Strain Fitness in Escherichia coli BW25113 around b1080

Experiment: Ying_all64 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgG and flgH are separated by 52 nucleotidesflgH and flgI are separated by 11 nucleotidesflgI and flgJ overlap by 1 nucleotides b1078: flgG - flagellar component of cell-distal portion of basal-body rod (NCBI), at 1,133,952 to 1,134,734 flgG b1079: flgH - flagellar L-ring protein precursor H (NCBI), at 1,134,787 to 1,135,485 flgH b1080: flgI - homolog of Salmonella P-ring of flagella basal body (VIMSS), at 1,135,497 to 1,136,594 flgI b1081: flgJ - flagellar biosynthesis protein FlgJ (NCBI), at 1,136,594 to 1,137,535 flgJ Position (kb) 1135 1136 1137Strain fitness (log2 ratio) -1 0 1at 1134.598 kb on - strand, within flgGat 1134.598 kb on - strand, within flgGat 1134.700 kb on - strandat 1134.752 kb on + strandat 1134.854 kb on - strandat 1134.857 kb on + strand, within flgHat 1134.893 kb on + strand, within flgHat 1134.893 kb on + strand, within flgHat 1134.901 kb on - strand, within flgHat 1135.012 kb on - strand, within flgHat 1135.101 kb on + strand, within flgHat 1135.257 kb on - strand, within flgHat 1135.634 kb on - strand, within flgIat 1135.838 kb on + strand, within flgIat 1135.849 kb on + strand, within flgIat 1135.867 kb on - strand, within flgIat 1135.913 kb on - strand, within flgIat 1136.103 kb on + strand, within flgIat 1136.129 kb on - strand, within flgIat 1136.129 kb on - strand, within flgIat 1136.165 kb on - strand, within flgIat 1136.168 kb on + strand, within flgIat 1136.312 kb on - strand, within flgIat 1136.312 kb on - strand, within flgIat 1136.319 kb on + strand, within flgIat 1136.335 kb on - strand, within flgIat 1136.399 kb on + strand, within flgIat 1136.413 kb on - strand, within flgIat 1136.451 kb on + strand, within flgIat 1136.507 kb on + strandat 1136.507 kb on + strandat 1136.620 kb on - strandat 1136.622 kb on - strandat 1136.622 kb on - strandat 1136.776 kb on + strand, within flgJat 1136.776 kb on + strand, within flgJat 1136.810 kb on + strand, within flgJat 1136.810 kb on + strand, within flgJat 1136.821 kb on + strand, within flgJat 1136.854 kb on - strand, within flgJat 1136.867 kb on - strand, within flgJat 1136.957 kb on - strand, within flgJat 1137.015 kb on - strand, within flgJat 1137.015 kb on - strand, within flgJat 1137.122 kb on - strand, within flgJat 1137.373 kb on - strand, within flgJat 1137.452 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2
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1,134,598 - flgG b1078 0.83 +0.8
1,134,598 - flgG b1078 0.83 -0.8
1,134,700 - -0.2
1,134,752 + -0.4
1,134,854 - +0.8
1,134,857 + flgH b1079 0.10 +0.1
1,134,893 + flgH b1079 0.15 -0.4
1,134,893 + flgH b1079 0.15 +0.8
1,134,901 - flgH b1079 0.16 +0.3
1,135,012 - flgH b1079 0.32 +0.9
1,135,101 + flgH b1079 0.45 +0.2
1,135,257 - flgH b1079 0.67 +0.3
1,135,634 - flgI b1080 0.12 +0.4
1,135,838 + flgI b1080 0.31 +0.2
1,135,849 + flgI b1080 0.32 +0.6
1,135,867 - flgI b1080 0.34 +0.7
1,135,913 - flgI b1080 0.38 +0.8
1,136,103 + flgI b1080 0.55 +0.2
1,136,129 - flgI b1080 0.58 +0.8
1,136,129 - flgI b1080 0.58 -0.0
1,136,165 - flgI b1080 0.61 -0.1
1,136,168 + flgI b1080 0.61 -0.0
1,136,312 - flgI b1080 0.74 -0.6
1,136,312 - flgI b1080 0.74 +0.4
1,136,319 + flgI b1080 0.75 +0.1
1,136,335 - flgI b1080 0.76 +0.2
1,136,399 + flgI b1080 0.82 +0.2
1,136,413 - flgI b1080 0.83 +0.3
1,136,451 + flgI b1080 0.87 +0.1
1,136,507 + +0.3
1,136,507 + +0.1
1,136,620 - -0.4
1,136,622 - -0.1
1,136,622 - +0.4
1,136,776 + flgJ b1081 0.19 +0.2
1,136,776 + flgJ b1081 0.19 -0.5
1,136,810 + flgJ b1081 0.23 -0.3
1,136,810 + flgJ b1081 0.23 -0.5
1,136,821 + flgJ b1081 0.24 +0.0
1,136,854 - flgJ b1081 0.28 -0.2
1,136,867 - flgJ b1081 0.29 -0.6
1,136,957 - flgJ b1081 0.39 -0.3
1,137,015 - flgJ b1081 0.45 -1.0
1,137,015 - flgJ b1081 0.45 -0.3
1,137,122 - flgJ b1081 0.56 -0.7
1,137,373 - flgJ b1081 0.83 -0.1
1,137,452 + +0.8

Or see this region's nucleotide sequence