Experiment: Ying_AminoAcid20 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yadS and btuF are separated by 37 nucleotides btuF and pfs overlap by 8 nucleotides pfs and dgt are separated by 83 nucleotides
b0157: yadS - conserved inner membrane protein (NCBI), at 177,001 to 177,624
yadS
b0158: btuF - vitamin B12-transporter protein BtuF (NCBI), at 177,662 to 178,462
btuF
b0159: pfs - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (NCBI), at 178,455 to 179,153
pfs
b0160: dgt - deoxyguanosinetriphosphate triphosphohydrolase (NCBI), at 179,237 to 180,754
dgt
Position (kb) 178
179
180 Strain fitness (log2 ratio) -1
0
1
2
3 at 177.531 kb on + strand, within yadS at 177.578 kb on + strand at 177.596 kb on - strand at 177.596 kb on - strand at 177.616 kb on + strand at 177.616 kb on + strand at 177.625 kb on + strand at 177.625 kb on + strand at 177.697 kb on + strand at 177.714 kb on - strand at 177.726 kb on + strand at 177.735 kb on - strand at 178.094 kb on - strand, within btuF at 178.094 kb on - strand, within btuF at 178.138 kb on + strand, within btuF at 178.138 kb on - strand, within btuF at 178.150 kb on - strand, within btuF at 178.150 kb on - strand, within btuF at 178.304 kb on - strand, within btuF at 178.304 kb on - strand, within btuF at 178.333 kb on + strand, within btuF at 178.440 kb on + strand at 178.440 kb on + strand at 178.448 kb on - strand at 178.452 kb on + strand at 179.187 kb on + strand at 179.228 kb on + strand at 179.291 kb on + strand at 179.378 kb on + strand at 179.467 kb on + strand, within dgt at 179.470 kb on - strand, within dgt at 179.470 kb on - strand, within dgt at 179.528 kb on + strand, within dgt at 179.528 kb on + strand, within dgt at 179.529 kb on + strand, within dgt at 179.551 kb on + strand, within dgt at 179.572 kb on + strand, within dgt at 179.572 kb on + strand, within dgt at 179.612 kb on + strand, within dgt at 179.680 kb on + strand, within dgt at 179.680 kb on + strand, within dgt at 179.755 kb on + strand, within dgt at 179.755 kb on + strand, within dgt at 179.791 kb on - strand, within dgt at 179.801 kb on - strand, within dgt at 179.802 kb on + strand, within dgt at 179.807 kb on + strand, within dgt at 179.813 kb on + strand, within dgt at 179.813 kb on + strand, within dgt at 179.813 kb on + strand, within dgt at 179.813 kb on - strand, within dgt at 179.821 kb on - strand, within dgt at 179.821 kb on - strand, within dgt at 179.847 kb on - strand, within dgt at 179.847 kb on - strand, within dgt at 179.847 kb on - strand, within dgt at 179.882 kb on + strand, within dgt at 179.882 kb on + strand, within dgt at 179.882 kb on + strand, within dgt at 179.894 kb on + strand, within dgt at 179.902 kb on + strand, within dgt at 179.902 kb on + strand, within dgt at 179.902 kb on + strand, within dgt at 179.902 kb on + strand, within dgt at 179.903 kb on + strand, within dgt at 179.907 kb on + strand, within dgt at 179.910 kb on - strand, within dgt at 179.974 kb on - strand, within dgt at 179.974 kb on - strand, within dgt at 179.976 kb on + strand, within dgt at 179.981 kb on + strand, within dgt at 180.011 kb on + strand, within dgt at 180.011 kb on + strand, within dgt at 180.022 kb on + strand, within dgt at 180.032 kb on - strand, within dgt at 180.044 kb on + strand, within dgt at 180.044 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.046 kb on + strand, within dgt at 180.051 kb on + strand, within dgt at 180.051 kb on + strand, within dgt at 180.052 kb on - strand, within dgt at 180.054 kb on + strand, within dgt at 180.054 kb on + strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.054 kb on - strand, within dgt at 180.059 kb on - strand, within dgt at 180.059 kb on - strand, within dgt at 180.062 kb on + strand, within dgt at 180.063 kb on + strand, within dgt at 180.064 kb on - strand, within dgt at 180.072 kb on - strand, within dgt at 180.072 kb on - strand, within dgt at 180.091 kb on + strand, within dgt at 180.106 kb on + strand, within dgt at 180.114 kb on - strand, within dgt at 180.116 kb on + strand, within dgt at 180.129 kb on + strand, within dgt at 180.134 kb on - strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.139 kb on + strand, within dgt at 180.142 kb on - strand, within dgt
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep A; time point 2 remove 177,531 + yadS b0157 0.85 -0.1 177,578 + +0.7 177,596 - -0.4 177,596 - +0.2 177,616 + -0.2 177,616 + -0.5 177,625 + -0.5 177,625 + +0.5 177,697 + -0.4 177,714 - -0.1 177,726 + +0.2 177,735 - +0.2 178,094 - btuF b0158 0.54 -0.0 178,094 - btuF b0158 0.54 -0.3 178,138 + btuF b0158 0.59 +0.3 178,138 - btuF b0158 0.59 +0.2 178,150 - btuF b0158 0.61 +0.7 178,150 - btuF b0158 0.61 +0.5 178,304 - btuF b0158 0.80 +0.0 178,304 - btuF b0158 0.80 -0.2 178,333 + btuF b0158 0.84 -0.1 178,440 + -0.4 178,440 + -0.5 178,448 - -0.1 178,452 + +0.5 179,187 + +0.1 179,228 + +0.2 179,291 + -0.1 179,378 + +0.6 179,467 + dgt b0160 0.15 -0.1 179,470 - dgt b0160 0.15 +1.2 179,470 - dgt b0160 0.15 +0.5 179,528 + dgt b0160 0.19 +0.1 179,528 + dgt b0160 0.19 -0.4 179,529 + dgt b0160 0.19 +0.6 179,551 + dgt b0160 0.21 +0.2 179,572 + dgt b0160 0.22 +0.6 179,572 + dgt b0160 0.22 +0.8 179,612 + dgt b0160 0.25 +0.4 179,680 + dgt b0160 0.29 +0.3 179,680 + dgt b0160 0.29 +0.7 179,755 + dgt b0160 0.34 +0.2 179,755 + dgt b0160 0.34 -0.7 179,791 - dgt b0160 0.36 +0.2 179,801 - dgt b0160 0.37 +1.0 179,802 + dgt b0160 0.37 +0.6 179,807 + dgt b0160 0.38 -0.5 179,813 + dgt b0160 0.38 +0.4 179,813 + dgt b0160 0.38 +0.3 179,813 + dgt b0160 0.38 +0.1 179,813 - dgt b0160 0.38 +0.2 179,821 - dgt b0160 0.38 +0.5 179,821 - dgt b0160 0.38 +3.0 179,847 - dgt b0160 0.40 +0.6 179,847 - dgt b0160 0.40 -0.1 179,847 - dgt b0160 0.40 +0.5 179,882 + dgt b0160 0.42 +0.6 179,882 + dgt b0160 0.42 +0.6 179,882 + dgt b0160 0.42 +0.1 179,894 + dgt b0160 0.43 +0.5 179,902 + dgt b0160 0.44 +0.2 179,902 + dgt b0160 0.44 +1.2 179,902 + dgt b0160 0.44 +0.9 179,902 + dgt b0160 0.44 +0.3 179,903 + dgt b0160 0.44 +0.6 179,907 + dgt b0160 0.44 +0.2 179,910 - dgt b0160 0.44 -0.7 179,974 - dgt b0160 0.49 +0.2 179,974 - dgt b0160 0.49 +0.7 179,976 + dgt b0160 0.49 -0.1 179,981 + dgt b0160 0.49 +0.3 180,011 + dgt b0160 0.51 +0.8 180,011 + dgt b0160 0.51 -0.0 180,022 + dgt b0160 0.52 -0.0 180,032 - dgt b0160 0.52 -0.3 180,044 + dgt b0160 0.53 -0.4 180,044 + dgt b0160 0.53 +0.1 180,046 + dgt b0160 0.53 -0.9 180,046 + dgt b0160 0.53 -0.5 180,046 + dgt b0160 0.53 +0.6 180,046 + dgt b0160 0.53 +0.3 180,046 + dgt b0160 0.53 +1.0 180,046 + dgt b0160 0.53 +0.1 180,046 + dgt b0160 0.53 +1.3 180,046 + dgt b0160 0.53 +0.8 180,051 + dgt b0160 0.54 +0.2 180,051 + dgt b0160 0.54 +1.4 180,052 - dgt b0160 0.54 +0.2 180,054 + dgt b0160 0.54 -0.2 180,054 + dgt b0160 0.54 +0.5 180,054 - dgt b0160 0.54 +1.3 180,054 - dgt b0160 0.54 +0.4 180,054 - dgt b0160 0.54 +0.8 180,054 - dgt b0160 0.54 -0.2 180,054 - dgt b0160 0.54 -0.2 180,054 - dgt b0160 0.54 +0.8 180,059 - dgt b0160 0.54 +0.1 180,059 - dgt b0160 0.54 +0.3 180,062 + dgt b0160 0.54 -0.1 180,063 + dgt b0160 0.54 +0.3 180,064 - dgt b0160 0.54 +0.5 180,072 - dgt b0160 0.55 +0.2 180,072 - dgt b0160 0.55 +1.8 180,091 + dgt b0160 0.56 -0.4 180,106 + dgt b0160 0.57 -0.1 180,114 - dgt b0160 0.58 +0.4 180,116 + dgt b0160 0.58 +0.2 180,129 + dgt b0160 0.59 +0.5 180,134 - dgt b0160 0.59 +1.0 180,139 + dgt b0160 0.59 -0.3 180,139 + dgt b0160 0.59 -0.2 180,139 + dgt b0160 0.59 +0.5 180,139 + dgt b0160 0.59 -0.1 180,139 + dgt b0160 0.59 +0.5 180,139 + dgt b0160 0.59 +0.1 180,139 + dgt b0160 0.59 +0.3 180,142 - dgt b0160 0.60 +0.6
Or see this region's nucleotide sequence