Strain Fitness in Escherichia coli BW25113 around b0227

Experiment: Ying_all64 rep A; time point 2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyafK and yafQ are separated by 155 nucleotidesyafQ and dinJ are separated by 2 nucleotidesdinJ and yafL are separated by 209 nucleotidesyafL and yafM are separated by 175 nucleotidesyafM and lfhA are separated by 223 nucleotides b0224: yafK - hypothetical protein (NCBI), at 245,065 to 245,805 yafK b0225: yafQ - predicted toxin of the YafQ-DinJ toxin-antitoxin system (NCBI), at 245,961 to 246,239 yafQ b0226: dinJ - predicted antitoxin of YafQ-DinJ toxin-antitoxin system (NCBI), at 246,242 to 246,502 dinJ b0227: yafL - predicted lipoprotein and C40 family peptidase (NCBI), at 246,712 to 247,461 yafL b0228: yafM - hypothetical protein (NCBI), at 247,637 to 248,134 yafM b0229: lfhA - flagellar biosynthesis (VIMSS), at 248,358 to 250,070 lfhA Position (kb) 246 247 248Strain fitness (log2 ratio) -1 0 1 2 3at 245.735 kb on - strandat 245.743 kb on - strandat 245.743 kb on - strandat 245.837 kb on + strandat 245.928 kb on - strandat 245.928 kb on - strandat 245.964 kb on + strandat 245.964 kb on + strandat 245.971 kb on - strandat 246.048 kb on + strand, within yafQat 246.048 kb on + strand, within yafQat 246.048 kb on + strand, within yafQat 246.086 kb on - strand, within yafQat 246.086 kb on - strand, within yafQat 246.086 kb on - strand, within yafQat 246.086 kb on - strand, within yafQat 246.097 kb on - strand, within yafQat 246.294 kb on - strand, within dinJat 246.295 kb on - strand, within dinJat 246.304 kb on - strand, within dinJat 246.330 kb on - strand, within dinJat 246.338 kb on - strand, within dinJat 246.338 kb on - strand, within dinJat 246.411 kb on + strand, within dinJat 246.411 kb on + strand, within dinJat 246.742 kb on - strandat 246.821 kb on - strand, within yafLat 246.854 kb on + strand, within yafLat 246.859 kb on + strand, within yafLat 247.129 kb on - strand, within yafLat 247.129 kb on - strand, within yafLat 247.227 kb on - strand, within yafLat 247.227 kb on - strand, within yafLat 247.288 kb on + strand, within yafLat 247.312 kb on - strand, within yafLat 247.409 kb on - strandat 247.493 kb on + strandat 247.502 kb on - strandat 247.729 kb on + strand, within yafMat 247.786 kb on - strand, within yafMat 247.786 kb on - strand, within yafMat 247.797 kb on - strand, within yafMat 247.824 kb on - strand, within yafMat 247.831 kb on - strand, within yafMat 248.035 kb on + strand, within yafMat 248.067 kb on + strand, within yafMat 248.185 kb on - strandat 248.185 kb on - strandat 248.336 kb on + strandat 248.337 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_all64 rep A; time point 2
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245,735 - +0.4
245,743 - -0.2
245,743 - +0.7
245,837 + +0.2
245,928 - -0.9
245,928 - -0.4
245,964 + +0.4
245,964 + -0.1
245,971 - +0.1
246,048 + yafQ b0225 0.31 +0.4
246,048 + yafQ b0225 0.31 -0.2
246,048 + yafQ b0225 0.31 +0.4
246,086 - yafQ b0225 0.45 -0.4
246,086 - yafQ b0225 0.45 +0.5
246,086 - yafQ b0225 0.45 +0.8
246,086 - yafQ b0225 0.45 +2.9
246,097 - yafQ b0225 0.49 +0.0
246,294 - dinJ b0226 0.20 -0.2
246,295 - dinJ b0226 0.20 -0.5
246,304 - dinJ b0226 0.24 -0.8
246,330 - dinJ b0226 0.34 -0.7
246,338 - dinJ b0226 0.37 +0.0
246,338 - dinJ b0226 0.37 +0.3
246,411 + dinJ b0226 0.65 -1.2
246,411 + dinJ b0226 0.65 -0.2
246,742 - +0.2
246,821 - yafL b0227 0.15 +0.0
246,854 + yafL b0227 0.19 +0.1
246,859 + yafL b0227 0.20 -0.2
247,129 - yafL b0227 0.56 +0.2
247,129 - yafL b0227 0.56 +1.6
247,227 - yafL b0227 0.69 +0.1
247,227 - yafL b0227 0.69 +0.2
247,288 + yafL b0227 0.77 +0.0
247,312 - yafL b0227 0.80 +0.4
247,409 - -0.2
247,493 + -0.5
247,502 - -0.1
247,729 + yafM b0228 0.18 +0.0
247,786 - yafM b0228 0.30 -0.0
247,786 - yafM b0228 0.30 +0.2
247,797 - yafM b0228 0.32 +0.5
247,824 - yafM b0228 0.38 -0.3
247,831 - yafM b0228 0.39 +0.3
248,035 + yafM b0228 0.80 +0.1
248,067 + yafM b0228 0.86 +0.1
248,185 - -0.0
248,185 - +0.1
248,336 + -0.3
248,337 - +0.1

Or see this region's nucleotide sequence