Experiment: Ying_Others16 rep B; time point 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pyrF and yciH overlap by 1 nucleotides yciH and osmB are separated by 125 nucleotides osmB and yciT are separated by 268 nucleotides yciT and yciZ are separated by 89 nucleotides yciZ and gmr are separated by 147 nucleotides
b1281: pyrF - orotidine 5'-phosphate decarboxylase (NCBI), at 1,339,945 to 1,340,682
pyrF
b1282: yciH - orf, hypothetical protein (VIMSS), at 1,340,682 to 1,341,008
yciH
b1283: osmB - lipoprotein (NCBI), at 1,341,134 to 1,341,352
osmB
b1284: yciT - predicted DNA-binding transcriptional regulator (NCBI), at 1,341,621 to 1,342,370
yciT
b4596: yciZ - hypothetical protein (RefSeq), at 1,342,460 to 1,342,633
yciZ
b1285: gmr - modulator of Rnase II stability (NCBI), at 1,342,781 to 1,344,766
gmr
Position (kb)
1341
1342
1343 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1340.691 kb on + strand at 1340.708 kb on - strand at 1340.708 kb on - strand at 1340.748 kb on + strand, within yciH at 1340.781 kb on - strand, within yciH at 1340.902 kb on + strand, within yciH at 1340.937 kb on + strand, within yciH at 1340.940 kb on + strand, within yciH at 1340.986 kb on + strand at 1341.128 kb on - strand at 1341.179 kb on - strand, within osmB at 1341.179 kb on - strand, within osmB at 1341.187 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.203 kb on - strand, within osmB at 1341.211 kb on + strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.223 kb on - strand, within osmB at 1341.229 kb on - strand, within osmB at 1341.274 kb on + strand, within osmB at 1341.274 kb on + strand, within osmB at 1341.282 kb on - strand, within osmB at 1341.288 kb on + strand, within osmB at 1341.293 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.369 kb on - strand at 1341.369 kb on - strand at 1341.400 kb on - strand at 1341.421 kb on - strand at 1341.459 kb on - strand at 1341.465 kb on - strand at 1341.473 kb on - strand at 1341.473 kb on - strand at 1341.483 kb on - strand at 1341.494 kb on - strand at 1341.495 kb on - strand at 1341.495 kb on - strand at 1341.543 kb on - strand at 1341.585 kb on - strand at 1341.585 kb on - strand at 1341.609 kb on - strand at 1341.632 kb on - strand at 1341.645 kb on - strand at 1341.654 kb on + strand at 1341.654 kb on + strand at 1341.656 kb on + strand at 1341.719 kb on + strand, within yciT at 1341.719 kb on + strand, within yciT at 1341.725 kb on - strand, within yciT at 1341.768 kb on + strand, within yciT at 1341.823 kb on - strand, within yciT at 1342.104 kb on + strand, within yciT at 1342.104 kb on + strand, within yciT at 1342.216 kb on - strand, within yciT at 1342.306 kb on - strand at 1342.313 kb on + strand at 1342.313 kb on + strand at 1342.329 kb on + strand at 1342.478 kb on + strand, within yciZ at 1342.478 kb on + strand, within yciZ at 1342.708 kb on - strand at 1342.809 kb on - strand at 1343.191 kb on - strand, within gmr at 1343.224 kb on + strand, within gmr at 1343.224 kb on + strand, within gmr at 1343.235 kb on + strand, within gmr
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_Others16 rep B; time point 1 remove 1,340,691 + +0.5 1,340,708 - +1.0 1,340,708 - +0.6 1,340,748 + yciH b1282 0.20 +0.2 1,340,781 - yciH b1282 0.30 +1.3 1,340,902 + yciH b1282 0.67 +0.2 1,340,937 + yciH b1282 0.78 -0.1 1,340,940 + yciH b1282 0.79 +0.2 1,340,986 + +1.1 1,341,128 - -0.3 1,341,179 - osmB b1283 0.21 -0.2 1,341,179 - osmB b1283 0.21 +0.3 1,341,187 - osmB b1283 0.24 -0.2 1,341,191 - osmB b1283 0.26 +0.3 1,341,191 - osmB b1283 0.26 -2.6 1,341,191 - osmB b1283 0.26 -1.5 1,341,203 - osmB b1283 0.32 -0.2 1,341,211 + osmB b1283 0.35 +1.4 1,341,214 - osmB b1283 0.37 +0.0 1,341,214 - osmB b1283 0.37 -2.0 1,341,214 - osmB b1283 0.37 +0.5 1,341,214 - osmB b1283 0.37 -2.3 1,341,214 - osmB b1283 0.37 -2.1 1,341,214 - osmB b1283 0.37 +0.0 1,341,219 - osmB b1283 0.39 -2.4 1,341,219 - osmB b1283 0.39 -0.8 1,341,219 - osmB b1283 0.39 +1.7 1,341,219 - osmB b1283 0.39 -2.3 1,341,219 - osmB b1283 0.39 +0.2 1,341,223 - osmB b1283 0.41 +0.2 1,341,229 - osmB b1283 0.43 -0.2 1,341,274 + osmB b1283 0.64 +0.4 1,341,274 + osmB b1283 0.64 +1.4 1,341,282 - osmB b1283 0.68 +0.4 1,341,288 + osmB b1283 0.70 +2.4 1,341,293 - osmB b1283 0.73 -0.5 1,341,296 - osmB b1283 0.74 +1.0 1,341,296 - osmB b1283 0.74 +0.7 1,341,296 - osmB b1283 0.74 +1.4 1,341,296 - osmB b1283 0.74 +1.5 1,341,369 - +2.5 1,341,369 - +1.5 1,341,400 - -3.7 1,341,421 - +0.0 1,341,459 - +1.0 1,341,465 - -0.3 1,341,473 - +0.8 1,341,473 - -1.0 1,341,483 - -0.1 1,341,494 - +0.7 1,341,495 - -0.1 1,341,495 - -0.3 1,341,543 - -1.7 1,341,585 - +0.8 1,341,585 - +1.2 1,341,609 - +1.0 1,341,632 - +0.6 1,341,645 - +0.3 1,341,654 + +1.1 1,341,654 + -0.8 1,341,656 + +0.9 1,341,719 + yciT b1284 0.13 +1.0 1,341,719 + yciT b1284 0.13 +1.7 1,341,725 - yciT b1284 0.14 +0.5 1,341,768 + yciT b1284 0.20 +1.1 1,341,823 - yciT b1284 0.27 +0.8 1,342,104 + yciT b1284 0.64 +0.6 1,342,104 + yciT b1284 0.64 +0.1 1,342,216 - yciT b1284 0.79 +1.0 1,342,306 - +0.5 1,342,313 + +0.1 1,342,313 + +1.4 1,342,329 + +0.6 1,342,478 + yciZ b4596 0.10 +0.6 1,342,478 + yciZ b4596 0.10 -0.1 1,342,708 - -0.1 1,342,809 - -0.4 1,343,191 - gmr b1285 0.21 +0.2 1,343,224 + gmr b1285 0.22 -0.5 1,343,224 + gmr b1285 0.22 -2.0 1,343,235 + gmr b1285 0.23 -0.0
Or see this region's nucleotide sequence