Experiment: Ying_all64 rep C; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt citA and citB overlap by 32 nucleotides citB and dcuC are separated by 40 nucleotides dcuC and crcA are separated by 588 nucleotides
b0619: citA - sensory histidine kinase in two-component regulatory system with citB (NCBI), at 651,458 to 653,116
citA
b0620: citB - DNA-binding response regulator in two-component regulatory system with citA (NCBI), at 653,085 to 653,765
citB
b0621: dcuC - anaerobic C4-dicarboxylate transport (NCBI), at 653,806 to 655,191
dcuC
b0622: crcA - palmitoyl transferase for Lipid A (NCBI), at 655,780 to 656,340
crcA
Position (kb) 653
654
655
656 Strain fitness (log2 ratio) -1
0
1
2 at 652.880 kb on + strand, within citA at 652.885 kb on + strand, within citA at 652.893 kb on - strand, within citA at 652.966 kb on - strand at 652.968 kb on + strand at 652.981 kb on - strand at 653.038 kb on + strand at 653.039 kb on - strand at 653.081 kb on + strand at 653.081 kb on + strand at 653.081 kb on + strand at 653.094 kb on + strand at 653.094 kb on + strand at 653.094 kb on + strand at 653.094 kb on + strand at 653.183 kb on - strand, within citB at 653.183 kb on - strand, within citB at 653.213 kb on + strand, within citB at 653.213 kb on + strand, within citB at 653.236 kb on - strand, within citB at 653.238 kb on + strand, within citB at 653.239 kb on + strand, within citB at 653.285 kb on - strand, within citB at 653.328 kb on + strand, within citB at 653.330 kb on + strand, within citB at 653.336 kb on - strand, within citB at 653.350 kb on + strand, within citB at 653.378 kb on - strand, within citB at 653.378 kb on - strand, within citB at 653.381 kb on - strand, within citB at 653.385 kb on + strand, within citB at 653.510 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.534 kb on + strand, within citB at 653.542 kb on - strand, within citB at 653.542 kb on - strand, within citB at 653.557 kb on - strand, within citB at 653.635 kb on + strand, within citB at 653.635 kb on + strand, within citB at 653.646 kb on + strand, within citB at 653.646 kb on + strand, within citB at 653.647 kb on - strand, within citB at 653.659 kb on + strand, within citB at 653.659 kb on + strand, within citB at 653.747 kb on - strand at 653.747 kb on - strand at 653.759 kb on - strand at 653.759 kb on - strand at 653.831 kb on + strand at 653.858 kb on - strand at 653.886 kb on - strand at 653.886 kb on - strand at 653.967 kb on + strand, within dcuC at 654.163 kb on + strand, within dcuC at 654.163 kb on + strand, within dcuC at 654.207 kb on + strand, within dcuC at 654.219 kb on + strand, within dcuC at 654.285 kb on - strand, within dcuC at 654.337 kb on + strand, within dcuC at 654.345 kb on - strand, within dcuC at 654.355 kb on + strand, within dcuC at 654.427 kb on + strand, within dcuC at 654.518 kb on + strand, within dcuC at 654.653 kb on - strand, within dcuC at 654.656 kb on + strand, within dcuC at 654.850 kb on + strand, within dcuC at 654.850 kb on + strand, within dcuC at 654.896 kb on - strand, within dcuC at 654.896 kb on - strand, within dcuC at 654.897 kb on - strand, within dcuC at 654.936 kb on + strand, within dcuC at 655.012 kb on + strand, within dcuC at 655.027 kb on + strand, within dcuC at 655.030 kb on - strand, within dcuC at 655.030 kb on - strand, within dcuC at 655.030 kb on - strand, within dcuC at 655.064 kb on - strand at 655.067 kb on + strand at 655.077 kb on - strand at 655.095 kb on + strand at 655.095 kb on + strand at 655.114 kb on - strand at 655.122 kb on - strand at 655.123 kb on - strand at 655.127 kb on + strand at 655.138 kb on + strand at 655.138 kb on + strand at 655.145 kb on + strand at 655.147 kb on + strand at 655.147 kb on + strand at 655.147 kb on + strand at 655.151 kb on - strand at 655.152 kb on - strand at 655.155 kb on + strand at 655.155 kb on + strand at 655.155 kb on - strand at 655.155 kb on - strand at 655.166 kb on + strand at 655.169 kb on - strand at 655.204 kb on + strand at 655.329 kb on + strand at 655.370 kb on - strand at 655.436 kb on - strand at 655.472 kb on + strand at 655.624 kb on + strand at 655.716 kb on - strand at 655.727 kb on - strand at 655.861 kb on + strand, within crcA at 655.861 kb on + strand, within crcA at 655.866 kb on + strand, within crcA at 655.866 kb on + strand, within crcA at 655.869 kb on - strand, within crcA at 655.874 kb on - strand, within crcA at 655.907 kb on - strand, within crcA at 655.919 kb on - strand, within crcA at 655.919 kb on - strand, within crcA at 655.937 kb on - strand, within crcA at 655.937 kb on - strand, within crcA at 655.946 kb on + strand, within crcA at 655.947 kb on - strand, within crcA at 655.947 kb on - strand, within crcA at 655.949 kb on + strand, within crcA at 655.957 kb on - strand, within crcA at 655.961 kb on - strand, within crcA at 655.996 kb on - strand, within crcA at 655.998 kb on - strand, within crcA at 655.998 kb on - strand, within crcA at 655.999 kb on - strand, within crcA at 655.999 kb on - strand, within crcA at 656.001 kb on + strand, within crcA at 656.009 kb on + strand, within crcA at 656.009 kb on - strand, within crcA at 656.009 kb on - strand, within crcA at 656.009 kb on - strand, within crcA at 656.058 kb on + strand, within crcA at 656.118 kb on - strand, within crcA at 656.118 kb on - strand, within crcA at 656.129 kb on - strand, within crcA at 656.191 kb on - strand, within crcA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep C; time point 2 remove 652,880 + citA b0619 0.86 -0.3 652,885 + citA b0619 0.86 -0.8 652,893 - citA b0619 0.86 +0.5 652,966 - +0.2 652,968 + +0.5 652,981 - +0.5 653,038 + -0.7 653,039 - -1.2 653,081 + +0.1 653,081 + +0.2 653,081 + +0.1 653,094 + -0.3 653,094 + -0.2 653,094 + -1.3 653,094 + +0.3 653,183 - citB b0620 0.14 -0.2 653,183 - citB b0620 0.14 -0.0 653,213 + citB b0620 0.19 -0.1 653,213 + citB b0620 0.19 -1.1 653,236 - citB b0620 0.22 +0.1 653,238 + citB b0620 0.22 -0.5 653,239 + citB b0620 0.23 +0.2 653,285 - citB b0620 0.29 -0.2 653,328 + citB b0620 0.36 +0.3 653,330 + citB b0620 0.36 +0.5 653,336 - citB b0620 0.37 -0.2 653,350 + citB b0620 0.39 -0.6 653,378 - citB b0620 0.43 -0.4 653,378 - citB b0620 0.43 +0.4 653,381 - citB b0620 0.43 -0.6 653,385 + citB b0620 0.44 +0.6 653,510 + citB b0620 0.62 +0.7 653,534 + citB b0620 0.66 +0.3 653,534 + citB b0620 0.66 +0.6 653,534 + citB b0620 0.66 +0.7 653,534 + citB b0620 0.66 +1.0 653,534 + citB b0620 0.66 -0.2 653,542 - citB b0620 0.67 +0.2 653,542 - citB b0620 0.67 -0.0 653,557 - citB b0620 0.69 +0.1 653,635 + citB b0620 0.81 +0.8 653,635 + citB b0620 0.81 -0.5 653,646 + citB b0620 0.82 -0.4 653,646 + citB b0620 0.82 -0.0 653,647 - citB b0620 0.83 +0.1 653,659 + citB b0620 0.84 +0.2 653,659 + citB b0620 0.84 -1.0 653,747 - -0.3 653,747 - -0.4 653,759 - -0.1 653,759 - -0.1 653,831 + +0.3 653,858 - +0.4 653,886 - -0.4 653,886 - +0.8 653,967 + dcuC b0621 0.12 +0.2 654,163 + dcuC b0621 0.26 +1.3 654,163 + dcuC b0621 0.26 +0.3 654,207 + dcuC b0621 0.29 +1.0 654,219 + dcuC b0621 0.30 +0.4 654,285 - dcuC b0621 0.35 +0.6 654,337 + dcuC b0621 0.38 +0.4 654,345 - dcuC b0621 0.39 +0.1 654,355 + dcuC b0621 0.40 -0.0 654,427 + dcuC b0621 0.45 +1.2 654,518 + dcuC b0621 0.51 +0.9 654,653 - dcuC b0621 0.61 -0.2 654,656 + dcuC b0621 0.61 +0.4 654,850 + dcuC b0621 0.75 +0.4 654,850 + dcuC b0621 0.75 -0.0 654,896 - dcuC b0621 0.79 +0.3 654,896 - dcuC b0621 0.79 -0.3 654,897 - dcuC b0621 0.79 -0.4 654,936 + dcuC b0621 0.82 +0.3 655,012 + dcuC b0621 0.87 +0.3 655,027 + dcuC b0621 0.88 +0.1 655,030 - dcuC b0621 0.88 +0.2 655,030 - dcuC b0621 0.88 +0.4 655,030 - dcuC b0621 0.88 -0.2 655,064 - +0.3 655,067 + +0.5 655,077 - +2.6 655,095 + +1.3 655,095 + -0.9 655,114 - +0.5 655,122 - +0.6 655,123 - +1.0 655,127 + -0.4 655,138 + +0.2 655,138 + +0.1 655,145 + -1.5 655,147 + +0.4 655,147 + +1.8 655,147 + +0.3 655,151 - +0.8 655,152 - -1.0 655,155 + +1.3 655,155 + +0.7 655,155 - -0.7 655,155 - +2.6 655,166 + +0.4 655,169 - +2.6 655,204 + -0.3 655,329 + +2.6 655,370 - +2.6 655,436 - +2.6 655,472 + +2.6 655,624 + +0.6 655,716 - +2.6 655,727 - +2.6 655,861 + crcA b0622 0.14 +0.6 655,861 + crcA b0622 0.14 +1.0 655,866 + crcA b0622 0.15 +1.6 655,866 + crcA b0622 0.15 +1.6 655,869 - crcA b0622 0.16 +1.4 655,874 - crcA b0622 0.17 -0.2 655,907 - crcA b0622 0.23 +1.2 655,919 - crcA b0622 0.25 +0.3 655,919 - crcA b0622 0.25 +0.7 655,937 - crcA b0622 0.28 +1.1 655,937 - crcA b0622 0.28 +0.6 655,946 + crcA b0622 0.30 -0.1 655,947 - crcA b0622 0.30 -0.1 655,947 - crcA b0622 0.30 +0.3 655,949 + crcA b0622 0.30 +0.3 655,957 - crcA b0622 0.32 +0.6 655,961 - crcA b0622 0.32 -0.3 655,996 - crcA b0622 0.39 -0.3 655,998 - crcA b0622 0.39 +0.4 655,998 - crcA b0622 0.39 -0.8 655,999 - crcA b0622 0.39 +0.9 655,999 - crcA b0622 0.39 +0.1 656,001 + crcA b0622 0.39 +0.3 656,009 + crcA b0622 0.41 +0.3 656,009 - crcA b0622 0.41 +0.6 656,009 - crcA b0622 0.41 -0.2 656,009 - crcA b0622 0.41 +0.4 656,058 + crcA b0622 0.50 +0.4 656,118 - crcA b0622 0.60 -0.0 656,118 - crcA b0622 0.60 -0.2 656,129 - crcA b0622 0.62 +0.4 656,191 - crcA b0622 0.73 +0.3
Or see this region's nucleotide sequence