - yagS b0285 0.80 -0.2 301,099 - +0.3
Or see this region's nucleotide sequence