Strain Fitness in Escherichia coli BW25113 around b1273

Experiment: Ying_all64 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsohB and yciN are separated by 35 nucleotidesyciN and topA are separated by 379 nucleotides b1272: sohB - predicted inner membrane peptidase (NCBI), at 1,327,356 to 1,328,405 sohB b1273: yciN - hypothetical protein (NCBI), at 1,328,441 to 1,328,692 yciN b1274: topA - DNA topoisomerase I (NCBI), at 1,329,072 to 1,331,669 topA Position (kb) 1328 1329Strain fitness (log2 ratio) -1 0 1 2at 1327.500 kb on + strand, within sohBat 1327.505 kb on + strand, within sohBat 1327.505 kb on + strand, within sohBat 1327.511 kb on - strand, within sohBat 1327.511 kb on - strand, within sohBat 1327.565 kb on + strand, within sohBat 1327.565 kb on + strand, within sohBat 1327.752 kb on - strand, within sohBat 1327.752 kb on - strand, within sohBat 1327.797 kb on + strand, within sohBat 1327.797 kb on + strand, within sohBat 1327.902 kb on - strand, within sohBat 1328.003 kb on + strand, within sohBat 1328.032 kb on - strand, within sohBat 1328.039 kb on + strand, within sohBat 1328.039 kb on + strand, within sohBat 1328.144 kb on + strand, within sohBat 1328.162 kb on + strand, within sohBat 1328.202 kb on - strand, within sohBat 1328.211 kb on + strand, within sohBat 1328.211 kb on + strand, within sohBat 1328.364 kb on + strandat 1328.556 kb on + strand, within yciNat 1328.569 kb on + strand, within yciNat 1328.713 kb on + strandat 1328.713 kb on + strandat 1328.829 kb on + strandat 1328.918 kb on - strandat 1328.962 kb on - strandat 1328.965 kb on + strandat 1328.965 kb on + strandat 1329.065 kb on + strandat 1329.065 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2
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1,327,500 + sohB b1272 0.14 +0.0
1,327,505 + sohB b1272 0.14 +0.1
1,327,505 + sohB b1272 0.14 +0.0
1,327,511 - sohB b1272 0.15 +1.0
1,327,511 - sohB b1272 0.15 +0.8
1,327,565 + sohB b1272 0.20 +0.2
1,327,565 + sohB b1272 0.20 +1.4
1,327,752 - sohB b1272 0.38 -0.6
1,327,752 - sohB b1272 0.38 -1.4
1,327,797 + sohB b1272 0.42 -0.4
1,327,797 + sohB b1272 0.42 -0.3
1,327,902 - sohB b1272 0.52 -0.4
1,328,003 + sohB b1272 0.62 -0.2
1,328,032 - sohB b1272 0.64 +0.1
1,328,039 + sohB b1272 0.65 -0.2
1,328,039 + sohB b1272 0.65 +0.5
1,328,144 + sohB b1272 0.75 -0.0
1,328,162 + sohB b1272 0.77 +0.2
1,328,202 - sohB b1272 0.81 +0.1
1,328,211 + sohB b1272 0.81 -0.0
1,328,211 + sohB b1272 0.81 -0.0
1,328,364 + -0.1
1,328,556 + yciN b1273 0.46 +0.3
1,328,569 + yciN b1273 0.51 +2.1
1,328,713 + -0.3
1,328,713 + -0.7
1,328,829 + -1.5
1,328,918 - +0.4
1,328,962 - -0.2
1,328,965 + -0.9
1,328,965 + -1.1
1,329,065 + -0.2
1,329,065 + +0.3

Or see this region's nucleotide sequence