Experiment: Ying_all64 rep B; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgF and ykgG overlap by 8 nucleotides ykgG and ykgH are separated by 242 nucleotides ykgH and betA are separated by 212 nucleotides
b0307: ykgF - predicted amino acid dehydrogenase with NAD(P)-binding domain and ferridoxin-like domain (NCBI), at 321,562 to 322,989
ykgF
b0308: ykgG - putative transporter (VIMSS), at 322,982 to 323,677
ykgG
b0310: ykgH - predicted inner membrane protein (NCBI), at 323,920 to 324,588
ykgH
b0311: betA - choline dehydrogenase (NCBI), at 324,801 to 326,471
betA
Position (kb) 323
324
325 Strain fitness (log2 ratio) -2
-1
0
1
2 at 323.074 kb on + strand, within ykgG at 323.074 kb on + strand, within ykgG at 323.094 kb on + strand, within ykgG at 323.105 kb on + strand, within ykgG at 323.168 kb on + strand, within ykgG at 323.237 kb on + strand, within ykgG at 323.307 kb on - strand, within ykgG at 323.346 kb on - strand, within ykgG at 323.346 kb on - strand, within ykgG at 323.359 kb on + strand, within ykgG at 323.359 kb on + strand, within ykgG at 323.500 kb on + strand, within ykgG at 323.503 kb on + strand, within ykgG at 323.503 kb on - strand, within ykgG at 323.583 kb on - strand, within ykgG at 323.612 kb on - strand at 323.614 kb on + strand at 323.614 kb on + strand at 323.621 kb on - strand at 323.623 kb on + strand at 323.623 kb on + strand at 323.623 kb on + strand at 323.628 kb on + strand at 323.628 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.651 kb on + strand at 323.690 kb on + strand at 323.696 kb on + strand at 323.698 kb on - strand at 323.701 kb on + strand at 323.701 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.742 kb on + strand at 323.748 kb on + strand at 323.748 kb on + strand at 323.748 kb on + strand at 323.749 kb on + strand at 323.757 kb on + strand at 323.757 kb on + strand at 323.759 kb on + strand at 323.759 kb on + strand at 323.759 kb on + strand at 323.874 kb on + strand at 323.874 kb on + strand at 323.889 kb on + strand at 323.892 kb on - strand at 323.892 kb on - strand at 323.892 kb on - strand at 323.892 kb on - strand at 324.068 kb on - strand, within ykgH at 324.068 kb on - strand, within ykgH at 324.070 kb on - strand, within ykgH at 324.088 kb on - strand, within ykgH at 324.095 kb on - strand, within ykgH at 324.248 kb on - strand, within ykgH at 324.636 kb on - strand at 324.648 kb on - strand at 324.648 kb on - strand at 324.648 kb on - strand at 324.691 kb on - strand at 324.701 kb on - strand at 324.701 kb on - strand at 324.710 kb on - strand at 324.729 kb on - strand at 324.740 kb on - strand at 324.749 kb on - strand at 324.749 kb on - strand at 324.754 kb on - strand at 324.754 kb on - strand at 324.754 kb on - strand at 324.754 kb on - strand at 324.754 kb on - strand at 324.768 kb on - strand at 324.768 kb on - strand at 324.768 kb on - strand at 324.783 kb on - strand at 324.792 kb on - strand at 324.794 kb on - strand at 324.824 kb on - strand at 324.855 kb on - strand at 324.863 kb on - strand at 324.863 kb on - strand at 324.882 kb on - strand at 324.888 kb on - strand at 324.968 kb on - strand, within betA at 324.973 kb on - strand, within betA at 324.975 kb on - strand, within betA at 324.985 kb on - strand, within betA at 324.985 kb on - strand, within betA at 324.992 kb on - strand, within betA at 325.078 kb on + strand, within betA at 325.156 kb on - strand, within betA at 325.156 kb on - strand, within betA at 325.222 kb on + strand, within betA at 325.222 kb on + strand, within betA at 325.278 kb on - strand, within betA at 325.278 kb on - strand, within betA at 325.332 kb on - strand, within betA at 325.332 kb on - strand, within betA at 325.345 kb on - strand, within betA at 325.370 kb on - strand, within betA at 325.370 kb on - strand, within betA at 325.446 kb on - strand, within betA at 325.506 kb on + strand, within betA at 325.513 kb on - strand, within betA at 325.513 kb on - strand, within betA at 325.513 kb on - strand, within betA at 325.520 kb on + strand, within betA at 325.520 kb on + strand, within betA at 325.525 kb on + strand, within betA at 325.525 kb on - strand, within betA at 325.533 kb on - strand, within betA at 325.533 kb on - strand, within betA at 325.551 kb on - strand, within betA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep B; time point 2 remove 323,074 + ykgG b0308 0.13 -0.1 323,074 + ykgG b0308 0.13 -0.3 323,094 + ykgG b0308 0.16 +0.5 323,105 + ykgG b0308 0.18 -0.1 323,168 + ykgG b0308 0.27 -0.1 323,237 + ykgG b0308 0.37 +0.2 323,307 - ykgG b0308 0.47 +0.6 323,346 - ykgG b0308 0.52 +1.6 323,346 - ykgG b0308 0.52 +0.8 323,359 + ykgG b0308 0.54 -0.1 323,359 + ykgG b0308 0.54 +0.2 323,500 + ykgG b0308 0.74 -0.1 323,503 + ykgG b0308 0.75 +0.5 323,503 - ykgG b0308 0.75 +1.6 323,583 - ykgG b0308 0.86 -0.1 323,612 - -1.2 323,614 + +0.5 323,614 + -0.3 323,621 - -0.2 323,623 + +0.5 323,623 + +0.2 323,623 + +0.2 323,628 + -0.0 323,628 + -0.7 323,630 + +0.1 323,630 + -0.1 323,630 + -0.0 323,630 + -0.2 323,630 + -0.0 323,630 + -0.0 323,630 + -0.3 323,651 + -0.1 323,690 + +0.2 323,696 + -0.2 323,698 - +2.6 323,701 + +0.5 323,701 + +0.7 323,712 + -0.3 323,712 + -0.3 323,712 + -0.5 323,712 + +0.0 323,742 + +0.1 323,748 + -0.6 323,748 + -1.0 323,748 + -0.3 323,749 + -0.4 323,757 + +0.9 323,757 + -0.0 323,759 + +1.1 323,759 + -1.0 323,759 + +0.4 323,874 + +0.6 323,874 + +0.8 323,889 + +1.0 323,892 - +2.6 323,892 - +1.0 323,892 - +2.6 323,892 - +0.9 324,068 - ykgH b0310 0.22 +2.6 324,068 - ykgH b0310 0.22 -0.4 324,070 - ykgH b0310 0.22 +0.8 324,088 - ykgH b0310 0.25 +2.6 324,095 - ykgH b0310 0.26 +2.6 324,248 - ykgH b0310 0.49 +2.6 324,636 - -0.5 324,648 - -0.6 324,648 - +0.4 324,648 - +0.4 324,691 - +0.5 324,701 - -0.7 324,701 - +1.2 324,710 - -0.9 324,729 - +0.5 324,740 - -0.6 324,749 - -0.2 324,749 - -2.5 324,754 - +1.2 324,754 - -1.1 324,754 - -0.7 324,754 - -1.9 324,754 - +0.7 324,768 - -0.4 324,768 - +0.9 324,768 - +1.1 324,783 - -0.3 324,792 - -0.1 324,794 - -0.2 324,824 - +0.1 324,855 - +0.2 324,863 - +0.3 324,863 - -0.3 324,882 - +0.2 324,888 - -0.2 324,968 - betA b0311 0.10 +0.5 324,973 - betA b0311 0.10 -0.3 324,975 - betA b0311 0.10 +0.2 324,985 - betA b0311 0.11 +0.5 324,985 - betA b0311 0.11 +0.0 324,992 - betA b0311 0.11 +0.0 325,078 + betA b0311 0.17 +0.7 325,156 - betA b0311 0.21 -0.4 325,156 - betA b0311 0.21 +0.5 325,222 + betA b0311 0.25 +0.1 325,222 + betA b0311 0.25 +0.2 325,278 - betA b0311 0.29 -0.1 325,278 - betA b0311 0.29 -0.3 325,332 - betA b0311 0.32 -0.1 325,332 - betA b0311 0.32 +0.5 325,345 - betA b0311 0.33 +0.1 325,370 - betA b0311 0.34 +0.6 325,370 - betA b0311 0.34 +0.2 325,446 - betA b0311 0.39 -0.1 325,506 + betA b0311 0.42 -0.6 325,513 - betA b0311 0.43 +0.0 325,513 - betA b0311 0.43 -0.3 325,513 - betA b0311 0.43 +0.4 325,520 + betA b0311 0.43 +0.3 325,520 + betA b0311 0.43 -0.2 325,525 + betA b0311 0.43 +0.4 325,525 - betA b0311 0.43 +0.1 325,533 - betA b0311 0.44 +1.1 325,533 - betA b0311 0.44 -0.3 325,551 - betA b0311 0.45 -0.7
Or see this region's nucleotide sequence