Experiment: Ying_all64 rep A; time point 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prpE and codB are separated by 329 nucleotides codB and codA overlap by 11 nucleotides
b0335: prpE - predicted propionyl-CoA synthetase with ATPase domain (NCBI), at 351,930 to 353,816
prpE
b0336: codB - cytosine transporter (NCBI), at 354,146 to 355,405
codB
b0337: codA - cytosine deaminase (NCBI), at 355,395 to 356,678
codA
Position (kb) 354
355
356 Strain fitness (log2 ratio) -1
0
1 at 353.151 kb on + strand, within prpE at 353.151 kb on + strand, within prpE at 353.197 kb on + strand, within prpE at 353.241 kb on - strand, within prpE at 353.346 kb on + strand, within prpE at 353.346 kb on + strand, within prpE at 353.363 kb on - strand, within prpE at 353.440 kb on + strand, within prpE at 353.465 kb on + strand, within prpE at 353.589 kb on + strand, within prpE at 353.589 kb on + strand, within prpE at 353.746 kb on + strand at 353.957 kb on + strand at 353.966 kb on - strand at 354.098 kb on - strand at 354.174 kb on + strand at 354.254 kb on + strand at 354.311 kb on + strand, within codB at 354.384 kb on + strand, within codB at 354.386 kb on - strand, within codB at 354.423 kb on - strand, within codB at 354.431 kb on - strand, within codB at 354.480 kb on + strand, within codB at 354.773 kb on - strand, within codB at 354.773 kb on - strand, within codB at 354.885 kb on + strand, within codB at 354.893 kb on - strand, within codB at 354.935 kb on - strand, within codB at 355.005 kb on - strand, within codB at 355.082 kb on - strand, within codB at 355.086 kb on + strand, within codB at 355.153 kb on - strand, within codB at 355.220 kb on - strand, within codB at 355.262 kb on - strand, within codB at 355.265 kb on + strand, within codB at 355.307 kb on + strand at 355.315 kb on - strand at 355.337 kb on - strand at 355.462 kb on - strand at 355.463 kb on - strand at 355.463 kb on - strand at 355.592 kb on - strand, within codA at 355.764 kb on - strand, within codA at 355.764 kb on - strand, within codA at 355.790 kb on - strand, within codA at 355.825 kb on - strand, within codA at 355.996 kb on - strand, within codA at 355.996 kb on - strand, within codA at 356.088 kb on + strand, within codA at 356.150 kb on + strand, within codA at 356.153 kb on + strand, within codA at 356.178 kb on - strand, within codA at 356.295 kb on - strand, within codA at 356.295 kb on - strand, within codA at 356.306 kb on - strand, within codA at 356.342 kb on - strand, within codA at 356.390 kb on + strand, within codA at 356.390 kb on + strand, within codA
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2 remove 353,151 + prpE b0335 0.65 -1.6 353,151 + prpE b0335 0.65 +0.5 353,197 + prpE b0335 0.67 +0.0 353,241 - prpE b0335 0.69 -0.0 353,346 + prpE b0335 0.75 +0.0 353,346 + prpE b0335 0.75 +0.1 353,363 - prpE b0335 0.76 +0.5 353,440 + prpE b0335 0.80 -0.2 353,465 + prpE b0335 0.81 -0.1 353,589 + prpE b0335 0.88 +0.2 353,589 + prpE b0335 0.88 +0.4 353,746 + -0.5 353,957 + +0.5 353,966 - -0.2 354,098 - +0.2 354,174 + +0.1 354,254 + +0.3 354,311 + codB b0336 0.13 +0.5 354,384 + codB b0336 0.19 +0.5 354,386 - codB b0336 0.19 +0.4 354,423 - codB b0336 0.22 +0.2 354,431 - codB b0336 0.23 +0.5 354,480 + codB b0336 0.27 -0.0 354,773 - codB b0336 0.50 +0.9 354,773 - codB b0336 0.50 +0.5 354,885 + codB b0336 0.59 +0.2 354,893 - codB b0336 0.59 +0.0 354,935 - codB b0336 0.63 +0.4 355,005 - codB b0336 0.68 -0.4 355,082 - codB b0336 0.74 +0.5 355,086 + codB b0336 0.75 -0.2 355,153 - codB b0336 0.80 +0.2 355,220 - codB b0336 0.85 +0.8 355,262 - codB b0336 0.89 +0.1 355,265 + codB b0336 0.89 +0.2 355,307 + -0.5 355,315 - +0.1 355,337 - +0.4 355,462 - -0.1 355,463 - +0.4 355,463 - -0.2 355,592 - codA b0337 0.15 -0.4 355,764 - codA b0337 0.29 +0.2 355,764 - codA b0337 0.29 +0.1 355,790 - codA b0337 0.31 -0.0 355,825 - codA b0337 0.33 -0.2 355,996 - codA b0337 0.47 +0.2 355,996 - codA b0337 0.47 +0.6 356,088 + codA b0337 0.54 -0.1 356,150 + codA b0337 0.59 -0.2 356,153 + codA b0337 0.59 +0.3 356,178 - codA b0337 0.61 -0.0 356,295 - codA b0337 0.70 +0.1 356,295 - codA b0337 0.70 -0.5 356,306 - codA b0337 0.71 +0.0 356,342 - codA b0337 0.74 +0.2 356,390 + codA b0337 0.77 +0.2 356,390 + codA b0337 0.77 +0.1
Or see this region's nucleotide sequence