Strain Fitness in Escherichia coli BW25113 around b1064

Experiment: Ying_all64 rep C; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyrC and yceB are separated by 105 nucleotidesyceB and grxB are separated by 133 nucleotidesgrxB and yceL are separated by 63 nucleotides b1062: pyrC - dihydroorotase (NCBI), at 1,120,784 to 1,121,830 pyrC b1063: yceB - predicted lipoprotein (NCBI), at 1,121,936 to 1,122,496 yceB b1064: grxB - glutaredoxin 2 (Grx2) (NCBI), at 1,122,630 to 1,123,277 grxB b1065: yceL - orf, hypothetical protein (VIMSS), at 1,123,341 to 1,124,549 yceL Position (kb) 1122 1123 1124Strain fitness (log2 ratio) -1 0 1at 1121.636 kb on + strand, within pyrCat 1121.641 kb on + strand, within pyrCat 1121.668 kb on + strand, within pyrCat 1121.668 kb on + strand, within pyrCat 1121.689 kb on + strand, within pyrCat 1121.689 kb on + strand, within pyrCat 1121.694 kb on + strand, within pyrCat 1121.793 kb on + strandat 1121.793 kb on + strandat 1122.106 kb on - strand, within yceBat 1122.106 kb on - strand, within yceBat 1122.256 kb on - strand, within yceBat 1122.315 kb on + strand, within yceBat 1122.325 kb on + strand, within yceBat 1122.356 kb on + strand, within yceBat 1122.356 kb on + strand, within yceBat 1122.501 kb on + strandat 1122.533 kb on + strandat 1122.546 kb on - strandat 1122.549 kb on - strandat 1122.556 kb on + strandat 1122.689 kb on + strandat 1122.829 kb on - strand, within grxBat 1122.942 kb on - strand, within grxBat 1123.018 kb on - strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.099 kb on + strand, within grxBat 1123.195 kb on - strand, within grxBat 1123.274 kb on + strandat 1123.322 kb on + strandat 1123.322 kb on + strandat 1123.329 kb on - strandat 1123.443 kb on + strandat 1123.891 kb on - strand, within yceLat 1123.895 kb on - strand, within yceLat 1123.978 kb on - strand, within yceLat 1124.118 kb on + strand, within yceLat 1124.126 kb on - strand, within yceLat 1124.164 kb on - strand, within yceLat 1124.215 kb on - strand, within yceL

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2
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1,121,636 + pyrC b1062 0.81 +0.4
1,121,641 + pyrC b1062 0.82 -0.0
1,121,668 + pyrC b1062 0.84 -0.4
1,121,668 + pyrC b1062 0.84 +0.1
1,121,689 + pyrC b1062 0.86 +1.0
1,121,689 + pyrC b1062 0.86 -0.0
1,121,694 + pyrC b1062 0.87 -0.4
1,121,793 + +0.6
1,121,793 + +0.6
1,122,106 - yceB b1063 0.30 +0.8
1,122,106 - yceB b1063 0.30 +0.4
1,122,256 - yceB b1063 0.57 -0.2
1,122,315 + yceB b1063 0.68 -0.1
1,122,325 + yceB b1063 0.69 +0.5
1,122,356 + yceB b1063 0.75 +0.8
1,122,356 + yceB b1063 0.75 -0.0
1,122,501 + +0.2
1,122,533 + -0.3
1,122,546 - -0.0
1,122,549 - -0.1
1,122,556 + -0.1
1,122,689 + +0.1
1,122,829 - grxB b1064 0.31 +0.1
1,122,942 - grxB b1064 0.48 +0.4
1,123,018 - grxB b1064 0.60 +0.2
1,123,099 + grxB b1064 0.72 +0.6
1,123,099 + grxB b1064 0.72 +0.5
1,123,099 + grxB b1064 0.72 +0.4
1,123,195 - grxB b1064 0.87 +0.4
1,123,274 + +0.3
1,123,322 + -0.8
1,123,322 + -0.3
1,123,329 - +0.5
1,123,443 + -0.5
1,123,891 - yceL b1065 0.45 +0.5
1,123,895 - yceL b1065 0.46 -0.7
1,123,978 - yceL b1065 0.53 +0.0
1,124,118 + yceL b1065 0.64 +0.4
1,124,126 - yceL b1065 0.65 -0.3
1,124,164 - yceL b1065 0.68 +0.0
1,124,215 - yceL b1065 0.72 +0.0

Or see this region's nucleotide sequence