Strain Fitness in Escherichia coli BW25113 around b3083

Experiment: Ying_all64 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfadH and ygjM are separated by 44 nucleotidesygjM and ygjN overlap by 4 nucleotidesygjN and ygjO are separated by 283 nucleotides b3081: fadH - 2,4-dienoyl-CoA reductase, NADH and FMN-linked (NCBI), at 3,229,687 to 3,231,705 fadH b3082: ygjM - predicted DNA-binding transcriptional regulator (NCBI), at 3,231,750 to 3,232,166 ygjM b3083: ygjN - hypothetical protein (NCBI), at 3,232,163 to 3,232,477 ygjN b3084: ygjO - putative enzyme (VIMSS), at 3,232,761 to 3,233,897 ygjO Position (kb) 3232 3233Strain fitness (log2 ratio) -1 0 1at 3231.163 kb on - strand, within fadHat 3231.199 kb on - strand, within fadHat 3231.267 kb on + strand, within fadHat 3231.267 kb on + strand, within fadHat 3231.356 kb on + strand, within fadHat 3231.399 kb on - strand, within fadHat 3231.427 kb on - strand, within fadHat 3231.428 kb on + strand, within fadHat 3231.428 kb on + strand, within fadHat 3231.428 kb on + strand, within fadHat 3231.428 kb on + strand, within fadHat 3231.504 kb on - strandat 3231.530 kb on + strandat 3231.530 kb on + strandat 3231.547 kb on + strandat 3231.552 kb on + strandat 3231.643 kb on + strandat 3231.673 kb on + strandat 3231.812 kb on - strand, within ygjMat 3231.855 kb on - strand, within ygjMat 3231.856 kb on - strand, within ygjMat 3231.892 kb on - strand, within ygjMat 3231.892 kb on - strand, within ygjMat 3232.179 kb on + strandat 3232.179 kb on + strandat 3232.181 kb on + strandat 3232.186 kb on + strandat 3232.189 kb on - strandat 3232.233 kb on + strand, within ygjNat 3232.267 kb on + strand, within ygjNat 3232.272 kb on - strand, within ygjNat 3232.302 kb on + strand, within ygjNat 3232.394 kb on + strand, within ygjNat 3232.416 kb on + strand, within ygjNat 3232.465 kb on - strandat 3232.465 kb on - strandat 3232.465 kb on - strandat 3232.471 kb on + strandat 3232.603 kb on + strandat 3232.603 kb on + strandat 3232.704 kb on - strandat 3232.704 kb on - strandat 3232.783 kb on + strandat 3232.783 kb on + strandat 3232.815 kb on + strandat 3232.830 kb on - strandat 3232.862 kb on + strandat 3232.862 kb on + strandat 3232.924 kb on - strand, within ygjOat 3232.967 kb on - strand, within ygjOat 3232.979 kb on - strand, within ygjOat 3232.979 kb on - strand, within ygjOat 3232.999 kb on - strand, within ygjOat 3232.999 kb on - strand, within ygjOat 3233.263 kb on - strand, within ygjOat 3233.263 kb on - strand, within ygjOat 3233.289 kb on + strand, within ygjOat 3233.297 kb on - strand, within ygjOat 3233.297 kb on - strand, within ygjOat 3233.297 kb on - strand, within ygjOat 3233.352 kb on - strand, within ygjOat 3233.352 kb on - strand, within ygjOat 3233.358 kb on - strand, within ygjOat 3233.358 kb on - strand, within ygjOat 3233.402 kb on + strand, within ygjO

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2
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3,231,163 - fadH b3081 0.73 +0.0
3,231,199 - fadH b3081 0.75 +0.1
3,231,267 + fadH b3081 0.78 +0.2
3,231,267 + fadH b3081 0.78 +0.7
3,231,356 + fadH b3081 0.83 +0.2
3,231,399 - fadH b3081 0.85 -0.4
3,231,427 - fadH b3081 0.86 +0.4
3,231,428 + fadH b3081 0.86 -0.8
3,231,428 + fadH b3081 0.86 +0.6
3,231,428 + fadH b3081 0.86 +0.2
3,231,428 + fadH b3081 0.86 -0.2
3,231,504 - +0.6
3,231,530 + -1.0
3,231,530 + -0.4
3,231,547 + -0.3
3,231,552 + +0.7
3,231,643 + +0.0
3,231,673 + +0.4
3,231,812 - ygjM b3082 0.15 -0.7
3,231,855 - ygjM b3082 0.25 -0.3
3,231,856 - ygjM b3082 0.25 -0.3
3,231,892 - ygjM b3082 0.34 +1.3
3,231,892 - ygjM b3082 0.34 -0.3
3,232,179 + -0.2
3,232,179 + -0.2
3,232,181 + +0.4
3,232,186 + +0.6
3,232,189 - +0.5
3,232,233 + ygjN b3083 0.22 -0.1
3,232,267 + ygjN b3083 0.33 +0.2
3,232,272 - ygjN b3083 0.35 +0.9
3,232,302 + ygjN b3083 0.44 +0.3
3,232,394 + ygjN b3083 0.73 +0.2
3,232,416 + ygjN b3083 0.80 +0.1
3,232,465 - -0.6
3,232,465 - -0.5
3,232,465 - +0.2
3,232,471 + +1.4
3,232,603 + +0.6
3,232,603 + +0.0
3,232,704 - -1.1
3,232,704 - +0.7
3,232,783 + +0.2
3,232,783 + +0.2
3,232,815 + -0.2
3,232,830 - +0.7
3,232,862 + -0.7
3,232,862 + -0.1
3,232,924 - ygjO b3084 0.14 -0.8
3,232,967 - ygjO b3084 0.18 +0.2
3,232,979 - ygjO b3084 0.19 +0.1
3,232,979 - ygjO b3084 0.19 +0.3
3,232,999 - ygjO b3084 0.21 +0.6
3,232,999 - ygjO b3084 0.21 -0.3
3,233,263 - ygjO b3084 0.44 -0.5
3,233,263 - ygjO b3084 0.44 -0.0
3,233,289 + ygjO b3084 0.46 +0.1
3,233,297 - ygjO b3084 0.47 +0.7
3,233,297 - ygjO b3084 0.47 +0.5
3,233,297 - ygjO b3084 0.47 +0.5
3,233,352 - ygjO b3084 0.52 +0.4
3,233,352 - ygjO b3084 0.52 +0.5
3,233,358 - ygjO b3084 0.53 -0.2
3,233,358 - ygjO b3084 0.53 +0.4
3,233,402 + ygjO b3084 0.56 +0.4

Or see this region's nucleotide sequence