Strain Fitness in Escherichia coli BW25113 around b0627

Experiment: Ying_all64 rep B; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybeM and tatE are separated by 128 nucleotidestatE and lipA are separated by 100 nucleotides b4581: ybeM - no description, at 657,254 to 658,041 ybeM b0627: tatE - twin arginine translocase protein E (NCBI), at 658,170 to 658,373 tatE b0628: lipA - lipoyl synthase (NCBI), at 658,474 to 659,439 lipA Position (kb) 658 659Strain fitness (log2 ratio) -2 -1 0 1 2at 657.208 kb on + strandat 657.425 kb on - strand, within ybeMat 657.782 kb on + strand, within ybeMat 657.836 kb on + strand, within ybeMat 657.836 kb on + strand, within ybeMat 657.836 kb on - strand, within ybeMat 657.898 kb on - strand, within ybeMat 657.917 kb on + strand, within ybeMat 657.945 kb on + strand, within ybeMat 657.963 kb on + strandat 657.963 kb on + strandat 658.011 kb on - strandat 658.163 kb on - strandat 658.218 kb on + strand, within tatEat 658.234 kb on - strand, within tatEat 658.234 kb on - strand, within tatEat 658.262 kb on + strand, within tatEat 658.262 kb on + strand, within tatEat 658.385 kb on + strandat 658.460 kb on - strandat 658.460 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2
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657,208 + -0.7
657,425 - ybeM b4581 0.22 -0.8
657,782 + ybeM b4581 0.67 +0.4
657,836 + ybeM b4581 0.74 -0.3
657,836 + ybeM b4581 0.74 -0.4
657,836 - ybeM b4581 0.74 +0.0
657,898 - ybeM b4581 0.82 +0.1
657,917 + ybeM b4581 0.84 -0.4
657,945 + ybeM b4581 0.88 +0.3
657,963 + +0.0
657,963 + +0.1
658,011 - +0.9
658,163 - +0.8
658,218 + tatE b0627 0.24 +0.5
658,234 - tatE b0627 0.31 +0.6
658,234 - tatE b0627 0.31 +1.8
658,262 + tatE b0627 0.45 -2.0
658,262 + tatE b0627 0.45 +0.3
658,385 + -0.2
658,460 - -2.0
658,460 - +1.1

Or see this region's nucleotide sequence