Strain Fitness in Escherichia coli BW25113 around b1879

Experiment: Ying_all64 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyecT and flhE are separated by 119 nucleotidesflhE and flhA overlap by 1 nucleotidesflhA and flhB overlap by 8 nucleotides b1877: yecT - orf, hypothetical protein (VIMSS), at 1,959,996 to 1,960,484 yecT b1878: flhE - hypothetical protein (NCBI), at 1,960,604 to 1,960,996 flhE b1879: flhA - flagellar biosynthesis protein A (NCBI), at 1,960,996 to 1,963,074 flhA b1880: flhB - flagellar biosynthesis protein B (NCBI), at 1,963,067 to 1,964,215 flhB Position (kb) 1960 1961 1962 1963 1964Strain fitness (log2 ratio) -2 -1 0 1 2at 1960.031 kb on + strandat 1960.035 kb on + strandat 1960.035 kb on + strandat 1960.040 kb on + strandat 1960.040 kb on + strandat 1960.065 kb on + strand, within yecTat 1960.067 kb on - strand, within yecTat 1960.079 kb on - strand, within yecTat 1960.079 kb on - strand, within yecTat 1960.120 kb on + strand, within yecTat 1960.129 kb on + strand, within yecTat 1960.144 kb on + strand, within yecTat 1960.274 kb on - strand, within yecTat 1960.274 kb on - strand, within yecTat 1960.357 kb on + strand, within yecTat 1960.358 kb on + strand, within yecTat 1960.372 kb on - strand, within yecTat 1960.390 kb on + strand, within yecTat 1960.390 kb on + strand, within yecTat 1960.391 kb on + strand, within yecTat 1960.431 kb on + strand, within yecTat 1960.431 kb on + strand, within yecTat 1960.439 kb on + strandat 1960.511 kb on - strandat 1960.511 kb on - strandat 1960.560 kb on - strandat 1960.646 kb on - strand, within flhEat 1960.680 kb on - strand, within flhEat 1960.723 kb on - strand, within flhEat 1960.723 kb on - strand, within flhEat 1960.813 kb on + strand, within flhEat 1960.844 kb on - strand, within flhEat 1960.846 kb on - strand, within flhEat 1960.846 kb on - strand, within flhEat 1960.882 kb on + strand, within flhEat 1960.882 kb on + strand, within flhEat 1960.988 kb on - strandat 1961.060 kb on - strandat 1961.060 kb on - strandat 1961.071 kb on - strandat 1961.144 kb on + strandat 1961.162 kb on - strandat 1961.264 kb on + strand, within flhAat 1961.329 kb on - strand, within flhAat 1961.415 kb on - strand, within flhAat 1961.422 kb on + strand, within flhAat 1961.456 kb on + strand, within flhAat 1961.513 kb on + strand, within flhAat 1961.576 kb on - strand, within flhAat 1961.576 kb on - strand, within flhAat 1961.623 kb on + strand, within flhAat 1961.644 kb on - strand, within flhAat 1961.967 kb on - strand, within flhAat 1962.000 kb on + strand, within flhAat 1962.040 kb on - strand, within flhAat 1962.040 kb on - strand, within flhAat 1962.040 kb on - strand, within flhAat 1962.154 kb on + strand, within flhAat 1962.257 kb on + strand, within flhAat 1962.265 kb on - strand, within flhAat 1962.265 kb on - strand, within flhAat 1962.513 kb on + strand, within flhAat 1962.513 kb on + strand, within flhAat 1962.958 kb on - strandat 1963.140 kb on - strandat 1963.140 kb on - strandat 1963.206 kb on + strand, within flhBat 1963.214 kb on - strand, within flhBat 1963.214 kb on - strand, within flhBat 1963.245 kb on + strand, within flhBat 1963.349 kb on - strand, within flhBat 1963.501 kb on - strand, within flhBat 1963.599 kb on + strand, within flhBat 1963.636 kb on - strand, within flhBat 1963.647 kb on - strand, within flhBat 1963.647 kb on - strand, within flhBat 1963.705 kb on - strand, within flhBat 1963.946 kb on + strand, within flhBat 1964.064 kb on - strand, within flhB

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2
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1,960,031 + +2.3
1,960,035 + +0.7
1,960,035 + +1.7
1,960,040 + +1.0
1,960,040 + +1.3
1,960,065 + yecT b1877 0.14 +2.3
1,960,067 - yecT b1877 0.15 -0.6
1,960,079 - yecT b1877 0.17 +0.7
1,960,079 - yecT b1877 0.17 -0.0
1,960,120 + yecT b1877 0.25 +1.7
1,960,129 + yecT b1877 0.27 +2.3
1,960,144 + yecT b1877 0.30 -0.7
1,960,274 - yecT b1877 0.57 -0.2
1,960,274 - yecT b1877 0.57 -0.7
1,960,357 + yecT b1877 0.74 -0.7
1,960,358 + yecT b1877 0.74 +2.3
1,960,372 - yecT b1877 0.77 -1.4
1,960,390 + yecT b1877 0.81 -0.3
1,960,390 + yecT b1877 0.81 -0.0
1,960,391 + yecT b1877 0.81 -0.5
1,960,431 + yecT b1877 0.89 +2.3
1,960,431 + yecT b1877 0.89 +0.7
1,960,439 + -1.1
1,960,511 - +0.8
1,960,511 - +0.1
1,960,560 - +0.1
1,960,646 - flhE b1878 0.11 -0.4
1,960,680 - flhE b1878 0.19 -0.2
1,960,723 - flhE b1878 0.30 +0.6
1,960,723 - flhE b1878 0.30 -0.3
1,960,813 + flhE b1878 0.53 +0.9
1,960,844 - flhE b1878 0.61 -0.9
1,960,846 - flhE b1878 0.62 -0.1
1,960,846 - flhE b1878 0.62 -2.4
1,960,882 + flhE b1878 0.71 +0.4
1,960,882 + flhE b1878 0.71 -0.0
1,960,988 - -0.2
1,961,060 - -0.5
1,961,060 - +0.3
1,961,071 - +0.4
1,961,144 + +0.4
1,961,162 - +0.3
1,961,264 + flhA b1879 0.13 +2.4
1,961,329 - flhA b1879 0.16 -0.1
1,961,415 - flhA b1879 0.20 +0.5
1,961,422 + flhA b1879 0.20 +0.3
1,961,456 + flhA b1879 0.22 +0.1
1,961,513 + flhA b1879 0.25 +1.2
1,961,576 - flhA b1879 0.28 +0.0
1,961,576 - flhA b1879 0.28 +0.4
1,961,623 + flhA b1879 0.30 +0.7
1,961,644 - flhA b1879 0.31 -0.2
1,961,967 - flhA b1879 0.47 +0.1
1,962,000 + flhA b1879 0.48 +0.6
1,962,040 - flhA b1879 0.50 -0.2
1,962,040 - flhA b1879 0.50 +0.8
1,962,040 - flhA b1879 0.50 -0.2
1,962,154 + flhA b1879 0.56 -0.2
1,962,257 + flhA b1879 0.61 -0.0
1,962,265 - flhA b1879 0.61 -0.3
1,962,265 - flhA b1879 0.61 +1.2
1,962,513 + flhA b1879 0.73 +0.7
1,962,513 + flhA b1879 0.73 +0.4
1,962,958 - -0.4
1,963,140 - -0.7
1,963,140 - +0.6
1,963,206 + flhB b1880 0.12 +0.4
1,963,214 - flhB b1880 0.13 +0.0
1,963,214 - flhB b1880 0.13 +0.5
1,963,245 + flhB b1880 0.15 -0.7
1,963,349 - flhB b1880 0.25 +0.5
1,963,501 - flhB b1880 0.38 +0.5
1,963,599 + flhB b1880 0.46 +0.4
1,963,636 - flhB b1880 0.50 -0.8
1,963,647 - flhB b1880 0.50 +0.3
1,963,647 - flhB b1880 0.50 +0.2
1,963,705 - flhB b1880 0.56 +0.1
1,963,946 + flhB b1880 0.77 -0.7
1,964,064 - flhB b1880 0.87 +0.2

Or see this region's nucleotide sequence