Experiment: Ying_all64 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and fliQ are separated by 9 nucleotides fliQ and fliR are separated by 7 nucleotides fliR and rcsA are separated by 289 nucleotides rcsA and dsrB are separated by 43 nucleotides
b1948: fliP - flagellar biosynthesis protein P (NCBI), at 2,019,893 to 2,020,630
fliP
b1949: fliQ - flagellar biosynthesis protein Q (NCBI), at 2,020,640 to 2,020,909
fliQ
b1950: fliR - flagellar biosynthesis protein R (NCBI), at 2,020,917 to 2,021,702
fliR
b1951: rcsA - DNA-binding transcriptional activator, co-regulator with RcsB (NCBI), at 2,021,992 to 2,022,615
rcsA
b1952: dsrB - hypothetical protein (NCBI), at 2,022,659 to 2,022,847
dsrB
Position (kb)
2020
2021
2022 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2019.995 kb on + strand, within fliP at 2020.000 kb on + strand, within fliP at 2020.231 kb on - strand, within fliP at 2020.359 kb on - strand, within fliP at 2020.359 kb on - strand, within fliP at 2020.375 kb on + strand, within fliP at 2020.382 kb on - strand, within fliP at 2020.668 kb on + strand, within fliQ at 2020.719 kb on - strand, within fliQ at 2020.840 kb on + strand, within fliQ at 2020.894 kb on + strand at 2020.899 kb on - strand at 2020.950 kb on - strand at 2021.298 kb on + strand, within fliR at 2021.377 kb on + strand, within fliR at 2021.377 kb on + strand, within fliR at 2021.413 kb on - strand, within fliR at 2021.498 kb on + strand, within fliR at 2021.501 kb on + strand, within fliR at 2021.501 kb on + strand, within fliR at 2021.522 kb on - strand, within fliR at 2021.572 kb on + strand, within fliR at 2021.594 kb on + strand, within fliR at 2021.594 kb on + strand, within fliR at 2021.595 kb on + strand, within fliR at 2021.595 kb on + strand, within fliR at 2021.812 kb on - strand at 2021.982 kb on + strand at 2021.982 kb on + strand at 2022.049 kb on + strand at 2022.191 kb on + strand, within rcsA at 2022.382 kb on - strand, within rcsA at 2022.382 kb on - strand, within rcsA at 2022.413 kb on - strand, within rcsA at 2022.413 kb on - strand, within rcsA at 2022.424 kb on - strand, within rcsA at 2022.424 kb on - strand, within rcsA at 2022.427 kb on - strand, within rcsA at 2022.435 kb on + strand, within rcsA at 2022.453 kb on - strand, within rcsA at 2022.456 kb on - strand, within rcsA at 2022.610 kb on + strand at 2022.610 kb on + strand at 2022.627 kb on - strand at 2022.637 kb on - strand at 2022.650 kb on - strand at 2022.650 kb on - strand at 2022.674 kb on - strand at 2022.674 kb on - strand at 2022.694 kb on + strand, within dsrB at 2022.694 kb on + strand, within dsrB
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2 remove 2,019,995 + fliP b1948 0.14 +0.2 2,020,000 + fliP b1948 0.14 +0.2 2,020,231 - fliP b1948 0.46 +0.1 2,020,359 - fliP b1948 0.63 -0.1 2,020,359 - fliP b1948 0.63 -0.3 2,020,375 + fliP b1948 0.65 +0.3 2,020,382 - fliP b1948 0.66 +0.3 2,020,668 + fliQ b1949 0.10 +0.4 2,020,719 - fliQ b1949 0.29 +0.3 2,020,840 + fliQ b1949 0.74 +0.1 2,020,894 + +0.4 2,020,899 - +0.1 2,020,950 - +0.1 2,021,298 + fliR b1950 0.48 +0.3 2,021,377 + fliR b1950 0.59 +0.7 2,021,377 + fliR b1950 0.59 +0.5 2,021,413 - fliR b1950 0.63 +0.3 2,021,498 + fliR b1950 0.74 +0.5 2,021,501 + fliR b1950 0.74 +0.7 2,021,501 + fliR b1950 0.74 +0.4 2,021,522 - fliR b1950 0.77 +2.1 2,021,572 + fliR b1950 0.83 +1.5 2,021,594 + fliR b1950 0.86 +1.1 2,021,594 + fliR b1950 0.86 +2.4 2,021,595 + fliR b1950 0.86 -1.0 2,021,595 + fliR b1950 0.86 +0.9 2,021,812 - +3.7 2,021,982 + +2.1 2,021,982 + +2.1 2,022,049 + +0.1 2,022,191 + rcsA b1951 0.32 +0.5 2,022,382 - rcsA b1951 0.62 +3.1 2,022,382 - rcsA b1951 0.62 +1.7 2,022,413 - rcsA b1951 0.67 -1.6 2,022,413 - rcsA b1951 0.67 +0.5 2,022,424 - rcsA b1951 0.69 +1.1 2,022,424 - rcsA b1951 0.69 +0.5 2,022,427 - rcsA b1951 0.70 -0.9 2,022,435 + rcsA b1951 0.71 +2.1 2,022,453 - rcsA b1951 0.74 -0.3 2,022,456 - rcsA b1951 0.74 -0.2 2,022,610 + +1.1 2,022,610 + +0.8 2,022,627 - -0.0 2,022,637 - -0.1 2,022,650 - +0.0 2,022,650 - -0.6 2,022,674 - -0.6 2,022,674 - +0.6 2,022,694 + dsrB b1952 0.19 +0.7 2,022,694 + dsrB b1952 0.19 +1.0
Or see this region's nucleotide sequence