Experiment: Ying_all64 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgaT and ulaB are separated by 15 nucleotides ulaB and ulaC are separated by 9 nucleotides ulaC and ulaD are separated by 13 nucleotides ulaD and ulaE are separated by 9 nucleotides ulaE and ulaF overlap by 1 nucleotides
b4193: sgaT - orf, hypothetical protein (VIMSS), at 4,418,003 to 4,419,400
sgaT
b4194: ulaB - L-ascorbate-specific enzyme IIB component of PTS (NCBI), at 4,419,416 to 4,419,721
ulaB
b4195: ulaC - L-ascorbate-specific enzyme IIA component of PTS (NCBI), at 4,419,731 to 4,420,195
ulaC
b4196: ulaD - 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI), at 4,420,209 to 4,420,859
ulaD
b4197: ulaE - L-xylulose 5-phosphate 3-epimerase (NCBI), at 4,420,869 to 4,421,723
ulaE
b4198: ulaF - L-ribulose 5-phosphate 4-epimerase (NCBI), at 4,421,723 to 4,422,409
ulaF
Position (kb) 4420
4421 Strain fitness (log2 ratio) -1
0
1 at 4419.245 kb on + strand, within sgaT at 4419.291 kb on + strand at 4419.357 kb on + strand at 4419.357 kb on + strand at 4419.431 kb on - strand at 4419.457 kb on - strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.630 kb on + strand, within ulaB at 4419.869 kb on + strand, within ulaC at 4419.869 kb on + strand, within ulaC at 4419.924 kb on - strand, within ulaC at 4419.929 kb on - strand, within ulaC at 4419.947 kb on - strand, within ulaC at 4420.137 kb on + strand, within ulaC at 4420.164 kb on + strand at 4420.164 kb on + strand at 4420.206 kb on + strand at 4420.355 kb on - strand, within ulaD at 4420.363 kb on + strand, within ulaD at 4420.386 kb on - strand, within ulaD at 4420.479 kb on + strand, within ulaD at 4420.533 kb on + strand, within ulaD at 4420.641 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD at 4420.769 kb on + strand, within ulaD at 4420.848 kb on - strand at 4420.898 kb on + strand at 4420.898 kb on + strand at 4421.038 kb on + strand, within ulaE at 4421.046 kb on - strand, within ulaE at 4421.046 kb on - strand, within ulaE at 4421.148 kb on - strand, within ulaE at 4421.156 kb on + strand, within ulaE at 4421.210 kb on - strand, within ulaE at 4421.210 kb on - strand, within ulaE at 4421.354 kb on - strand, within ulaE at 4421.354 kb on - strand, within ulaE at 4421.387 kb on - strand, within ulaE at 4421.387 kb on - strand, within ulaE at 4421.449 kb on + strand, within ulaE at 4421.459 kb on - strand, within ulaE at 4421.471 kb on - strand, within ulaE at 4421.496 kb on + strand at 4421.764 kb on - strand at 4421.811 kb on - strand, within ulaF at 4421.811 kb on - strand, within ulaF
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2 remove 4,419,245 + sgaT b4193 0.89 -0.3 4,419,291 + -0.1 4,419,357 + -0.6 4,419,357 + +0.5 4,419,431 - +0.0 4,419,457 - ulaB b4194 0.13 +0.3 4,419,584 + ulaB b4194 0.55 -0.2 4,419,584 + ulaB b4194 0.55 -0.2 4,419,584 + ulaB b4194 0.55 -0.2 4,419,630 + ulaB b4194 0.70 -0.1 4,419,869 + ulaC b4195 0.30 +0.2 4,419,869 + ulaC b4195 0.30 -0.4 4,419,924 - ulaC b4195 0.42 -0.4 4,419,929 - ulaC b4195 0.43 -0.0 4,419,947 - ulaC b4195 0.46 +0.4 4,420,137 + ulaC b4195 0.87 +0.9 4,420,164 + -0.2 4,420,164 + -0.2 4,420,206 + +0.2 4,420,355 - ulaD b4196 0.22 +0.9 4,420,363 + ulaD b4196 0.24 -0.5 4,420,386 - ulaD b4196 0.27 +0.8 4,420,479 + ulaD b4196 0.41 +0.1 4,420,533 + ulaD b4196 0.50 -0.3 4,420,641 + ulaD b4196 0.66 +1.1 4,420,719 + ulaD b4196 0.78 +0.7 4,420,719 + ulaD b4196 0.78 +0.1 4,420,769 + ulaD b4196 0.86 +0.2 4,420,848 - +0.5 4,420,898 + -0.1 4,420,898 + +0.6 4,421,038 + ulaE b4197 0.20 +0.1 4,421,046 - ulaE b4197 0.21 +0.0 4,421,046 - ulaE b4197 0.21 -0.3 4,421,148 - ulaE b4197 0.33 +0.2 4,421,156 + ulaE b4197 0.34 -0.1 4,421,210 - ulaE b4197 0.40 +0.3 4,421,210 - ulaE b4197 0.40 +0.2 4,421,354 - ulaE b4197 0.57 +0.1 4,421,354 - ulaE b4197 0.57 -0.1 4,421,387 - ulaE b4197 0.61 +0.0 4,421,387 - ulaE b4197 0.61 +0.2 4,421,449 + ulaE b4197 0.68 -0.3 4,421,459 - ulaE b4197 0.69 -0.1 4,421,471 - ulaE b4197 0.70 +0.1 4,421,496 + -0.3 4,421,764 - -0.2 4,421,811 - ulaF b4198 0.13 +0.4 4,421,811 - ulaF b4198 0.13 -0.9
Or see this region's nucleotide sequence