Strain Fitness in Escherichia coli BW25113 around b1765

Experiment: Ying_all64 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntselD and ydjA are separated by 116 nucleotidesydjA and sppA are separated by 160 nucleotides b1764: selD - selenophosphate synthetase (NCBI), at 1,844,989 to 1,846,032 selD b1765: ydjA - predicted oxidoreductase (NCBI), at 1,846,149 to 1,846,700 ydjA b1766: sppA - protease IV (signal peptide peptidase) (NCBI), at 1,846,861 to 1,848,717 sppA Position (kb) 1846 1847Strain fitness (log2 ratio) -1 0 1at 1845.230 kb on - strand, within selDat 1845.242 kb on - strand, within selDat 1845.254 kb on - strand, within selDat 1845.300 kb on - strand, within selDat 1845.827 kb on - strand, within selDat 1845.827 kb on - strand, within selDat 1845.858 kb on - strand, within selDat 1845.881 kb on - strand, within selDat 1845.883 kb on - strand, within selDat 1846.260 kb on + strand, within ydjAat 1846.671 kb on - strandat 1846.671 kb on - strandat 1846.826 kb on - strandat 1846.828 kb on + strandat 1846.843 kb on - strandat 1846.849 kb on - strandat 1846.849 kb on - strandat 1846.860 kb on + strandat 1846.860 kb on + strandat 1846.910 kb on - strandat 1847.043 kb on - strandat 1847.186 kb on - strand, within sppAat 1847.251 kb on + strand, within sppAat 1847.251 kb on + strand, within sppAat 1847.481 kb on + strand, within sppAat 1847.481 kb on + strandat 1847.547 kb on - strand, within sppAat 1847.614 kb on - strand, within sppAat 1847.630 kb on - strand, within sppAat 1847.630 kb on - strand, within sppA

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2
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1,845,230 - selD b1764 0.23 -0.1
1,845,242 - selD b1764 0.24 +0.2
1,845,254 - selD b1764 0.25 +0.0
1,845,300 - selD b1764 0.30 -0.2
1,845,827 - selD b1764 0.80 -1.5
1,845,827 - selD b1764 0.80 +0.6
1,845,858 - selD b1764 0.83 +0.5
1,845,881 - selD b1764 0.85 +0.2
1,845,883 - selD b1764 0.86 -0.4
1,846,260 + ydjA b1765 0.20 +0.7
1,846,671 - -0.2
1,846,671 - -0.3
1,846,826 - +0.6
1,846,828 + +1.0
1,846,843 - -0.4
1,846,849 - +1.2
1,846,849 - -0.0
1,846,860 + -0.1
1,846,860 + -0.0
1,846,910 - -0.3
1,847,043 - +0.1
1,847,186 - sppA b1766 0.18 +0.9
1,847,251 + sppA b1766 0.21 +0.2
1,847,251 + sppA b1766 0.21 -0.3
1,847,481 + sppA b1766 0.33 +0.5
1,847,481 + -0.3
1,847,547 - sppA b1766 0.37 +0.5
1,847,614 - sppA b1766 0.41 +0.1
1,847,630 - sppA b1766 0.41 -0.5
1,847,630 - sppA b1766 0.41 +0.8

Or see this region's nucleotide sequence